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Plasmodium spp ID Methods |
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Definition
thick and thin blood smears, Antigen testing (Rapid EIA) |
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trophozoite shows signet rings in rbcs, rare gametocytes seen in blood film, schizont in liver shows Merozoites, when schizont ruptures Merozoites enter the blood stream, Merozoites become trophozoites in the rbcs, cannot be seen extracellularly |
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Plasmodium spp special characteristics |
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Definition
species have different characteristics, seen in patients who have traveled |
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Definition
thick and thin blood smears, IgM/IgG antibody detection and PCR |
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Definition
ringed trophozoites on thin film, multiple rings and Maltese- cross, can be seen extracellularly, |
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Babesia spp special characteristics |
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Definition
seen in United States (east coast) |
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Toxoplasma gondii ID methods |
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Definition
serologic methods to test for IgG/IgM in Buffy coat, PCR on blood and amniotic fluid, microscopic detection on blood smear or tissue section |
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Toxoplasma gondii diagnosis |
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Definition
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Toxoplasma gondii special characteristics |
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Definition
from cat feces, detected in blood or tissue, can cross placenta |
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Trypanosoma spp ID methods |
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Definition
blood smear (also CSF, chancre, spleen, liver, bone, lymphatic system, heart and brain), ELISA and agglutination methods (immunologic methods) |
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Trypanosoma spp diagnosis |
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Definition
undulating membrane, thin half moons, big, flagella |
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Trypanosoma spp special characteristics |
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Definition
causes African sleeping sickness, speciate by PCR |
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Leishmania spp ID Methods |
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Definition
blood smear, serology and immunology techniques, and PCR |
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Definition
long skinny tapered promastigote in blood |
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Special characteristics Leishmania |
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Definition
macrophage ingests promastigote, amastigote in tissue samples, flagella |
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