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ATP-producing catabolic pathway in which both electron donor and acceptor are organic compounds. Can be anaerobic. Results in partial degradation of sugars. |
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Combustion of Methane (Equation) |
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CH4 + 2O2 > CO2+ 2H2O + Energy |
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Nicotinamide Adenine Dinucleotide (NAD+) |
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Electron acceptor (oxidizing agent) that functions as a coenzyme |
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enzyme that catalyzes NAD+ reduction by removing 2H, delivering 2e- and 2p+, and releasing 1p+ |
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In the ETC, ATP synthesis is powered by |
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Oxidative Phosphorylation |
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inner mitochondrial membrane |
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The final e- acceptor in the ETC |
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oxygen (highest electronegativity) |
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Glycolysis takes place in the |
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Net reactions of glycolysis |
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Glucose > 2P 2ADP + 2P > 2ATP 2NAD+ > 2NADH + 2P+ |
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Energy-investment phase: the cell uses ATP to phosphorylate the intermediates of glycolysis. Energy-yielding phase: two 3-carbon sugars are oxidized |
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Substrate-level phosphorylation |
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ATP production by direct enzymatic transfer of phosphate from an intermediate substrate in catabolism. 2 produced in glycolysis, 2 in the Krebs cycle |
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Between Glycolysis and the Krebs Cycle |
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1. Pyruvate is transfered into the mitochondrion by facilitated diffusion 2. A multi-enzyme complex removes CO2, oxidizes the 2-C fragment into acetate, reduces NAD+ to NADH, and attaches CoA to the acetyl group. The bond is unstable and therefore reactive. |
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Krebs Cycle occurs in the |
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remaining acetyl fragments of acetyl CoA into CO2 |
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1 mol NADH > 1 mol FADH2 > |
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NADH and FADH2 pass e-s down electronegativity gradient to O: 2 NADH > 1 O2 reduced to 2 H2O, FADH2 lower Generates proton gradient across inner membrane that stores potential energy that can be used to form ATP |
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mechanism for coupling exergonic e- flow down an ETC tot he endergonic process of ATP production by creating proton gradient across the membrane Coupling between chemical and transport processes (phosphorylation and proton transport) |
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enzyme that makes ATP by using the proton gradient |
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ETC creates a proton gradient by |
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pumping H+ from the mitochondrial matrix across the inner membrane to the intermembrane space maintainable because inner membrane is impermeable to H+ |
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potential energy stored in proton gradient |
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Max. ATP produced in eukaryotic cells |
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