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Biosystems II
Gene Tranfer
14
Biology
Graduate
03/06/2012

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Term
transformation
Definition
-'competent' bacteria
-take up floating DNA
Term
transduction
Definition
-infection by bacteriophage
-virus may incorporate host DNA into bacteriophage capsid via homologous recombination
-ex.vibrio cholerae-code for exotoxin
Term
conjugation
Definition
-bacterial mating
-dependent on plasmids containing TRA genes
-encode for sex pilus assembly (F-pilus)
-DNA is transferred via sex pilus
-can transfer antibiotic resistance genes
Term
transposition
Definition
-movement of DNA from DNA molecule to DNA molecule
-plasmid to genome, virus to plasmid, within plasmid
-movement due to transposable elements (jumping genes)
-transposase is enzyme responsible for excision and insertion
Term
salmonella
Definition
-gram negative
-serotypes defined by O and H (flagellar) antigens
-most common fermenter in animal GI tract
-common cause of diarrhea
-fecal oral transmission
Term
salmonella infection
Definition
-produce NO exotoxins
-inflammatory response triggers prostaglandins triggers cAMP triggers fluid secretion
Term
C. diphtheriae
Definition
-gram positive
-B binds
-internalization
-releases A
-inactivates EF-2
-inhibits protein synthesis
-cell death
-damages?
Term
V. cholerae
Definition
-curved gram negative
-B binds
-internalization of A
-activates AC
-increases cAMP
-releases ions/water
Term
B. anthracis
Definition
-gram positive
-anthrax toxin (spores)
-edema-AC activity, increases cAMP
-lethal-protease activity, dec. MAPKK, apoptosis
Term
C. tetani
Definition
-gram positive
-tetanospasmin toxin inhibits inhibtor transmitter
-spastic paralysis
Term
C. botulinum
Definition
-gram positive
-toxin blocks release of ACh from vesicles
-flaccid paralysis
Term
Enteropathogenic E. coli (EPEC)
Definition
-do not produce toxins
-virulence factor is Intimin (adhesion)
-Tir codes for receptor for Intimin
-loss of microvilli in small intestine (loss of absorption)
-A/E lesions?
Term
bacterial entry zipper mechanism
Definition
-bind receptors
-engulf by actin
Term
trigger mechanism
Definition
-type 3 secretion
-protein is sent to activate GTPase/actin polymerization
-engulfed
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