Term
| Differential centrifugation |
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Definition
separation based on differences in g-force needed to pellet particles
-rapid, easy |
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Term
| (Equilibrium) Density gradient centrifugation |
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Definition
separation based on buoyant density
gradient material sucrose and CsCl2
-do not use if you want to separate intact organelles |
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Term
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Definition
anchored into the bilayer, but sit on the surface
-extracted by using high salt or a solution with a pH of 11 |
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Term
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Definition
go all the way through the bilayer
-extracted by detergents |
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Term
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Definition
| transport of one substance against the concentration gradient is coupled to down gradient transport of another molecule |
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Term
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Definition
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Term
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Definition
| charged macromolecules in cells attract counterparts, so therefore there is an excess of ions this excess causes water to enter the cell |
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Term
| Defenses against the Donnan effect |
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Definition
plants,bacteria, and fungi-rigid cell wall counteracts the the pressure caused by osmosis
animals- pumps mainly Na/K-ATPases
protozoa-contractile vacuoles squeeze water out |
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