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sum of all chemical rxns in an organism |
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-breakdown of complex organic compounds into simpler ones -releases energy (bonds broken) |
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-building of complex organic compounds from simpler ones -requires energy |
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substrate level phosphorylation |
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when a high energy Pi is directly transferred from a phosphorylated compound to ADP |
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oxidative phosphorylation |
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-electrons are transferred from organic compound to one group of electron carriers(NAD+ and FAD) -electrons are then passed through a series of different carriers to molecules of oxygen or other oxidized inorganic and organic molecules |
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-specific to photosynthetic organisms -electrons are released from chlorophyll by light and taken over by an ETC |
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2 processes to produce energy from glucose |
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-cellular respiration -fermentation |
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-starts with glycolysis -pyruvic acid is produced and processed in Krebs cycle and then ETC -total yield 38 ATP/glucose |
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pyruvic acid is converted into one or more different products such as ethanol, lactic acid, acetic acid, etc. -total yield 2 ATP/glucose |
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-final electron acceptor is oxygen yields 38 ATP |
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-final electron acceptor is inorganic molecule other than O2 (sometimes organic)
-NO3-, SO42-,CO32-
-always yields less than 38 ATP |
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alternate pathways to glycolysis |
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-pentose phosphate pathway -Entner-Doudoroff pathway -fermentation -lipid catabolism -protein catabolism |
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pentose phosphate pathway |
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-provides a way to breakdown 5-carbon sugars -produces a 5C sugar involved in the synthesis of nucleic acids, glucose from CO2 in photosynthesis and certain amino acids -ex. B. subtilis, E. coli, Enterococcus faecalis -Net gain 1ATP/glucose (vs 2 in glycolysis) |
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-allows to metabolize glucose w/o glycolysis or pentose phosphate pathway -net gain 1 ATP and 1 NADPH -ex. rhizobium, pseudosomonas, agrobacterium |
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-releases energy from sugars or other organic molecules (amino acids, purines, pyrimidines) -doesnt require oxygen -organic molecule as e- acceptor -1-2 ATP produced (only during glycolysis) |
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-lipids -glycerol-glycolysis-pyruvic acid-acetyl CoA-Krebs -fatty acid-Acetyl CoA-Krebs |
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-protein broke down by pepsidase or protease -deamination, decarboylation, or dehydrogenation |
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biochemical tests based on microbial metabolism |
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-detection of amino acid catabolyzing enzymes -fermentation test for detection of acid and gas production (durham tube) -peptone agar containing ferric acid-if bacteria produce H2S, it comebines with Fe to form FeS |
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-plants, algae, cyanobacteria -purple sulfur and green sulfur bacteria |
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-light energy is used to convert ADP to ATP by photophosphorylation -conversion of NADP+ to NADPH -in plants, algae, and cyanobacteria, chlorophyll a absorbs energy from light -purple and green sulfur bacteria use bacteriochlorophylls -cyclic and noncyclic |
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-Calvin Benson cycle -Co2 is fixed and used to synthesize sugars |
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why fermentation takes place in the absence of oxygen |
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-means to restore electron carrier NADH to oxidized state so it can re-enter glycolysis |
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