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Any technique that uses living organisms, or parts of organisms, to make or modify products, improve plants or animals, or to develop microorganisms for specific uses. |
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1. cheese making 2. wine making |
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1. genetic engineering 2. clonning 3.cell culturing |
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yogurt is a food produced by the fermentation of milk by bacteria |
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bacteria involved in yogurt fermintation |
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1. streptococcus thermophilus 2. lactobacillus bulgaricus |
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lactic acid being formed by the bacteria |
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what gives yogurt the semi solid apperance |
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increase in lactic acid causes the casein micelles to unravel and form a mesh like structure |
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what are the four components of milk |
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1. water 2. fat 3. casein micelles 4. lactose |
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what does streptococcus and thermophilus mean |
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- a chain of spherical bacteria - bacteria can live in high tempritures |
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what does lactobacillus and bulgaricus mean |
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-a rod shaped bacteria that can convert lactose in milk to lactic acid - explains where the bacteria was first seen (bulgaria) |
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what does acetaldehyde do to yogurt |
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it gives it its pungent smell and taste. |
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what is the word and chemical equasion for fermintation of lactose |
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lactose - lactic acid + 2ATP
C6 H12 O6 - 2C3 H6 O3 |
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what 4 names refer to the shape of bacteria |
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1. cocci 2. bacilli 3. spirilli 4. Vibrios |
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what names sugest cluster and chains of bacteria |
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Definition
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what happens to bacteria when conditions are favourable |
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Definition
reproduce by binary fusion to produce genetically identical daughter cells |
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what happens to bacteria when conditions are not favorable |
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Definition
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what does phytosynthetic mean |
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Definition
capeable of making their own food |
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what does heterophilic mean |
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not capable of making their own food |
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how do heterotrophic bacteria eat |
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Definition
secrete enzimes onto a food source, digest the food outside the cell and then obsorb the souble food into the cell by diffusion |
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what are bacteria called when they do and do not need oxygen |
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Definition
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what is the range of ph levels in which acidophillic, neutrophilic and alkaliphilic bacteria live in |
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enzimes are biological catalysts that are made inside cells but can function internally and externally |
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are enzime activities effected by tempritures |
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yes. when the temp is above 50 deg c when the shape of the active site changes perminently |
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what is enzime activity effected by |
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chemical inhibitors which compete with the subtrate molecule for the enzimes active site. inhibitrs either temporairily or perminitly block the active site. |
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an important molecule of hereditry in all living things. |
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DNA is a double sided molecule made of two chains of neucleotides |
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what else can DNA be described as |
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lader- like molecule where the sides are sugar- phosphate backbones and runs are complementery pairs |
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what are the four bases that pair up |
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Thymine- adenine guanine- cytosine |
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it carries hireditory information in the form of genes |
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a gene is a small section of the DNA molecule which contains a coded message |
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how is the coded message stored |
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as a sequance of bases on one side of the DNA molecule |
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what is a chromosone made up of |
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DNA and contains many genes |
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what is one side of the DNA a coded message for |
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what is a common organism used in genetic engineering |
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Definition
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what are plasmids used for |
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transfer a selected gene into a bacterium |
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what enzime is used to cut the plasmid |
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Definition
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what enzime is used to glue the plasmid back together |
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what is done to the gene for making human insulin |
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found and isolated from the human genome |
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how is the gene transfered to a suitable bacterium |
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using restriction and ligase enzimes the gene can be spliced into a plasmid and transfered |
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why is bacteria a perfect host cell for the human gene |
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Definition
a clone of the identical cells can be producing during binary fussion |
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why is agrobacterium tumefaciens used by genetic engineeres to transfer selected genes into plant cells |
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Definition
its a natural enemy and can be used as a vehicle to transfer genes into food producing plants |
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how is the gene transfered to the plant |
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Definition
spliced into a plasmid and transfered to the agrobacterium tumefaciens |
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what was used in early GE |
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Definition
antibiotic reistance genes called marker genes, where also inserted into the plasmid. some scientists beleive that this is what accelerated the evolution of antibiotic resistance in bacteria |
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how are the agrobacterium tumefaciens transfered into the plant |
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Definition
small peices of plant chosen to be GE are placed in a culture medium and covered with the GE agrobacterium tumefaciens |
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what causes the plant tissue to begin to grow roots and a stem |
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further culturing with growth factors |
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whay are the plants then grown in soil in a glasshouse |
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Definition
to test for characteristics of the gene and to test for problems the GE plant may have on humans, the enviroment and other species. |
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what is GE on human cells called |
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how are diseases such as cystic fibrosis being treated |
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what is the problem with cistic fibrosis patients |
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Definition
too much salt inside their cells which results in an osmotic inbalance and water entering the cells |
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what is the build up of salt due to |
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Definition
a faulty cistic fibrosis transfer gene (CFTR) |
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what is the vector for transfering the healthy cistic fibrosis transfer gene into the lung cells of a CF patient |
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Definition
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what does the CFTR gene do once inside the cell |
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makes a protein that allows the cell membrane to allow salt to leave the cells to restore the correct water levels inside the lung cell |
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what is an alternative method to transfer the genes inside the lung cell |
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fat droplets into which the genes can be hidden. lung cells will accept the liposomes as food and constantly take in the hidden genes |
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what are the 3 benefits of GE |
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Definition
1. cheaper medicine production 2. more nutritionous foods 3. crops better suited to saline soils and drought conditions |
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what are the concerns for genetic engineering |
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1. safety 2. jumping genes may cause an evolution of super weeds 3. acceleration of antibiotic resistant bacteria |
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what are 3 ethnical concerns |
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1. companies like monsanto have a conflict of interest with making pesticides and herbisides as well as GE crops 2. genes can be patented 3. threat to third world countries |
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the study of living organisms in relation to their enviroment |
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the biotic and abiotic features of an enviroment |
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all the populations of animals and plants (biotic factors) occupying a location |
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non living factors in an ecosystem |
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living factors in an ecosystem |
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capable of making their own food (producers) |
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not capable of making their own food (consumers) |
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the study of the relationships between organisms that live in the oceans of the world |
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what are plankton known as |
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what is the most numerous of phytoplanktons |
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what is the remains of dead organisms in the ocean called |
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what are food chains used for |
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to show the feeding relationships and flow of energy in an ecosystem |
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what is at the start of a food chain |
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what does a biomass pyramis show |
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the dynamics of energy in an echosystem |
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what does a food web show |
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all the feeding relationships and flow of energy in an echosystem |
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what do addaption of animals enable them to so |
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survive in their enviroment |
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what do adaptions help an organism to fufill |
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Definition
1. food/ water 2. protection 3. reproduction |
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what are structual adaptions related to |
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physical features of an organism |
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what are behavioural adaptions related to |
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Definition
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what are phsycological adaptions related to |
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the internal functioning of an organism |
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what is the structial adaptation of fins on fish used for |
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the controlled movement in the ocean |
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1. spiny dorsal fin 2. soft dorsal fin 3. caudal fin (tail) 4.anal fin 5. pelvic fin 6. pectoral fin |
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all organisms need to exchange gass with their enviroment by what |
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what are the 3 characteristics of gass echange surface to increase the rate of difusion |
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1. large surface area 2. thin 3. moist |
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what needs to difuse in and out of fishs blood |
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extract oxygen from the water |
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how is the rate of diffusion increased |
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counter- current system where the water and blood flow in oppisate directions |
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what are the 5 characteristics of sewage |
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99% water disolved organic matter high nutrient levels ten high levels of heavy metals chemical toxins |
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how do toxic algal blooms begin |
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discharge of sewage with high neutrient levels which accelerate the growth of blue green algae |
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what does an algal bloom result in |
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depletion of oxygen death of organisms due to the build up of toxins in the water |
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what is the depletion of oxygen in water called |
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what does sewage with high organic matter do |
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provide food for aerobic bacteria as they consume the organic matter they demand oxygen |
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