Term
|
Definition
|
|
Term
| What reagent does DAT use? |
|
Definition
| Antihuman Globulin Reagent (AHG) |
|
|
Term
|
Definition
| ABY or Complement bound to RBCs |
|
|
Term
| Is DAT testing in vitro or in vivo? |
|
Definition
| In Vivo (inside the body) |
|
|
Term
|
Definition
| Indirect Antiglobulin Test |
|
|
Term
| What reagent does IAT use? |
|
Definition
| Antihuman Globulin Reagent (AHG) |
|
|
Term
|
Definition
| ABY boung to cell surface |
|
|
Term
| Is IAT testing in vivo or in vitro? |
|
Definition
| In Vitro (in the test tube) |
|
|
Term
| Why were both tests developed? |
|
Definition
| To see if cell sensitization has occurred with the IgG ABY or Complement |
|
|
Term
| What class of ABY is called incomplete or is known as sensitizing antibodies? |
|
Definition
|
|
Term
True or False:
Even if we can't see the sensitization, it CAN have clinical affects |
|
Definition
|
|
Term
| Most _____ ABYs are formed only as a result of exposure through either prior transfusion or pregnancy |
|
Definition
|
|
Term
True or False:
Having immunoglobulin or C' coating RBCs is NOT a normal condition |
|
Definition
|
|
Term
What is the following an example of?
"HDN with Anti-D: baby could be very anemic from cells being destroyed but we could not demonstrate it in the lab because of no agglutination" |
|
Definition
|
|
Term
| 5 things which affect sensitization |
|
Definition
AGN/ABY ratio
pH
Ionic Strength
Temperature
Time |
|
|
Term
|
Definition
|
|
Term
| Sensitization is more likely when ____ is at a higher ratio |
|
Definition
|
|
Term
| What should you use to help the ionic strength? |
|
Definition
| Use LISS which has fewer (+) ions, and more readily allows binding |
|
|
Term
| 5 Things which affect Agglutination |
|
Definition
Medium potentiators such as albumin
Enzyme treatments
Centrifugation
Class of Immunoglobulin
Polyethylene Glycol |
|
|
Term
| Is Polyethylene Glycol good for detecting weak antibodies? |
|
Definition
|
|
Term
True or False:
Hemolysis from C' Activation is another sign that AGN and ABY have joined |
|
Definition
|
|
Term
True or False:
C' activation is triggered when an ABY has attached to an AGN |
|
Definition
|
|
Term
True or False:
Only some ABYs activate C' |
|
Definition
|
|
Term
True or False:
Sometimes C' activation does not proceed through the whole sequence to cell destruction but stops leaving pieces attached to the cell |
|
Definition
|
|
Term
| Which pieces might be left attached to the cell if C' activation does not proceed all the way to destruction of the cell? |
|
Definition
|
|
Term
| Is it important to know if C3 is attached to cell? |
|
Definition
| Yes, it means that AGN/ABY reaction occurred even if not enough to cause visible hemolysis |
|
|
Term
|
Definition
Monospecific
C'
Polyspecific
Monoclonal |
|
|
Term
|
Definition
| Use when don't want to detect IgM ABYs which are usually clinically insignificant |
|
|
Term
|
Definition
| Reacts only with bound C' |
|
|
Term
|
Definition
| Will react with RBCs coated with IgG or C' |
|
|
Term
|
Definition
| Use for DAT when we want to know if anything is coating the cell |
|
|
Term
|
Definition
| produced by hybridoma in vitro |
|
|
Term
|
Definition
HDN
Transfusion reaction
AIHA
Drug Induced Reactions |
|
|
Term
|
Definition
| See if mother's ABY has crosses placenta and attached to baby's RBCs |
|
|
Term
|
Definition
if DAT (-) before transfusion and DAT (+) after, the patient had ABY which attached to donor RBCs
AKA: wrong blood given |
|
|
Term
|
Definition
| Detect if autoABY is coating cells making them prone to destruction by the spleen or being made into spherocytes |
|
|
Term
|
Definition
Wash patient's RBCs with saline
Add AHG
Centrifuge
Add Check Cells to any = reactions |
|
|
Term
| Sample - prefer _____ b/c the absence of Ca prevents in vitro sensitization |
|
Definition
|
|
Term
|
Definition
| Various procedures to remove the protein coating the cells |
|
|
Term
| Bound ABY is removed by ____, change in ____, or ______ |
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
| Eluate + known RBC AGNs to identify what was on the cells |
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