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BOT 1-4
Exam 1
126
Biology
Undergraduate 1
02/21/2012

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Term
Compound Light Microscope
Definition
-Magnify up to 1000x
-Light passes through object to be viewed
-Clearly see organelles and cells as small as 2 microns.
Term
Dissecting Microscope
Definition
-Magnify up to 30x
-Used to view surface of object
Term
Transmission electron Microscope
Definition
-Uses electrons to visualize the sample
-Doesnt use white light
-Magnify more than 200k
-like compound light microscope
Term
Scanning Electron microscope
Definition
-Like dissecting microscope
-magnify up to 10k
Term
Taking out a microscope
Definition
1) Red lens in check
2) Stage back
3) Light off/ knob turned down
4) Clean
Term
Microscope parts
Definition
Base- light source
Condenser-acts like lens-allow light in
Mechanical stage
Objectives- 4x red
10x Yellow
40x blue
Term
Resolution
Definition
Closest 2 objects can be before they appear as 1.
Term
Mitorchondrion
Definition
FUNCTION:
-Site of aerobic cellular respiration
-Majority of AIP synthetics
BACTERIAL LIKE:
-Contains Proteins, DNA, RNA, Ribosomes
STRUCTURE:
-Double membrane
-Inner membrane highly folded
-folding=greater surface area= more ATP
BOUND MATRIX
Term
Vacuoles
Definition
-Membrane bound organelle
-Contains cell SAP
-Inorganic ions,sugars,organic acids, amino acids, pigments( anthocynan )
Term
Cytoskeleton
Definition
Network of protein filaments- act like mono rails.
-INVOLVED IN:
Cell growth division, differentiation
Organelle movement
Cytoplasmic streaming
Term
Cell Cycle
Definition
Interphase, mitosis, plant growth, wound repair
Term
Interphase
Definition
Chromosomes NOT visible
-Chromatin-no organization-all over
Divided up
-GL: long period
-S: Each chromosome is copied
Forms sister chromatids
-G2: Cells continue to ready to divide
Term
Mitosis
Definition
NUCLEAR DIVISION
Cell division
-daughter cells
-need to be genetically identical
-occurs in meristematic regions
Term
Cytokinisis
Definition
2 cells with individual nucleus
-provide all to create 2 complete cells
-DIVISION OF CYTOPLASM
Term
DNA
Definition
=CHROMATIN
Term
Prophase
Definition
CLEARLY SEE CHROMOSOMES
-chromosomes condense
-Chromatids visible
Centromere hold sister chroms together
-Membrane fragments
-Nucleolus disappears - disintegrates
Term
Metaphase
Definition
Chromatids line up at the metaphase plate
Term
Anaphase
Definition
Separation of sister chromatids
Term
Telophase
Definition
1)nucleus forms
2)chromatin forms
3)nucleol forms
4)cell plate forms
5)primary cell wall forms
6)middle llamelia
Term
Meristematic tissues
Definition
permanent points in plant where actively dividing

-mitosis
Term
Apical meristem (apical=tip or apex)
Definition
-A lot of cells in mitosis
-At or near the growing tips of shoot/roots
-Dividing cells produce "primary growth"
-produce primary meristem
Term
Primary meristem
Definition
Developed from meristem
procombrium- gives rise to
Term
Lateral Meristems
Definition
Grow in diameter
-increase the girth of roots+stems
-Secondary tissues
INCLUDE:
vascular cambium
-function=support and conduct
-conk cambium=produce bark and trees
Term
Intercalary meristems
Definition
(ONLY IN MONOCOT STEM)
-carcasses dont have vascular nor cork cambrium
-Apical meristem
--Nodes where leaves attach
--Add to stem length
Term
Parenchyma- simple tissue
Definition
FUNCTION: Food and storage tissue
-Ground tissues
-Most abundant cell type in plants
-Large Vacuoles
starch grains,oils,tanning
-Thin cell walls
-living cells = can divide
Term
Chlonencyma- simple tissue
Definition
-Parenchyma with chloroplasts
-photosynthetic tissue
-Recall elodea leaf
Term
Collenchyma- simple tissue
Definition
-Living
-thicker cell walls
-typically below epidermis
Term
Epidermis- complex tissue
Definition
-Outer most layer of cells
-secrete cutin
Forms cuticle, prevents evaporation, resists bacterial infection
-WAXES- ex. Bayberry candles, polishes
FORMATION OF ROOT HAIRS
Term
Periderm- complex tissue
Definition
-Outer bark of woody plants
-Cork cells
Dead
Cytoplasm is active
Secrete suberin
Term
Development
Definition
-Seed germination
radical-emerges 1st to feed and nourish
-Taproots
-Fibrous root system
-Adventitious roots ex. grass
Term
Mucilaginous-structure
Definition
allows to slide roots down and avoid abrasions

-in root cap
Term
Cell division
Definition
-apical meristem
-protoderm-epidermis
-ground meristem
Term
Root cap
Definition
-parenchyma cells
-protection
-gravitropism
Term
Elongation
Definition
Cells not dividing + cells elongating
-Grow wider
-vacuoles merge - forms large central vac
Term
Maturation
Definition
-Differentiate
-Root hairs
extentions of epidermal cells
-Increase absorption capacity
Term
Primary structure
Definition
3 tissues
-epidermis
-cortex
parenchyma=storage
-Vascular Cylinder
Endoderms
Term
Xylem - dicot root
Definition
Transports water from roots to aerial parts of plant trachids and vessels
Term
Phloem - dicot root
Definition
Transports food from aerial parts of the plant
Term
Pericycle - dicot root
Definition
Lateral root develop from this
Term
Monocot root
Definition
Multiple Primary xylem + phloem
Term
Specialized roots ( ADAPTATION )
Definition
ex. Yams, sweet potato, dandilions, carrots
Term
Mycoorhizae-Fungi
Definition
-Mutualistic-Cymbiosis-help each other out
-Aide in absorption w/ phosphr, nitr, water
-prevent disease
-energy & carbon consumption
Term
Root Nodules
Definition
-Bacteria
-legumes(ex. peas, beans, alfalfa)
-Convert atmospheric nitrogen into organic nitrogen
Term
Protoplasm
Definition
The living material of a cell
Term
Cytoplasm
Definition
Makes up protoplasm.
Main constituent is water

-contains organelles, membranes, particles, and dissolved substances.
Term
Ribosomes
Definition
Play a role in the manufacture of proteins
Term
Vacuoles
Definition
Occupy more than 95% of a plants cell volume.
Term
Cyclosis
Definition
Chloroplasts are carried along by a riverlike flow of almost invisible cytoplasm
Term
What is botany?
Definition
Study of plants
-Plant molecular biology
-plant biochemistry
-plant cell biology
-plant physiology
-plant anatomy
-plant genetics
etc...
Term
Scientific Method
Definition
Obervations
Questions
Hypothesis
-Tentative explanation
-testable
-falsifiable
Prediction
Experiment
Conclusion
Reiterations
Term
Treatment Groups - experiments
Definition
-subjects tested with what you are testing

-variable
Term
Control Groups - experiments
Definition
-subjects that you compare your treatment against
Term
Experimental Variable
Definition
(INDEPENDENT VARIABLE)
-manipulated variable
-plotted on x-axis of graph
Term
Response Variable
Definition
(DEPENDENT VARIABLE)
-outcome of experimental variable
-plotted on the y-axis of graph
Term
How are variables plotted?
Definition
|_ Wall depends on the floor
Term
How does life on earth depend on plants?
Definition
Oxygen, food, nutrients, shelter, clothes, medicine
Term
Common Elements
Definition
95%=macro nutrients

-carbon=food
-hydrogen=food+water
-oxygen
-nitrogen
Term
Less common elements
Definition
-Phosphorous
-sulfur
-potassium
Term
Covalent Bonds
Definition
Electrons shared
strong
non-polar covalent
-Electrons equally shared e.g CH4
polar covalent
-Electrons unequally shared e.g.H20
Term
Ionic bonds
Definition
-Electrons lost=CATION e.g. Na CATS:)
-Electrons gained=ANION e.g. Cl ANTS:(
-opposites attracted, NaCl
NO SHARING

lose 1 electron = positive charge
Term
Hydrogen Bonds
Definition
-Attraction between two polar molecules

e.g. 2 water molecules
Term
What is energy?
Definition
Capacity to do work/cause change
Term
Molecule
Definition
1 element (2 or more atoms)
Term
Compound
Definition
2 more more different elements
Term
What is an acid?
Definition
Positive H+ + Anion > Base
Term
What is a base?
Definition
NaOH > (Na+)+(OH-)

Drops ph = add hydroxide
Add ph = release hydroxide
Term
how to measure acidity
Definition
PH= -LOG(10)[H+] =10(-7) molar
PH=-log10(10(-7))
PH=(-)(-7)log(10)10
=1
PH=(-)(-7)(1)= (7)
Term
What forms can energy take?
Definition
Kinetic=Movement
Potential=Chemical bonds,Chemical gradients
Term
What is the 1st Law of Thermodynamics
Definition
Energy can neither be created nor destroyed it can though be transformed from one form to another.
Term
What is the 2nd Law of Thermodynamics?
Definition
For each transaction of energy, some energy is degraded to a less usable form

ex. Thermal energy=heat
Term
Monosaccharides (mono=single)
Definition
MONOMERS OF POLYMERS
=Building blocks

-(CH2O)n
-Glucose-C6 H12 O6
-Fructose-C6 H12 O6
-Ribose-C5 H10 O5
Term
Disaccharide (di=two)
Definition
-Sucrose
Transported sugar in plants

Composed of 2 monocaccharide
-glucose and fructose
Term
Dehydration Synthesis
Definition
-Condensation reation(pulling out)
Term
Degration
Definition
Hydrolysis(ADD WATER)
-Split molecule into 2 by adding H20

Hydrolytic=BREAK BOND
Term
Polysaccharide (poly=many)
Definition
Composed of monosacchrides or some dissacrides
Term
Starch
Definition
-Storage charbohydrate
-composed of glucose
Term
Cellulose (cows, termites can digest)
Definition
-Structural carbohydrate
-Composed of glucose
Term
LIPIDS-Fats & Oils
Definition
Function: Energy storage, structural, waterproofing

Chemically diverse
Hydrophobic(NONPOLAR)water hating
NOT FORMED FROM MONOMERS
Term
Neutral fats and oils
Definition
Composed of
-Glycerol backbone
-3 fatty acids
-dehydration synthesis
-hydrolysis degradation

HYDROPHOBIC (non-polar)
-Saturated or unsaturated
Term
Phospholipids
Definition
Composed of
-Glycerol
-2 fatty acids
-Phosphate group

Hydrophobic(nonpolar)/hydropholic(polar)

Lipid bilayer formation
-cellular membranes
Term
Function as barriers to water loss
Definition
Cutin, Suberin, and waxes
Term
Proteins
Definition
Extremely diverse
FUNCTIONS:
-Enzymes
-structural
-Storage
-Transporters
-Hormonal
-Receptors
-Defensive
Term
Amino acids
Definition
Monomers of proteins
20 different kinds
structure
-Amino group-NH2
-Acidic group-COOH
-Side group-R
Polar, non-polar, basic, acidic
Term
Protein structure
Definition
Polypeptide
Peptide bonds-dehydration reaction
3D shape- Depends on the sequence of amino acids in polypeptide

3D shape dictates functionality
Term
Enzymes
Definition
Protein
Catalyst
-Active sites
-Accelerates reations
Lowers activation energy
Term
Nucleic Acids
Definition
Functions:
-store DNA & RNA information
-Energy currency of a cell

-Polynucleotides
-Dehydration reaction between nucleotides to synthesize polynucleo
-Hydrolysis to degrade polynucleotid
Term
Nucleotides
Definition
Monomers of DNA & RNA
Composed of:
-ribose or deoxyribose
-Nitrogenous base
Adenine(DNA&RNA)
Thymine(DNA only)
Guanine(DNA&RNA)
Cytosine(DNA&RNA)
Uracil(RNA only)
Term
ATP
Definition
Cell's energy
-Adenosine Triphosphate
Energy currency
Cleave off a phosphate group
-Release useful energy (DO WORK)
-Release wasteful energy(RELEASE HEAT)
Term
Who contributed to the Cell Theory- all living things are composed of cells?
Definition
Robert Hooke in 1665
Term
Who described living microorganisms from teeth scrapings, rain water, and peppercorn infusions?
Definition
Anton Van Leeuwenhoek in 1673-1723
Term
Who published the Cell Theory?
Definition
Schleiden and Schwann in 1838
Term
Cell Theory
Definition
-All living things are composed of one or more cells
-Chemical reactions occur in a cell
-Cells arise from other cells
-Cells contain hereditary information which is passed on to offspring cells
Term
Prokaryotes-Type of cell
Definition
-No nuclear membrane-bound nucleus
-No organelles
-Bacteria and Archaea
Term
Eukaryotes-Type of cell
Definition
-Nuclear membrane-bound nucleus
-Organelle
-Plant, animal, protista, and fungi
Term
Cell wall
Definition
COMPOSED OF:
-Principally Cellulose
-Hemicelluloses
-Pectins
-Lignin
FUNCTIONS:
-Cell shape
-Prevent rupturing
-Role in cell physiology
-Role in pathogen resistance

MIDDLE LAMELLA:
-Composed of pectin
-Shared by adjacent cells
Term
Plasma membranes
Definition
Boundary between the inside and outside worlds of a cell

COMPOSITION:
-Phospholipids
-Proteins

LIPID BILAYER "Ocean"
PROTEINS "Ships"
Term
Nucleus
Definition
-Stores genetic info
-controls activity of cell
Term
Chloroplats
Definition
-Site of photosynthesis
-STRUCTURE:Double membrane, Thylakoid(green, converts light to energy)
Stacks of thylakoid mem=granum


CONTAINS DNA & RIBOSOMES
Term
Chromoplasts
Definition
Pigmented Plastid
-Carotenoids-yellow,red,orange pgmnt
-role of unknown nature, to attract insects?
Term
Leucoplasts
Definition
-Non-pigmented plastid
-Amyloplasts
Synthesize and store starch
LIGHT DEPRIVED CHLOROPLASTS=leucoplasts
Term
Where is cytoplasm located in most cells?
Definition
Inside the plasma membrane
Term
What are cytoplasmic bridges?
Definition
Thin strands of cytoplasm
Term
If present in a cell, where are anthocyanin pigments located?
Definition
in the cell vacuoles
Term
In what part of a cell are cholorplasts located?
Definition
Cytoplasm
Term
What is the thin boundary of the vacuole called?
Definition
Vacullar membrane
Term
Specifically where do starch grains develop in a cell?
Definition
within cell walls
Term
Where would you expect to find a nucleolus?
Definition
Nucleus
Term
how can you distinguish anthocyanin pigments and chromoplasts?
Definition
Bigger "blob"= annthoyanin
Term
What changes in a cell's cytoplasm and nucleus take place during interphase?
Definition
It prepares itself for the begining of mitosis.
Term
In which region of a root tip does mitosis occur?
Definition
Meristematic region
Term
What is a spindle?
Definition
Top-shaped fiber that extends netween 2 invisible opposite poles
Term
What are the main events of metaphase?
Definition
Centromes align at equator & spindle fibers completely develop.
Term
In which phase of mitosis do chromosomes first become distinguishable from one another?
Definition
Anaphase
Term
What happens to chromosomes during anaphase?
Definition
Strands separate lengthwise at centromes and one strand of each pair goes to opposite poles.
Term
Of what substance is a middle lamella composed?
Definition
Pectin
Term
What becomes of the cell plate at the conclusion of mitosis?
Definition
After growing outward and unites with plasma membrane. Cellulode is deposited on membrane resulting in new cells. NEW CELL WALLS.
Term
When do single-stranded chromosomes become two-stranded?
Definition
Prophase
Term
In which specific regions of a plant does mitosis commonly take place?
Definition
Meristematic & elongation
Term
In which phase of mitosis do nucleoli disappear?
Definition
Prophase
Term
When do nucleoli reappear?
Definition
Telophase
Term
What takes place in metaphase?
Definition
Chromosome centromeres align at equator, top shaped spindle is fully developed.
Term
Poles, equator, & spindle are all visable in...
Definition
at least 1 stage of mitosis.
Term
What occurs in anaphase?
Definition
Division of cells is obvious
Term
From what does a new cell wall develop?
Definition
Cellulose
Term
Which cell organelle produces the materials for a new middle lamella?
Definition
Pectilin is added to the cell membrane.
Term
What is the function of a centromere?
Definition
Acts as the point of mitotic spindle attachment.
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