Term
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Definition
ways to colonize and breach host surfaces:
-loss of barrier function
-adhesion molecules
-fimbrae, pili
-motility and chemotaxis
-proteases
-surface binding proteins
-bacterial capsules
-toxic proteins
-variability of surface antigens |
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Term
other virulence factors (2) |
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Definition
- evasion and resistance to antibacterial peptides
-iron acquisition compounds |
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Term
mechanisms of infectious diseases |
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Definition
- acquisition of disease
-asymptomatic phase
-prodromal phase
-symptomatic phase
-healing convalescence |
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Term
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Definition
-Nature of disease: tissue target, entry site, accessibility of virus to target tissue; tissue tropism; permissiveness of viral replication ; viral strain
-severity of disease
-cytopathicity
-health status of patient
-virus innoculum
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Term
cytopathicity:
3 potential outcomes of a viral infection |
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Definition
1-failed infection--no infection
2-lytic infection--cell death
3-persistent infection--chronic or latent or immortalizing |
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Term
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Definition
-herpes viridae family (HHV)
-herpes simplex virus (HSV 1&2)
-varicella zoster virus (VZV)
-epstein barr virus (EBV)
-cytomegalovirus (CMV)
-HHV 6,7,8 (exanthems) |
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Term
herpes virus characteristics
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Definition
-ubitquitous, highly infective, small, five sides figure
-ds DNA virus
-DNA envelope has 4basic components
-classified by virulence in cell structure
-viral envelope proteins bind to host cell plasma membrane receptors, enter the cytolasma via endocytosis, virons uncoat, and viral DNA enters host's nucleus
-replication will cause mental swelling |
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Term
basic characteristics of viruses |
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Definition
-lack their own metabolism!
-can replicate in both pro/eukaryotic cells
-contain 2 genomes
-coding for structural proteins
-non-coding proteins (enzymes such as RNAase and DNAase, and immune signaling proteins) |
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Term
how viruses work basically |
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Definition
attachment
penetration
uncoating of viral nucleic acid
transport of viron
synthesis of mRNA
replication of viral DNA
assembly
dispersion of viron out of cell (egress) |
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Term
recurrent herpes simplex infections
(7 "phases")
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Definition
-reactivated virus
-prodromal symptoms
-minimal systemic symptoms
-small group of vesicles (new lesions for 1-2 days)
-viral shedding(3-5 days)
-crusting of vesicles
-healing (9-10 days) |
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Term
how a virus may get reactivated
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Definition
-virus hangs out in neural ganglion
-during periods of stress or compromised immune system the virus travels down teh axons like a slinky
-LAT (latency associated transcriptase) keeps virus dormant--when this gene turns off you get prodromal symptoms which are mostly neural (tingly lips, etc.)
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Term
primary course of HSV infection |
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Definition
-incubation of virus for about 5 days
~day "0" pt experiences systemic and local symptoms
-systemic--> tender lymph nodes
-local->pain/itching/dysuria/malaise/fever/headache
~day 2-5 you see more systemic symptoms
-vesicle, wet ulcer, crusting
-shedding virus for ten days or so!
~day 15 you begin healing |
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Term
recurrent course of HSV infection |
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Definition
-prodromal symptoms for 1-2 days before day "0"
-day "0" get lesions & local symptoms; shedding virus
~day 10, start to heal |
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Term
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Definition
-mostly pallative
-hydration
-antipyretics
-prophylactic tx with acyclovir sometimes
-affects immediate early protein gene transcription and/or early protein phase gene transcription
*thus inhibits DNA elongation/synthesis |
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Term
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Definition
-varicella-->chicken pox in kids
-longer incubation period (14-17); prodromal rare
-lesions develop over 3 days
-itchy rash persists for about 1-6 days
-crusting and shedding for about 7 days
-fever 101-105 degreese
-no live attentuated oka vaccine
-zoster-->virus reactivated to cause shingles |
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Term
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Definition
-prodrome symptoms common for 4-5 days
-vesicles develop withint 12-24 hrs and rash persists for 1-7 days
-rash forms "belt" or follows CN V1,V2,V3 on face
-pustules dry and crust and fall off |
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Term
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Definition
-causes mononucleosis
-tx: supportive |
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Term
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Definition
-opportunistic pathogen activated by immunosuppression |
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Term
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Definition
-hand-foot-mouth disease
-highly contagious spread by oral fecal route
-herpangia (coxsackie virus)
-vesicles on soft palate
-measles (rubeola)
-vaccine
-german measles (rubella)
-vaccine
-fifth disease (parvovirus B19)
-"slapped cheeks"
-respiratory |
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Term
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Definition
-inflammation of the liver
-toxic
-infectious
Types A, B, C, D, E |
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Term
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Definition
-infectious
-picornavirus
-capsid RNA
-oral- fecal route |
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Term
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Definition
-serum
-hepadnavirus
-enveloped DNA
-sexual contact |
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Term
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Definition
-NonA/NonB
-flavivrus
-enveloped RNA
-sexual contact |
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Term
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Definition
-Delta agent
-viroid like
-enveloped circular RNA
-sexual contact |
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Term
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Definition
-enteric
-calicovirus \
-capsid RNA
-oral fecal route |
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Term
serum components and indications |
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Definition
-IgM anti HAV or IgM antiHBc indicate recent or active hepatitis infection
-Anti HAV; AntiHbs; Antidelta indicate a past ifnection with hepatitis |
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Term
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Definition
-enveloped virion
-very stable and resistant to low pH and temperature extremes
-easy to transmit
-envelope consists of Hep B surface antigens |
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Term
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Definition
-virus binds to hepatocyte
-enters cell endocytotically
-carried to nucleus
-uncoats
-replicates
-goes into membrane vacuole and makes nucleid capsid
-excess capsid particles are released when virus exocytoses |
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Term
manifestations of Hep B (3) |
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Definition
-Acute
-Chronic
-Fulminant |
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Term
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Definition
-90% of cases
-cell mediated immune response shuts down virus and yields resolution |
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Term
acute hep B manifestations |
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Definition
-subclinical, mild, or severe symptoms
-severe classified as having jaundice for 4-5 wks
-incubation period of 4-26 wks
-preicteric phase-prodromal symptoms
-icteric phase-jaundice and dark urine results from build up of bilirubin which liver can't break down
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Term
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Definition
-over period of months
-damage to liver causes spike in liver enzymes
-at beginning you see HBsAg so you know virus is replicating
-towards end you will see anti-HBsAg IgG and yhou know virus is resolved
-between those two stages you have the antigen Hep B window where neither are present in serum bc they are binding each other up. |
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Term
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Definition
-9% of cases
-cell mediated immune system doesn't clear virus, but does slow down virus development over span of 6 months +
-half of these cases resolve
-half of these cases are chronic and active and may cause ultimate damage (cirrhosis and carcinoma) |
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Term
chronic Hepatitis B manifestations |
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Definition
-in chronic persistant hepatitis, one is asymptomatic, but still sheds the virus
-in chronic active hepatitis, there can be development of carcinoma, cirrhosis, or liver failure but symptoms prior to this point are more mild than acute |
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Term
chronic hepatitis B serology |
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Definition
-over span of years
-redundancy in liver may cause enzyme levels to fall
-trace the HbsAg & antiHbsAg to detect infectious status
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Term
chronic Hepatitis B vaccine |
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Definition
-inactivated HBsAg from human serum (old)
-recombinant DNA vaccine with HBsAg gene inserted into plasmid (current) |
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Term
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Definition
-picornavirus
-very stable in environment for long periods of time and can be frozen for months |
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Term
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Definition
-liver cell receptors
-classic RNA replication/cytoplasmic replication
-uncoats and replicates in the cytoplasm
-causes cell to lyse |
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Term
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Definition
-usually via oral-fecal route/contaminated food & water
-after ingestion, virus survives low pH of stomach and crosses intestinal wall into blood stream causing transient viremia
-virus gets dumped into liver and the virus causes cell lysis there
-virus is dumped into bile where it still survives and gets dumped back into the intestine
-virus is excreted in fecal matter to start process again |
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Term
clinical syndromes of Hep A |
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Definition
-much shorter incubation period than B
-abrupt onset of symptoms
-children tend to be asymptomatic bc of highly responsive cell mediated immune system]
-virus is small and will stay in circulation even when you see presence of antibodies and virus may deposit elsewhere (cell walls and joints)
-rash and arthritis are rare though
-tx: interupt contamination and give immune serum Ig during incubation phase
-this is 80-90% effective |
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Term
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Definition
-short or long incubation period (avg is about 6-9 wks)
-milder symptoms then Hep A/B
-icteric phase seldome develops
-still see chronic damage though
-10-50% of cases become chronic
-chronic case increases risk of recurrence in liver transplant
-fulminate decreases risk of infectio nof liver transplant
-tx: none; but 10-40% cases respond to interferon and ribivinn tx
-diagnosis: eliminate possibility of other two types |
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Term
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Definition
-defective satellite virus
-can't replicate unless Hepatitis B is suprainfected or Co-infected in same cell
-coinfection increases risk of fulminate hepatitis and slightly increases the risk of cirrhosis and carcinoma
-complicates but does not cause hepatitis by itself
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Term
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Definition
-enterically transmitted (oral-fecal route)
-diagnose by excluding other Hep viruses
-similar to HAV clinically
-short incubation time
-abrupt onset of symptoms
-no chronic form
-no tx or prevention |
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Term
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Definition
-filterable, small agents
-non living
-require host cell's machinery to replicate
-have either RNA or DNA genome, but not both
-are naked capsid type or enveloped
-must encode any required processes not provided by host cell and package specialized proteins and nuclear binding proteins and enzymes |
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Term
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Definition
Naked
DNA/RNA+structural proteins+nuclear binding proteins and enzymes=nucleocapsid
nucleocapsid+envelop (membrane and glycoproteins)=envelope |
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Term
DNA viruses
name and differentiate between ds or ss, and enveloped or naked |
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Definition
-enveloped: Pox, Herpes, hepadna
-Naked: polyoma, papilloma, adeno and parvo (ss) |
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Term
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Definition
-virus has tissue tropism
-attaches to target cell via glycoproteins to bind
-immn syst recognizes these glycoproteins when in circulation after cell lyses
-we use these glycoproteins to create vaccines
-glycoproteins fuse cell membranes to make gigantic cells withe several nuclei |
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Term
RNA viruses (15)
define if ds or ss; and if + or - stranded |
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Definition
ds: reo
ss+ : picorna, corona, noro, toga, flavi, hepatitis, retro
ss - : orthomyxo, paramyxo, rhabdo, filo, borna, bunya, arena,
* negative indicates that it is enveloped |
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Term
places where drugs can interact to stop viral infection |
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Definition
-Antibodies prevent virus from attaching
-drugs could stop viral uncoating
-drugs could stop viral transcription and replication |
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Term
steps in viral replication |
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Definition
1-recognition and attachment
2-penetration
3-uncoating
4-macromolecule synthesis (mRNA & nonstructural proteins; genome replciation; late mRNA & structural proteins; modification of proteins)
5-assembly
6-budding of enveloped virus OR lysis of non enveloped virus
7-release of virus |
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Term
viral protein synthesis and protein replication of ds DNA
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Definition
-DS DNA makes mRNA and proteins via host machinery |
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Term
viral protein synthesis and genome replication of ss DNA |
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Definition
-make complement strand of DNA
-make mRNA
-make proteins |
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Term
viral protein synthesis and genome replication of positive stranded RNA |
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Definition
-positive stranded RNA is like the mRNA that binds to ribosomes and makes one polyprotein that is then cleaved
-the mRNA makes a template of - RNA to create +mRNA progenies |
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Term
viral proteins synthesis and genome replication of - stranded RNA |
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Definition
-RNA is transcribed into mRNA segments and then proteins are made
-RNA is also made into a full length +RNA template to make (-) RNA progenies by RNA polymerase |
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Term
viral protein synthesis and genome replication of ds RNA |
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Definition
-acts like (-) stranded RNA
-transcribed to make mRNA segments by RNA polymerase in capsid |
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Term
viral protein synthesis and genome replication of retrovirus |
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Definition
- (+) stranded RNA are converted to complementary DNA by reverse transcriptase carried in a virion |
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Term
-immediate early and early phase viral proteins are _____1________ for ______2__________ ____________ are produced. an example is ________3_______ carried with virus |
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Definition
1-necessary
2-DNA synthesis
3-RNA dependent RNA polymerase |
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Term
late genes most encode viral ____________ proteins which requires viral DNA synthesis |
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Definition
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Term
papilloma virus characteristics and pathology |
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Definition
-small, nonenveloped icosahedral virus; ds DNA
-encode proteins that promote cell growth
-may oncogenetically transfer a cell that is non permissive
-causes warts and human cervical carcinoma
-infects squamous epithelium & mucosal membrane
-virus accesses the basal cell layer through breaks in skin causing basal cell to grow and cell # increases
-this leads to thickenings and causes the wart/papilloma |
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Term
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Definition
-E1 protein binds to DNA @ origin to promote replication
-E6 and E7 are immortalizing genes
-E6 binds w p53 protein and targets it for degradation
-E7 deactivates p105RB; thus NO BRAKES on cell growth |
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Term
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Definition
-like HPV
-T antigen analogous to E6&E7
-JC virus establishes kidney infection
-BK virus establishes latent kidney infection
-respiratory tract-->viremia-->kidney inf.-->viremia-->
-immunocompetent patients experience latency
-reactivation of BK virus causes hemmorrhagic cystitis in urinary tract
-reactivation of JC virus is in the CNS |
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Term
adenovirus structure characteristics and replication |
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Definition
-capsid made of capsidmeres
-spikes protrude as ligand receptors and are made of glycoproteins
-nonenveloped icosadeltahedron
-potential to become oncogenic
-replication:
-requirements for transcription of early proteins: E1 gene transcription; process of primary transcript; translate immediate early E1A transactivator protein (controll/growth supressor)
-end result is host cell death |
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