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Ch 12
Molecular Biology of the Gene
60
Biology
Undergraduate 1
05/03/2011

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Cards

Term
Any gene in the body contain the instructions for how what is made?
Definition
Polypeptide(protein)
Term
What is the conclusion from Avery et al's experiment?
Definition
DNA from the heat-killed S strain moved into the R strain causing the R strain to make a capsule
Term
How many strands of nucleotides does tRNA have?
Definition
1
Term
In a prokaryotic cell where does transcription occur?
Definition
Cytoplasm
Term
To begin transcription RNA polymerase binds to what region on the DNA so that the helix unwinds?
Definition
Prometer
Term
During RNA transcript processing the tail contains many nucleotides containing what base?
Definition
A
Term
Which portion of the mRNA transcript does the ribosome attach initially?
Definition
Cap
Term
If the finished mRNA transcript has a total of 435 nucleotides, how many amino acids join together?
Definition
144
Term
Which binding site on the ribosome always accepts a tRNA carrying a new amino acid to the construction site?
Definition
A site
Term
What prime carbon on an existing nucleotide add a phosphate of a new nucleotide?
Definition
5'
Term
If C is 21% in a cell what is the percentage of T?
Definition
29%
Term
According to Chargaff the % of A + ? =50%
Definition
G
Term
DNA can ONLY be synthesized in what direction?
Definition
5' to 3'
Term
One gene is the code for one what?
Definition
Polypeptide
Term
How many strands of DNA are used to make RNA during transcription?
Definition
1
Term
Which bonds to or is part of a tRNA?
Definition
Amino Acids, Anticodon, Codon
Term
During translation initiation the tRNA with what anticodon will pair with the start codon of mRNA?
Definition
AUG
Term
Before any new DNA nucleotides can be added to any daughter strand, what must be laid down as a starting point of the new daughter strand?
Definition
RNA Primer
Term
What enzyme adds DNA nucleotides to the daughter strand?
Definition
DNA polymerase
Term
What happens during Transcription?
Definition
DNA is copied to make one of 3 types of RNA
Term
Where does mRNA processing occur in the cell?
Definition
Nucleus
Term
Where does translation occur in a Eukaryotic cell?
Definition
Cytoplasme
Term
What joins together during the process of translation to make a polypeptide?
Definition
Amino Acids
Term
What binding site is a tRNA that currently has the existing polypeptide attached at?
Definition
P site
Term
An incoming tRNA carrying one amino acid would bind to which binding site?
Definition
A site
Term
How is the E site involved?
Definition
It release the tRNA's from the ribosome. It catches empty tRNA's
Term
What did Frederick Griffith study?
Definition
Stretococcus
Term
Which strain has a capsule around the plasma membrane?
Definition
S strain
Term
Which strain has rough appearance?
Definition
R strain
Term
What technique did Rosalind Franklin use to figure out structure of DNA?
Definition
Xrays
Term
What shape is DNA often referred to as having?
Definition
Double Helix
Term
Watson and Crick did what to win the Nobel Prize?
Definition
Build a model of DNA
Term
How many carbon atoms are in one deoxyribose sugar?
Definition
5
Term
The base is joined to the deoxyribose sugar at which carbon atom?
Definition
1'
Term
The phosphate is joined to the deoxyribose sugar at which carbon atom?
Definition
5'
Term
How many strands of nucleotides are in DNA?
Definition
2
Term
What bond joins the complementary bases together?
Definition
Hydrogen bonds
Term
In what phase of the cell cycle does DNA replicate?
Definition
S phase
Term
What enzyme synthesizes the new daughter strands based on the original parent strand's template?
Definition
DNA polymerase
Term
What enzyme separates the parent DNA by separating the Hydrogen bonds between the bases?
Definition
Helicase
Term
What do we call the daughter strand that is being synthesized grows continuously toward the replication fork?
Definition
Leading Daughter strand
Term
What fragments are formed when a daughter strand goes away from the fork?
Definition
Okazaki fragments
Term
The daughter strand that is synthesized grows dicontinuously away from the replication fork is called?
Definition
Lagging Daughter Strand
Term
Once replication is complete which enzyme removes the RNA primers and replaces them with DNA nucleotides?
Definition
DNA polymerase
Term
What enzyme joins the Okazaki fragments together?
Definition
DNA ligase
Term
What is the function of mRNA?
Definition
Takes messages from DNA in nucleus to Ribosomes in cytoplasm
Term
What is the function of tRNA?
Definition
Transfers amino acids to ribosomes
Term
What is the function of rRNA?
Definition
They make up the ribosomes
Term
What are the 2 major steps in making proteins using DNA instructions?
Definition
Transcription and Translation
Term
During transcription which molecule is made from the DNA template?
Definition
RNA
Term
During Translation, the order to nucleotides will determine the order of what ?
Definition
Amino acids
Term
How many nucleotides are in one codon?
Definition
20
Term
Each codon is the code for what?
Definition
Genetic Code
Term
What is the start codon?
Definition
AUG
Term
What are the stop codons?
Definition
UAA, UGA, UAG
Term
During initiation what binding site is tRNA at?
Definition
P site
Term
Elongation of transcription means that what is being formed gets longer and longer?
Definition
mRNA
Term
Termination of transcription occurs when the RNA polymerase comes to a DNA ___?
Definition
Stop sequence
Term
What is added at the 3' end of the mRNA transcript?
Definition
A tail
Term
What is taken out of the pre mRNA?
Definition
Introns
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