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Botany, Exam 1 Material
terms
103
Biology
Undergraduate 2
09/21/2013

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Term
protoplast
Definition
everything in the cell but the cell wall
Term
plasma membrane
Definition
covers surface of protoplast; is selectively permeable

is continuous through plasmodesmata
Term
nucleus
Definition
genetic info storage 'warehouse'

has nuclear envelope
Term
vacuole
Definition
storage containers; contain mostly water and salt, but can also store nutrients and cell waste (can act as toxin, advantageous)

store shit, digest shit, and can defend by nature of being toxic

analogous to lysosome in animal cells
Term
cytoplasm
Definition
protoplast - nucleus & vacuole
Term
mitocondria
Definition
carries out cellular respiration, taking HIGHLY ENERGETIC BUT NONREACTIVE compounds and turning them into reactive compounds that can be used (EX: ATP)

has own DNA and ribosomes
Term
ribosomes
Definition
synthesize proteins

(plant cells don't do this much compared to animals)
Term
ER
Definition
transport network of tubes and sheets that carries proteins and other things..

is continuous through plasmodesmata

is continuous with nuclear envelope
Term
rough ER
Definition
area of the ER that contains lots of ribosomes
Term
dictyosome
Definition
alters proteins synthesized in a ribosome if needed
Term
smooth ER
Definition
lacks ribosomes

synthesizes lipids and assembles membranes
Term
golgi body
Definition
cup-shaped congregation of dicyosomes; rare in plants (root hairs)

important for preparing secretory vesicles and glycosylation of proteins

site of synthesis of cell wall plysacharides
Term
endomembrane system
Definition
all membranes of the cell, except inner mitoconria and plastid membranes
Term
microbodies
Definition
not heavily researched.

peroxisomes and glyoxysomes
Term
cytosol or cytoskeleton
Definition
most of the volume of the cytoplasm

consists of mostly water, enzymes, and other things, notably actin microfibers and microtubules.

microtubules are most abundant and critical structural element of cells

centriole is 9 sets of 3 short microtubules

microfilaments are composed of actin
Term
cell wall
Definition
The primary cell wall is capable of rapid expansion during growth.

The secondary cell wall is deposited within the primary cell wall after
the cell reaches mature size.
Term
cellulose
Definition
a polysaccharide composed of 1,4-linked
β
-D-glucose
residues. Synthesized in the plasma membrane by cellulose synthase.
Term
hemicellulose
Definition
branched polysaccharides that are structurally
homolgous to cellulose because they have a backbone composed of
1,4-linked
β
-D-hexosyl
residues (mannose, galactose, xylose, etc).
Synthesized in the Golgi.
Term
pectin
Definition
a family of complex polysaccharides that all contain 1,4-linked
α
-D-galacturonic acid
. Synthesized in the Golgi.
Term
structural proteins
Definition
hydroxyproline-rich glycoproteins (HRGPs),
proline rich proteins (PRPs) and glycine-rich proteins (GPGs).
Synthesized at rough endoplasmic reticulum (RER), modified
in the Golgi.
Term
Main differences
between plant and
animal cells:
Definition
Plant cells have: cell
walls, a large central
vacuole, plastids and
turgor pressure.

Animal cells have a
lysosome (related to
vacuole) and centrioles
(function in organizing
microtubules).
Term
Cellulose synthase
Definition
an integral
membrane protein
that uses cytosolic
UDP-glucose as a
substrate to
synthesize
cellulose that it
deposits
extracellularly.
Term
Invertase:
Definition
Invertase: enzyme that catalyzes the hydrolysis of sucrose
Term
Sucrose
Definition
a disaccharide composed of glucose and fructose
Term
main differences between monocot and eudicot
Definition
Seed leaves: 1 for mono, 2 for euDIcot

pedals: 3 (or multiples of 3) for mono, 4, 5 for eudicot

circulatory system (vascular bundles): random for monocots, circular for eudicot
Term
3 main primary meristems
Definition
protoderm, procambium, and ground meristem
Term
protoderm
Definition
makes epidermis
Term
procambium
Definition
makes xylem and phloem
Term
ground meristem
Definition
makes pith and cortex
Term
3 main types of plant cells
Definition
parenchyma, collenchyma, and sclerenchyma
Term
parenchyma cells
Definition
uniformly thin, make up pith, epidermis, etc. alive at maturity
Term
collenchyma
Definition
unevenly thickened, for support. alive at maturity.
Term
sclerenchyma
Definition
adds a secondary wall composed of lignin. dead at maturity. ex: xylem
Term
2 meristems involved in secondary growth
Definition
vascular cambium and cork cambium
Term
vascular cambium
Definition
secondary xylem and phloem
Term
cork cambium
Definition
cork, bark, epidermis
Term
heartwood
Definition
no longer conducting water
Term
branch growth estimated by..
Definition
length between nodes
Term
lenicel
Definition
method of delivering O2 for gas exchange & respiration
Term
4 main steps in respiration
Definition
mobilization, glycolysis, TCA or anaerobic fermentation, electron transport & aerobic phosphorylation
Term
mobilization
Definition
produces GLUCOSE from STARCH

occurs in the STROMA
Term
glycolysis
Definition
occurs in cytoplasm

no carbon released

20% of energy available released

pyruvate main product. if O2 present, Krebs. if no, anaerobic fermentation (to recycle NAD+)
Term
in TCA/Krebs/Citric Acid cycle
Definition
C released as CO2

products: NADH & FADH

occurs in mitocondrial matrix (stroma)
Term
electron transport chain
Definition
occurs in inner membrane of mitocondria

protons pumped into inner membrane space, flow back to produce ATP

NADH & FADH can provide electrons

O2 is terminal acceptor of protons and water is formed
Term
ATP synthase
Definition
protons go into the matrix to produce atp
Term
autotroph
Definition
makes its own food

organism that makes its own food (organic molecules) from
inorganic molecules
Term
Tree of life: three Domains:
Definition
Eukarya (contains animals and plants), Archaea, and Bacteria
Term
prokayotic vs eukaryotic
Definition
plants are made of eukaryotic cells, which are much larger than prokaryotic cells
Term
Characteristics of plants
Definition
1.Autotrophic
CO2 + light -> glucose

2.Celluose cell walls
cellulose is a β1,4 linked glucose polymer(starch is α1,4 glucose; glycogen is branched 1,4 glucose)
-fungi have chitin (polymer of glucosamine) cell walls
-bacterial cell walls are peptidoglycan
-diatoms have a wall composed of silicic acid

3.Generally non motile

4.Gametes in multicellular gametangia

5.Most have well-defined alternation of generations (gametophyte
and sporophyte generations)
Term
invertase reaction
Definition
sucrose is α1, β2 linked glucose and fructose
Term
Cellulose and starch
Definition
cellulose is β1,4 linked glucose

starch is α1,4 linked glucose

they are both POLYMERS of glucose
Term
[4] Cell wall components
Definition
CELLULOSE
- a polysaccharide composed of 1,4-linked
β
-D-glucose
residues. Synthesized in the plasma membrane by cellulose synthase.

HEMICELLULOSE
- branched polysaccharides that are structurally
homolgous to cellulose because they have a backbone composed of
1,4-linked
β
-D-hexosyl
residues (mannose, galactose, xylose, etc).
Synthesized in the Golgi.

PECTIN
- a family of complex polysaccharides that all contain 1,4-linked
α
-D-galacturonic acid
. Synthesized in the Golgi.

STRUCTURAL PROTEINS
: hydroxyproline-rich glycoproteins (HRGPs),
proline rich proteins (PRPs) and glycine-rich proteins (GPGs).
Synthesized at rough endoplasmic reticulum (RER), modified
in the Golgi.
Term
where is cellulose synthesized? by what?
Definition
at the plasma membrane

by cellulose synthase
Term
exocytosis
Definition
fusion of a vesicle with the cell membrane, releasing the contents
of the vesicle to the cell exterior.

Other cell wall components such as hemicellulose and pectin are synthesized in the Golgi and delivered to the cell wall by
exocytosis
Term
plasmodesmata
Definition
connections between cells

connect the cytoplasm of adjacent cells,
the continuous cytoplasm is called the
symplast

The space outside of cells is called the
apoplast or cell wall space.
Term
cytoskeleton motor proteins
Definition
myosimn - moves on actin microfibers

Kinesin and Dynein motor proteins
move in different directions on
microtubules.
Term
Mitochondria and chloroplast
Definition
they're both semi-autonomous

they both divide by fission

both contain circular chromosomal DNA, located in nucleoids within the stroma/matrix.

- they contain ribosomes, tRNAs, etc.
- they depend on the import of nuclear encoded proteins for many functions (but can synthesize their own non-encoded protein)

Chloroplasts: function in photosynthesis: light reactions, dark reactions.
Starch synthesis and storage, chlorophyll and fatty acid synthesis.

Mitochondria: Main function is respiration (conversion of energy stored in
sugars to energy stored in ATP).
Term
endoreduplication
Definition
(occurs in nucleus)

DNA duplication is not followed by mitosis.

leads to an increased chromosome copy number and usually large cell size.
Term
Mitosis:
Definition
nuclear division
Term
Cytokinesis:
Definition
division of the protoplast
Term
cell cycle
Definition
Interphase (G1 S (chromosomes duplicated) G2)

Mitosis

Cytokinesis

notes:
control points before DNA synthesis and mitosis

preprophase band is part of cytoskeleton
Term
Mitosis
Definition
Prophase

Metaphase

Anaphase

Telophase
Term
Prophase
Definition
chromosomes condense,
chromatids held together by centromere
preprophase band disappears
-band marks plane of cell division
-is composed of microtubules
-is located in cytoplasm

Late prophase:
nuclear envelope disappears,
spindle develops
Term
Metaphase
Definition
chromosomes align on the equatorial
plane of cell division. The spindle apparatus
(microtubules) is assembled and attached to the
kinetochores (containing motor proteins).
Term
Anaphase
Definition
chromatids move on the spindle fibers
toward the poles.
Term
Telophase
Definition
Nuclear envelope reforms.
Cell plate forms between the separated
nuclei. This starts by the formation of a phragmoplast
that will form the new cell wall.
Term
phragmoplast
Definition
structure that becomes the new cell wall during cytokinesis

a complex of microtubules, actin microfilaments and Golgi-
derived vesicles.

A preprophase band of microtubules forms before prophase that marks the plane of cell division it dissapears in anaphase, in telophase a phragmoplast begins to form and will become the new cell wall.
Term
haploid
Definition
containing only one copy of each
gene
Term
zygote
Definition
the first diploid cell

the product of fertilization

New diploid cells are produced through mitosis and cytokinesis
Term
meiosis
Definition
two divisions without an intervening S phase. In metaphase I,
chromosomes are separated, not chromatids as in mitosis. The nuclei in telophase I are haploid.

Four haploid cells result from each cell that goes through meiosis.
Term
meristem
Definition
site in the plant body where new cells form and growth and differentiation is initiated
Term
Growth
Definition
an irreversible increase in size that comes from cell division and cell enlargement.
Term
cell differentiation
Definition
he development of a specialized cell, a cell with specialized structure, biochemistry etc. important for its function.
Term
the primary meristems in the SAM (shoot apical meristem)
Definition
Protoderm - epidermis

Procambium - primary xylem and phloem

Ground meristem - pith and cortex
Term
Parenchyma cells
Definition
spherical cells, generally the biochemical factories of plants.

Examples are the cortex and pith of stems and the mesophyll of leaves. Usually only have primary cell walls that remain thin.
Term
Collenchyma cells
Definition
functions in support in young stems and petioles.

Primary cell wall is unevenly thickened.
Term
Sclerenchyma cells
Definition
two types: fibers and sclereids.

They have thickened secondary cell walls.

They are dead at maturity.
Term
Epidermis
Definition
the outer cell layer, generally contains no chloroplasts, outer wall impregnated with cutin to inhibit evaporation.

Cell types: epidermal cells, guard cells,
subsidiary cells, trichomes
Term
guard cells
Definition
pairs of guard cells form stomatal pores in the epidermis and control the aperture (opening) of the pore.

The cells are guard cells the pore is the stomatal pore.


Note: guard cells do not open and close, they open and close the pore.
Term
subsidiary cell
Definition
type of epidermal cell that is in contact with guard cells. Subsidiary cells regulate guard cells. Not all plants have subsidiary cells.
Term
trichome
Definition
Elongated epidermal structure composed of one or more cells.

Functions in secretion, defense, storage, protection from high light.

They function in physical and chemical defense against herbivores such as insects.
Term
two types of vascular tissue and their functions
Definition
xylem - transports water and nutrients
from the roots to the rest of the plant.


phloem - transports sugars and metabolites from sources (usually photosynthetic leaves) to sinks (net importing tissue such as roots, developing leaves, fruit etc).

Transport in the phloem is driven by positive pressure.
Term
Sieve tube cells
Definition
living at maturity, lack many organelles such as nucleus, mitochondria, and vacuole.

In flowering plant sieve tubes are associated with companion cells.

They function in the long-distance transport of sugars and other metabolites. These are the main conducting elements of the phloem.
Term
Companion cells
Definition
Connected to sieve tubes by plasmodesmata.

They supply sieve tubes with proteins, ATP, etc.
Term
Tracheids and vessel elements
Definition
dead at maturity.

They contain lateral pits that conduct water and solutes.

Secondary cell walls fortify the structure to withstand strongly negative pressure potentials.

- Vessel elements have end wall perforations and form vessels.

[Xylem sap is continuous with surrounding cell wall space.]

[Gymnosperms only have tracheids.]
Term
Root hair cells
Definition
epidermal cells of the root with projections.

The function is to increase surface area for water and nutrient absorption.

Not every root epidermal cell becomes a root hair cell.

Note: root hairs are cellular extensions,lateral roots are multicellular.
Term
two mechanisms of cell growth
Definition
1) tip growth (root hairs, pollen tubes)


2) diffuse growth (almost all other cells).
Term
diffuse growth
Definition
orientation of cellulose microfibrils determines the polarity of cell expansion.

The orientation of cellulose is determined by interaction of cellulose synthase with microtubules.
Term
primary things..
Definition
Primary growth - Growth in vascular plants resulting from the
production of primary tissues by a primary meristem.

Primary meristem: Meristematic tissue in vascular plants derived from an
apical meristem, such as the procambium, protoderm, and ground
meristem.

Primary tissues: tissues derived from an apical meristem.
Term
Phyllotaxy
Definition
arrangement of leaves on the stem

Alternate: one leaf per node

Opposite: two leaves per node

Distichous: one leaf per node, arranged in only
two rows
Term
Vascular tissues
Definition
Primary phloem and xylem are always
found together in vascular bundles.

In stems and roots the phloem is oriented
to the outside.
Term
similarities and differences between xylem and phloem
Definition
Both are types of vascular tissue, both are found in vascular bundles, and both form tubes that conduct solutes for long distances in plants.

In the xylem the tracheids and vessel elements that form the conducting tubes are dead at maturity.
In the phloem the sieve elements that form the conducting tubes are living at maturity.

Transport in the xylem is driven by negative pressure. Transport in the phloem is driven by positive pressure.
Term
Turgor pressure
Definition
the positive hydrostatic pressure of a cell.

This pressure is generated by water accumulation driven by a negative solute potential.

The rigid cell wall prevents the cell from bursting.

Importance of turgor pressure: plant structure, cellular movement, cell growth, nutation (tissue movement).

A negative solute potential means that the cell accumulates solutes and that water is attracted and enters the cell by osmosis.
Term
How does turgor pressure affect guard cells?
Definition
Guard cells EXPAND under high turgor pressure (which makes sense; pores open, allowing for transpiration and CO2 entry)

Guard cells shrink under low turgor pressure (which makes sense; pores close because not wanting to loose any water under low water conditions)
Term
Modified stems
Definition
Bulbs – modified shoots,
underground stems surrounded by
modified fleshy leaves. Examples:
onion, daffodils, garlic. Function:
storage.

Corms – fleshy stems with
modified papery leaves.
Examples: gladiolus. Function:
storage.

Tubers – horizontal underground
fleshy stems. Example: potato, the
“eyes” are axillary buds. Function:
storage.

Rhizomes – horizontal underground stems that survive
harsh winters and allow plants to spread. Examples:
bamboo, irises, canna lillies,

Stolon – horizontal stem, also called a runner. Used to explore for suitable
habitat. Examples: strawberry, some grasses.
Term
photosynthesis
Definition
the conversion of CO2 into organic compounds using the energy of sunlight.

A thermodynamics definition: photosynthesis is the
conversion of light energy into chemical energy
(The first law of thermodynamics: energy can
change form but it cannot be created or destroyed.)

The biochemical definition Photosynthesis: the chemical formula

6CO2+ 6H2O + energy --> C6H12O6+ 6O2
Term
light reactions
Definition
occur on the thylakoid membrane

products: O2, ATP, and NADPH

The function of the light reactions is to generate ATP and NADPH

ADP + Pi -> ATP
NADP+ + H+ + 2e--> NADPH

In chloroplasts, the light reactions drive acidification of the thylakoid lumen via electron transport reactions.

The proton gradient is used by ATP synthase to generate ATP within the stroma.

Light also drives the reduction of NADP+ to NADPH in the stroma.

Other points of interest:
Water is the electron donor.
Oxygen evolved in photosynthesis comes from the oxidation of water.
NADP+is the terminal electron acceptor.
Term
carbon reactions
Definition
occur in the stroma

The function of the carbon reactions (used to be called the dark reactions)is to fix CO2 and regenerate ribulose 1,5 bisphosphate (RuBP).

This requires ATP and NADPH.
Term
Rubisco
Definition
enzyme that fixes carbon (also called ribulose 1,5 bisphosphate carboxylase/oxygenase; the most abundant
protein) in the stroma
Term
photorespiration
Definition
the recovery of carbon due to oxygenase activity of
Rubisco

occurs when Rubisco acts as an oxygenase rather than a carboxylase

When rubisco acts as an oxygenase the products are one 3-phosphoglycerate (C3) and one phosphoglycolate (C2).
Term
C4 and CAM plants
Definition
plants that have evolved strategies to avoid photorespiration.

These plants are specialized for growth at high temperatures and under dry conditions.

The purpose is to INCREASE THE CO2 CONCENTRATION in
chloroplasts where the Calvin cycle takes place.

In C4, the primary carboxylation (carbon fixation) is done by PEP carboxylase

The benefit of CAM photosynthesis is that stomata are open at night when water loss through evaporation is less.

However, it is expensive to store carbon as malate during the night, at least 2 ATP per CO2 fixed.
Term
Aquaporins
Definition
membrane proteins that transport water

they determine the rate of water movement in osmosis
Term
Heterophylly
Definition
different leaf shapes on the same plant.
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