Term
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Definition
| A combination of cloned complementary DNA fragments inserted into a collection of host cells and stored as a 'library'. Lacks introns and can be readily expressed in a bacterial cell. |
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Term
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Definition
| Restriction endonucleases, ligases stick back together at sticky ends (or blunt ends) |
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Term
| How would you monitor to see if you have successfuly applied restriction enzymes and joined a fragment of DNA into a target strand? |
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Definition
| Run a gel electrophoresis |
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Term
| Does DNA move through electrophoresis gel towards the positive or negative electrode? |
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Definition
| Positive, DNA has a negative charge |
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Term
| What is the difference between sticky ends and blunt ends? |
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Definition
| Sticky ends will only join together with complimentary base pairs, whereas all blunt ends are compatible |
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Term
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Definition
| Self-replicating, small circular extrachromosomal DNA molecules |
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Term
| How would you go about making a cDNA library? |
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Definition
Extract mRNAs from the cell
Use reverse transcriptase to get the DNA
Use DNA polymerase to replicate the DNA
Put it into plasmids via restriction endonuclease/ligase |
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Term
| What are the three stages of PCR? |
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Definition
Denaturation
Annealing of primers
Extension of DNA |
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Term
| What is the main risk of PCR? |
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Definition
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