Term
| Reverse transcriptase is an enzyme normally used by RNA viruses to make DNA from their RNA; researchers use the enzyme to make cDNA from the RNA produced by cells |
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Term
| DNA fingerprints are based on the occurrence of variable-sized fragments of DNA (tandem repeats) in each individual |
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Term
| Because DNA works by the same general principles in all organisms, it is possible to isolate a gene from one species, and artificially place it into another |
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Which of the following is required for PCR?
All of these
Short "primers" from each end of the target DNA
DNA region to be amplified
Nucleotides (A,C,G, and T)
DNA polymerase |
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Term
| A cDNA library contains only the portion of the DNA that is transcribed into RNA |
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Definition
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Term
| A laboratory method used to generate a large number of copies of a particular segment of DNA is called: |
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Definition
| Polymerase Chain Reaction (PCR) |
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Term
| A plasmid is a small, circular, extra-chromosomal piece of DNA that occurs in many bacteria |
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Definition
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Which of the following is a transgenic experiment that has been successfully carried out?
Salmon containing an antifreeze gene.
Pigs that contain human antithrombi III.
Goats that produce milk containing spider silk.
Rabbits containing the human interleukin-2 gene
All of these. |
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Definition
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Term
| A "cloning vector" is the name given to researchers who take DNA from one species and place it into another |
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Definition
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Term
| DNA fingerprinting is done by generating fragments of DNA using restriction enzymes; the resulting fragments may show Restriction Fragment Length Polymorphisms (RFLP's) |
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Definition
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Term
| Automated DNA sequencing may be used to determine the nucleotide sequence of a portion of isolated DNA |
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Term
| The transfer of genes from one species to another can only be done in a laboratory. There are no known cases of genes moving from one species to another in nature |
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Definition
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Term
| When cloning a gene in bacteria, which of the following is the usual location of insertion of the new DNA? |
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Definition
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Term
| Restriction enzymes are enzymes that naturally occur in your body, which break up pieces of DNA from bacteria |
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Definition
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Term
| The transfer of genes from one species to another can only be done in a laboratory. There are no known cases of genes moving from one species to another in nature |
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Definition
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Term
| To find a particular gene of interest in a gene library, researchers use a short fragment of DNA that is complementary to the gene. This fragment is called a: |
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Definition
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Term
| PCR (Polymerase Chain Reaction) is a method used by living cells to make a large number of copies of a protein |
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Definition
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Term
| A collection of host cells (usually bacteria) that each contain fragments of an entire genome is called: |
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Definition
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Term
| When cloning a gene in bacteria, which of the following is the usual location of insertion of the new DNA? |
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Definition
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