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*Binds DNA called the silencer to regulate transcription *bound by silencer to regulate transcription |
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*Initiates transcription by attracting RNAP to the +1 location *initiates transcription by binding the CAT and TATA boxes |
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Small protein added to the end of a protein to target it for degradation |
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*Part of both replication and DNA packaging to help keep DNA from being too wound up *enzyme in DNA synth, no new strands of nucleic |
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Can change how many proteins are encoded by a gene |
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Small RNA that targets specific mRNAs for degradation |
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Something that can interact with a protein to change its activity |
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*Helps to stop transcription *when RNA Pol falls off |
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Helps to stop translation |
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Degrades specifically tagged proteins |
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Makes RNA that is complementary to DNA (for replication) |
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Binds DNA called the enhancer to regulate transcription |
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*transcription binding protein *initiates transcription by binding TATA boxes in euks |
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specifically tags proteins with other proteins for degredation |
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*process of remiving RNA from mRNA in euks |
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does NOT recognize stop codon to allow translation termination |
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does recognize stop codon to allow translation termination |
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Processes that ensure there are few mistakes when DNA Is replicated |
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selection of the correct base by hydrogen bonding between the DNA and new base |
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has free phosphate ends (no ester link) |
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*separated DNA inside bubble |
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*key enzyme for synthesizing DNA *BIOCHEM FUNC: 5'- 3' DNA polymerase 5' - 3' exonuclease 3' - 5' exonuclease |
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Enzymes in DNA synthesis w/ no capacity to create new strands of DNA |
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Primase Topoisomerase Helicase |
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Enzymes in DNA synthesis that can create new strands of nucleic acid |
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DNA polymerase 1 DNA polymerase 3 Primase |
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*a specific DNA sequence that is bound by proteins |
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involves protein for direct function |
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compound involved in tRNA formation |
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compound involved in DNA replication |
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compound involved in miRNA formation |
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*allows packaging of DNA *allows the cell to control transcription of DNA |
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*required to remove incorrect bases during replication (3' - 5' exonuclease) *required to remove RNAs during replication (5' - 3' exonuclease) |
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Prevents strong expression of Lac Operon |
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*PRESENT: glucose ABSENT: CAP-cAMP
PRESENT:glucose PRESENT:glucose catabolite |
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Promotes strong expression of Lac Operon |
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PRESENT:CAP-cAMP ABSENT:glucose |
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RNA polymerase bound when transcription bubble opens |
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*same box w/ promoter *one before |
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Stop codon RNA seq in same read frame as AUG |
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*promoter box *protein ORF 2 *after ORF 2 |
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Ribosome binds @ translation start |
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elements that regulate RNA of mRNA accumulation (euk terminates E) |
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Sigma factor bind to allow RNAP to start transcription bubble and allow 1st base to be incorpoarted into RNA |
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Ribosome binds @ translation end |
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Stop codon RNA seq in diff read frame as AUG |
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Minimal size for functional RNA (B-D) protein 100 AA |
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*euk trancripts have a cap structure that is 3' - G - 5' - PPP - 5'N *introns in all euk transcripts marked by sequences at 5' and 3' ends of introns |
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tRNAs exit ribo EPA Translation |
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tRNAs enter ribo EPA Translation |
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tRNAs ANY exit ribo EPA Translation |
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@ start translation, where tRNA w/ AA chain EPA Translation |
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@ end translation, where tRNA w/ AA chain EPA Translation |
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during translation, tRNAs w/ AA EPA Translation |
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during translation, tRNAs w/o AA EPA Translation |
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Scans region A to find start codon EPA Translation |
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Components bound to allow initiation EPA Translation |
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initiation factor (bound prior) 16S RNA 30S Ribosome |
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Information contained ensure tRNA has correct AA attatched EPA Translation |
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Information contained ensure tRNA adds correct AA to growing chain EPA Translation |
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part of translation uses only RNA/ protein in some form EPA Translation |
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*recognition of shine delgarno *recruitment of next tRNA *termination *growth of polpep chain |
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provides E for translation EPA Translation |
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a specific DNA seq bound by protein |
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How DNA Pol 1 minimizes errors in DNA replication |
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*selection of correct base by H bond betweenn DNA and new base *3'-5' exonuclease mediated proffreading activvity |
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Parts of diagram w/ no RNA |
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*outside buble DNA strand |
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*DNA synt only goes 5'-3' *DNA synth bidirectional |
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*a specific RNA proteins bound by protein |
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Involves only protein for activity |
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*ability to bend w/o crack |
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*ability to bend w/o crack *largest pocket for transcription factors to interact w/ new bases |
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*diester *bonds critical hold DNA together *deoxy ribose *key info in bond histones |
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*Hbond *N *bonds critical to hold DNA together *transcript fact trying to access DNA pol *aromatic rings |
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*repressor i, i- = inducible transcrip i-, i-= always transcrip is = never transcrip
is > i+ > i- |
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*promoter *no p = no o * p- = never transcrip
p+ > p- |
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*operator * oc = always transcrip oc, oc = >is, always transcrip o= bind repressor (no lact)
o+ > oc |
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