Term
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Definition
-Results in chronic myelogenous (granulocytic) leukemia
-From a reciprocal translocation between 22 and 9 (not Robertsonian) |
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Term
| Denaturing Gel Electrophoresis? |
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Definition
-Uses a gradient in the gel of a denaturing chemical that becomes increasingly more intense
-differences in fragments of the same length often cause them to partially melt at different positions in the gradient and therefore "stop" at different positions in the gel |
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Term
| DNA sequence analysis for disease detection? |
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Definition
-Uses ddNTPs to stop synthesis at random lengths
-Great for point mutations and highly specific
-However, doesn't usually include promotor regions, isn't very good at detecting deletions or insertions of exons, and you need to know what your looking for |
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Term
| What are the three big direct methods of genetic testing? |
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Definition
-PCR analysis
-Southern blot (and all the other blots)
-Sequencing |
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Term
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Definition
-Log of Odds
-Broadly, it is used to asses the degree of genetic linkage for loci (tendency to be inherited together)
-A score of 3 or more is needed for there to be linkage
-The higher the score, the more closely linked the loci (closer they are on a chromosome)
-Can be used to map genes out, but requires lots of offspring otherwise genes might be inherited together by chance alone |
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