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Biology210A - Exam 2
Study Guide for Exam 2
71
Biology
Undergraduate 2
09/18/2013

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Term
Sidedness
Definition
Location relative to cell
Term
ER Lumen
Definition
Where synthesis of ribosomes occurs
Term
Membrane Transport
Definition
Transport of substances through a selectively permeable membrane
Term
What is the hardest portion of the membrane to permeate?
Definition
The hydrophobic region
Term
The phospholipid bilayer is permeable to
Definition

Hydrophobic, non-polar, and small substances

(i.e. O2, CO2)

Term
The phospholipid bilayer is NOT permeable to:
Definition

Large, hydrophilic, polar, or charged substances 

(i.e. Glucose, H2O, Na+, Cl-)

Term
Diffusion
Definition
Movement of a substance from a high concentration to a low concentration until equilibrium is reached
Term
Active Transport
Definition
Transport of substances from a low concentration to a high concentration until equilibrium is reached
Term
Membrane Potential
Definition
Voltage difference across a membrane based on establishment of electrochemical gradient
Term
Cotransporter
Definition
Indirect energy used to Hydrogen ions can diffuse later with sucrose
Term
Bulk Transport
Definition
Transport pathway for large molecules or large quantities of small molecules (requires energy; therefore a form of active transport)
Term
Endocytosis
Definition
Substances go into cell
Term
Phagocytosis
Definition
Cell engulfs substances, a form of endocytosis
Term
Pinocytosis
Definition
Cell "drinks" substances, a non-specific form of endocytosis
Term
Receptor-mediated endocytosis
Definition
Binding of a specific molecule to membrane receptor triggers endocytosis, involves protein-coated vesicles
Term
Physiological Factors Affecting Enzyme
Definition
  1. pH
  2. Temperature
  3. Salinity

There exist optimum levels for each.

Term
Two types of Enzyme Inhibitors
Definition
Competitive Inhibitors and Non-competitive Inhibitors
Term
Competitive Inhibitors
Definition
Has a lot of similarities to substrate and binds to active site instaed of substrate, preventing substrate from binding.
Term
Physiological Factors Affecting Enzyme Function
Definition
  1. pH
  2. Temperature
  3. Salt

There exists an optimum level for each.

Term
Other Factors
Definition

Cofactors:

  • Inorganic: Mg2+ (metal ions)
  • Organic: Coenzymes (NAD+, FADH2)
Term
Types of Enzymatic Inhibitors
Definition
  • Competitive Inhibitor
  • Non-competitive Inhibitor
Term
Competitive Inhibitors
Definition
Have a lot of similarities to subdstrate and binds to active site instead of substrate preventing substrate from binding. This binding is reversible, meaning that an increased substrate concentration can "kick out" inhibitor from active site.
Term
Non-competitive Inhibitors
Definition
Binds to a different site of the enzyme, or allosteric (other) site. This contorts the active stie of the enzuyme preventing binding of the substrate to active site. This process is irreversible, meaning that an increase in substrate does not reverse inhibition.
Term
Why would it be a good thing to inhibit an enzyme?
Definition
To stop an unnecessary process
Term
Allosteric Regulation
Definition
Binding to enzyme at allosteric site to activate or inhibit
Term
Activator
Definition

Binds to allosteric site to stabilize active enzyme form

(active site open)

Term
Inhibitor
Definition
Stabilizes inactive form (active site closed)
Term
Cooperativity
Definition
Type of allosteric regulation in which substrate binding to one subunit's active site stabilizes active sites of other subunits
Term
Feedback Inhibition
Definition
The end product of a multi-step reaction serves as an allosteric inhibitor to one of the first enzymes in the process (when large amounts of end product accumulate)
Term
In cellular respiration, the _______________ are the __________________ of photosynthesis.
Definition
Reactants, products
Term
Oxidation
Definition
Loss of Electrons (LEO)
Term
Reduction
Definition
Gaining of electrions (GER)
Term
Summary Reaction of Cellular Respiration
Definition

C6H12O6 (Glucose) + 6O2 → 6CO2 + 6H2O + Energy (ATP)

 

Term
In cellular respiration, _________________ is reduced and ___________________ is oxidized.
Definition
Oxygen, Glucose
Term
Redox reactions are carried out by _________________ and require the help of __________________
Definition
Dehydrogenases, electron carriers
Term
The Three Stages of Cellular Respiration
Definition
  1. Glycolysis (in cytosol)
  2. Pyruvate conversted to Acetyl CoA/Citric Acid Cycle (mitochondrial matrix)
  3. Oxidative Phosphorylation (inner membrane)
Term
How is ATP synthesized?
Definition
  1. Oxidative Phosphorylation (where most of the ATP is made)
  2. Substrate-level Phosphorylation (where a relatively small amount of ATP is made), enzyme plucks one phosphate off a substrate and adds it to ADP to make ATP
Term
Glycolysis
Definition

1 Glucose → 2 pyruvate + 2NADH + 2ATP

 

Phosphofructokinase is allosterically regulated.

Term
AMP
Definition

Adenosine Monophosphate

 

Stimulates phosphofructokinase by changing the shape of the active site to its correct shape and process can continue; acts as an activator

Term
An abundance of citrate can act as a(n) ____________.
Definition
inhibitor
Term
What happens during photosythesis (generally)?
Definition
Solar energy (energy from the sun) is converted into chemical energy in a cell (i.e. sugars).
Term
Autotroph
Definition
  • Organism capable of creating its own food source
  • Also known as a producer
  • Convert inorganic energy into usable chemical energy
  • i.e. Photoautotroph
Term
Photoautotroph
Definition
An autotroph that carries out photosynthesis, gets energy from the sun (i.e. algae being used for biofuels)
Term
Summary Reaction of Photosynthesis
Definition

It's essentially the opposite of celluar respiration:

 

6CO2 + 6H2O → C6H12O6 + 6O2

Term
Photosynthesis is divided into two types of reactions:
Definition
  1. Light Reactions
  2. Calvin Cycle (NOT dark reactions)
Term
Light Reactions take place on the ________________.
Definition
thylakoid membrane (primarily)
Term
In light reactions, ATP is synthesized through ____________________.
Definition
Photophosphorylation
Term

In the photosynthesis summary reaction, ___________ is reduced and ___________ is oxidized.

Definition
CO(Carbon Dioxide), H2O (Water)
Term
During light reactions, _______ is produced and __________ carries electrons.
Definition
O2, NADPH
Term
The Calvin Cycle takes place in the ______________.
Definition
Stroma
Term

In the Calvin Cycle, ATP and NADPH are used to _____________________.

Definition
reduce COto sugar
Term
Photons
Definition
Particles of Light
Term

The shorter the wavelength, the _______________ the energy.

 

The longer the wavelength, the ________________ the energy. 

 

Definition

higher, lower; it's an inverse relationship

 

[image]

Term

Any light absorbed is ___________; any light not absorbed is _____________ or ______________.

 

 

Definition

used; reflected, transmitted

 

 

Term
Absorption Spectrum
Definition

Graph plotting light's absorption (by different pigments in chloroplasts) vs. wavelength of light

 

[image]

Term
Action Spectrum
Definition

Graph plotting rate of Photosynthesis vs. Wavelength of Light

 

[image]

Term
Amount of ______ _________________ is used to measure the rate of photosynthesis.
Definition
O2 released
Term
Theodor Englemann
Definition
  • Laid filamentous algae (photosynthetic) acros microscope slide
  • Shone light refracted through prism onto slide
  • Added aerobic (O2-requiring) bacteria
Term
Where did the bacteria congregate in Englemann's experiment?
Definition

Where O2 was produced (since they were O2-requiring bacterium), virtually everywhere except where green light was. This is because green light is reflected (since ya know, plants are green and stuff). All of the OTHER colors were used.

 

This is a physical example of an action spectrum. 

 

[image]

Term
Pigments in Light Absorption
Definition
  • Chlorophyll a
  • Chlorophyll b
  • Carotenoids
Term
Of the three pigmens involved in light absorption, which are accessory pigments?
Definition
Chlorophyll b and carotenoids
Term
Chlorophyll is part of a ____________________ embedded in the ____________________ of the cell.
Definition
photosystem (PS), thylakoid membrane
Term
Photosystem ___ comes first; Photosystem ___ comes second.
Definition
II, I
Term
ATP from chloroplasts are synthesized in the ________.
Definition
Stroma
Term
The light reactions are divided into:
Definition
Noncyclic (Linear) and Cyclic reactions.
Term
_____________________ is the final electron acceptor of the Calvin Cycle.
Definition
NADP+
Term
In order to go into the Calvin Cycle, ____________ and ____________ are needed.
Definition
ATP, NADPH
Term
In PSII, light is absorbed at a wavelength of
Definition
680 nm
Term
In PSI, light is absorbed at a wavelength of _______.
Definition
700 nm
Term
Cyclic Reactions involve:
Definition
PSI only, ATP only
Term
Noncyclic reactions involve:
Definition
  • ATP AND NADPH
  • PSI AND PSII
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