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Ch. 10- Chemotrophic Energy Metabolism
Cell Biology
56
Biology
Undergraduate 3
10/16/2011

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Term
cellular respiration
Definition

the flow of electrons through or within a membrane, from reduced coenzymes to an external electron acceptor, usually accompanied by the generation of ATP

 

ATP yields much higher

Term
"external" electron acceptor
Definition
a type of electron acceptor that is not a by-product of glucose metabolism
Term
"internal" electron acceptor
Definition
a type of electron acceptor such as pyruvate or acetaldehyde that is used during fermentation to accept electrons from NADH and produces by-products that are not completely oxidized to CO2
Term
NADH
Definition
the reduced coenzyme generated by the glycolytic catabolism of sugars or related compounds
Term
FAD & Coenzyme Q
Definition
collect electrons that are removed from oxidizable organic substrates and pass them to the terminal electron acceptor via a series of electron carriers, generating ATP in the process
Term
aerobic respiration
Definition
respiration requiring oxygen or rare other electron acceptors such as Sulfur, Hydrogen protons, and Ferric ions (Fe)
Term
anaerobic respiration
Definition
respiration that involves electron acceptors and does not require oxygen
Term
role of mitochondria
Definition

most aerobic ATP production in eukaryotic cells takes place here

 

"energy powerhouse"

 

found in plants and animals

Term
terminal electron acceptor for aerobic respiration
Definition

oxygen-->pyruvate can be oxidized completely to carbon dioxide, thus allowing for the generation of up to 38 molecules of ATP per glucose

 

also provides a means for the continuous reoxidation of NADH and other reduced coenzymes

 

overall, aerobic respiration involves oxidative pathways in which electrons are removed from organic substrates and transferred to coenzyme carriers, which then transfer these electrons to oxygen, accompanied by the generation of ATP

Term
Stage 1: Aerobic Respiration
Definition
glycolytic pathway--> oxidation of glucose to pyruvate and then instead of serving as an electron acceptor, pyruvate is further oxidized
Term
Stage 2: Aerobic Respiration
Definition
pyruvate is oxidized to generate acetyl coA, which then enters the tricarboxylic acid (TCA) cyle
Term
Stage 3: Aerobic Respiration
Definition
the TCA cycle completely oxidizes acetyl coA to carbon dioxide and conserves most of the energy as high-energy reduced coenzyme molecules
Term
Stage 4: Aerobic Respiration
Definition
involves the transfer of electrons from reduced coenzymes to oxygen (electron transport),exergonic and uses energy to drive----> coupled with the pumping of protons across a membrane (active transport)
Term
Stage 5: Aerobic Respiration
Definition
the energy of the proton gradient is used to drive ATP synthesis in a process called oxidative phosphorylation (a process that synthesizes ATP from ADP)
Term
outer membrane of mitochondria
Definition
not a significant permeability barrier for ions and small molecules because it contains transmembrane channel proteins called porins that permit the passage of solutes
Term
inner membrane of mitochondria
Definition

presents a permeability barrier to most solutes, thereby partitioning the mitochondrion into two separate compartments--> the intermembrane space and the mitochondrial matrix

 

has distinctive inner folding called cristae that greatly increase surface area, because of this, it can accomodate large numbers of the protein complexes needed for electron transport and ATP synthesis

Term
semi-fluid matrix
Definition
contains many enzymes involved in mitochondrial function as well as DNA molecules and ribosomes
Term
mitochondrial genome
Definition
consists of a circular DNA molecule that code for ribosomal RNA's and about a dozen polypeptide subunits made of inner membrane proteins
Term
F1 complexes
Definition
complexes involved in ATP synthesis and is assembled from several different polypeptides
Term
Fo complex
Definition
each F1 complex is attached by a short protein stalk to this complex, which is assembled of hydrophobic polypeptides that are embedded within the mitochondria's inner membrane
Term
FoF1 complex
Definition

known as ATP synthase, responsible for most of the ATP generation that occurs in the mitochondria and bacterial cells

 

ATP generation driven by an electrochemical gradient of protons across the membrane

Term
respiratory functions of bacteria
Definition

bacteria do not have mitochondria, yet most are capable of aerobic respiration

 

most of the enzymes of the TCA cycle are found in the cytoplasm, whereas the electron transport proteins are located in the plasma membrane

Term
TCA cycle, or Krebs cycle
Definition
cyclic metabolic pathway that oxidizes acetyl CoA to carbon dioxide in the presence of oxygen (aerobic), generating ATP and the reduced coenzymes NADH and FADH2
Term
acetyl CoA
Definition
high-energy two carbon compound generated by glycolysis and fatty acid oxidation employed for transferring carbon atoms to the TCA cycle 
Term
Five general steps of the TCA cycle
Definition
READ page 262
Term
allosteric regulation
Definition

control of a reaction pathway by the effector-mediated reversible interconversion of the 2 forms of an allosteric enzyme

 

bahaha, what?

Term
fats as a source of energy
Definition

fats are important for long term energy storage

 

they are well suited for this storage function because they allow a maximum number of calories to be stored compactly

 

most stored as triacylglycerols

Term
Beta (B) oxidation
Definition
a catabolic process that generates acetyl CoA and the reduced coenzymes NADH and FADH2
Term
proteins as a source of energy and amino acids
Definition

proteins can be catabolized to generate ATP if necessary

 

all cells eventually undergo turnover of proteins and protein containing structures, and the resulting amino acids can either be used to synthesize new proteins or be degraded oxidatively to yield energy

Term
proteolysis
Definition

protein catabolism begins with hydrolysis of the peptide bonds that link amino acids together in the polypeptide chain

 

enzymes responsible are called proteases

Term
free amino acids
Definition
can also be catabolized for energy purposes
Term
amphibolic pathway
Definition
series of reactions that can function both in a catabolic mode and as a source of precursors for anabolic pathways
Term
glyoxylate cycle
Definition
similar to the TCA cycle, but performs specialized anabolic function in some germinating seeds and fungal spores, occurs in a peroxisome called glyoxysome
Term
electron transport
Definition
process of coenzyme reoxidation under aerobic conditions, involving stepwise transfer of electrons to oxygen by means of a series of electron carriers
Term
electron transport system, ETS
Definition
group of membrane bound electron carriers that transfer electrons from the coenzymes NADH and FADH2 to oxygen
Term
flavoproteins
Definition

used in the electron transport system

 

proteins that participate in electron transport via FAD or FMN

Term
iron-sulfur proteins
Definition

part of the electron transport system

 

proteins involved in mitochondrial transport

Term
cytochromes
Definition

five different kinds of proteins involved in the electron transport system

 

one-electron carriers that do not transfer protons

Term
bimetallic iron-copper center
Definition
cytochromes that have a copper center
Term
coenzyme Q
Definition
nonprotein (quinone) component of the mitochondrial electron transport system that serves as the collection point for electrons from both FMN- and FAD-linked dehydrogenases
Term
respiratory complex
Definition

subset of carriers of the electron transport system consisting of a distinctive assembly of polypeptides and prosthetic groups

 

unlike some integral proteins of the plasma membrane, the protein complexes of the mitochondrial inner membrane are free to diffuse within the membrane

Term
complex I
Definition
complex that transfers electrons from NADH to coenzyme Q
Term
complex II
Definition
complex that transfers to CoQ the enzymes derived from succinate
Term
complex III
Definition
complex that accpets electrons from CoQ and passed them to cytochrome c
Term
complex IV
Definition
complex that transfers electrons from cytochrome c to oxygen
Term
random side note
Definition
look at diagram 10-16 on page 275 for process including the 4 complexes
Term
Q cycle
Definition
proposed pathway for recycling electrons during mitochondrial or chloroplast electron transport to allow additional proton pumping across the membrane containing the electron carriers
Term
terminal oxidase
Definition
a component of complex IV that transfers electrons directly to oxygen
Term
oxidative phosphorylation
Definition
formation of ATP from ADP and inorganic phosphate by coupling the exergonic oxidation of reduced coenzyme molecules by oxygen to the phosphorylation of ADP
Term
electrochemical proton gradient
Definition
transmembrane gradient of an ion, with both an electrical commponent due to the charge separation quantified by the membrane potential and a concentration component
Term
respiratory control
Definition
regulation of oxidative phosphorylation and electron transport by the availability of ADP
Term
chemiosmotic coupling model
Definition
model postulating that electron transport pathways establish proton gradients across membranes and that the energy stored in such gradients can then be used to drive ATP synthesis
Term
proton translocator
Definition
Fo complex, the channel through which protons flow across the mitochondrial inner membrane
Term
ATP Synthase
Definition
FoF1 complex, provides a channel for the exergonic flow of protons through the membrane and carries out the synthesis of ATP
Term
binding change model
Definition
see page 282 figure
Term
electron shuttle system
Definition
model that consists of one or more electron carriers that can be reversibly reduced with transport proteins present in the membrane for both the oxidized and reduced forms of the carrier
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