Term
| Molecular motors are controlled by what filaments? |
|
Definition
|
|
Term
Acrylamide in your food?
“…acrylamide in food is formed when …___ reacts with certain naturally occurring sugars such as ____. This only happens when the temperature during cooking is sufficiently high, a temperature which varies depending on the properties of the product and the method of cooking…” |
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
ATP hydrolysis
Binds to filament
Called motor domain |
|
|
Term
|
Definition
Determines the cargo that is bound
Determines the biological function of motor |
|
|
Term
| Kinesins and myosins both |
|
Definition
Structurally similar
Bind and hydrolyze ATP to facilitate movement
Changes in structure associated with ATP hydrolysis
This conformational change in structure results in movement |
|
|
Term
|
Definition
Globular N-terminal head
Binds and hydrolyzes ATP to move along actin filaments
Walks to +
All but one of the myosin family of motors go to + end (VI) |
|
|
Term
|
Definition
Globular head domains
Movement along microtubules toward + end
Humans have >40 |
|
|
Term
Myosin II think filaments
Myosins are bound together by their ____ into thick filaments
Thick filament is ____
No ______ between the two heads of the myosin
Myosin does not move processively, it only takes____ before letting go |
|
Definition
| tails, bipolar (motors face both ways), , coordination, one or a couple of steps |
|
|
Term
|
Definition
Brain sends signal
Motor neuron triggers an action potential at muscle cell membrane
Results in release of calcium from sarcoplasmic reticulum |
|
|
Term
Myofibrils contain
Thin and thick filaments |
|
Definition
|
|
Term
Thick filaments
Thin filaments |
|
Definition
|
|
Term
What does calcium do?
Myofibril - made up of ____ - has ___
Actin has other molecules bound - _____
Resting state - ____ binds to ______ and pulls it out of the groove - inhibits binding to____ (thick filaments)
Calcium releases ... |
|
Definition
sarcomere, actin
tropomyosin and troponin
troponin, tropomyosin
myosin II
tropomyosin from troponin |
|
|
Term
Sliding filaments
Thick filament bipolar -_____ moves to _____ end of actin filament |
|
Definition
|
|
Term
| Calcium release causes _____ and calcium ______ into the sarcoplasmic reticulum by _______ leads to ______ - both require a lot of ATP |
|
Definition
| contraction, reuptake, Calcium ATPase, relaxation |
|
|
Term
|
Definition
Myosin bound in non-nucleotide bound form (RIGOR)
ATP binding causes release of filament |
|
|
Term
| Hydrolysis to ______ associated with the myosin head moving forward |
|
Definition
|
|
Term
| Release of ___________; now the head snaps back to the original position - called the ______. |
|
Definition
Pi and tight binding of myosin-ADP
POWER STROKE |
|
|
Term
| After the _____ (conformational change), |
|
Definition
| Power Stroke, ADP is released, returning the Myosin head back to original configuration. |
|
|
Term
Kinesin motor movement
Start with...
ATP binding to forward head causes head to be ____ and also causes conformational change so that the other head ... |
|
Definition
...one head bound and one head not bound.
...tightly bound (rigor) to microtubule
...swings past the ATP-bound head |
|
|
Term
| Note the difference - rigor is ATP-bound for____ as compared to non-nucleotide bound for_____ |
|
Definition
|
|
Term
Kinesin motor movement continued...
______from the front head - now the non-nucleotide bound head binds to _____
And ATP is hydrolyzed (___________)
.... are coupled to create movement of arms |
|
Definition
ADP is released, microtubule
back to the beginning
ATP hydrolysis and ATP binding |
|
|
Term
The time that any given kinesin head is attached is ...
The time that any given myosin head is attached is... |
|
Definition
half the time
very little of the time |
|
|
Term
Kinesins v. Myosin
Kinesins move .... (this has to do with the fact that it is always bound by one or the other head)
Processivity allows ...from one place to another in cells - myosin never operates ... |
|
Definition
...processively - meaning that travel for hundreds of cycles without dissociating
...very few motors to move organelles
...as a single molecule (always an array) |
|
|
Term
Myosin v. Actin
Movement is based on ...
Speed is gained by ... kinesins |
|
Definition
...attachment and rate of ATP hydrolysis
...myosin being in arrays compared to |
|
|
Term
|
Definition
Organelle placement (Golgi) (tug-of-war)
Mitotic and meiotic spindle formation and chromosome separation |
|
|
Term
Kinesin Centripetal -
Kenesin Centrifugal - |
|
Definition
toward middle or center
toward periphery |
|
|
Term
|
Definition
| Attachment to membranes mediated by large protein complexes |
|
|
Term
|
Definition
| Tails bind to receptors for motor proteins on organelles |
|
|
Term
|
Definition
Cytoplasmic
Ciliary or axonemal (cilia and flagella) |
|
|
Term
| Dyenins are what type of motors |
|
Definition
Microtubule minus-end motors
Largest of motors and fastest |
|
|
Term
| Lysosome placement is dependent on ... |
|
Definition
|
|
Term
Alzheimer’s disease
______ disease
Characterized by ______ tangles and plaques
__________ - receptor for a motor protein (kinesin 1) in neurons
Defect in APP causes neurons to become clogged
Trafficking defect |
|
Definition
Neurodegenerative
neurofibrillary
Amyloid precursor protein |
|
|
Term
| Regulation of motor proteins |
|
Definition
Continuous tug of war on microtubules
Animals that can change color rapidly - response to hormone changes
Some pigment molecules transported on microtubules |
|
|
Term
| Phosphorylation of _____ light change releases from ______(____takes over) |
|
Definition
| kinesin, microtubule, dyenin |
|
|
