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Biological catalysts. Either partially or completely proteins with highly specific functions. |
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A compound which speeds up a chemical reaction without being used up or altered in the reaction by lowering the activation energy |
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Material with which the catalyst reacts |
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Part of enzyme that will bind with a substrate |
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formed when an enzyme and substrate bind at the active site. Place where catalysis occurs |
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Factors Affecting Enzyme Activity |
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pH, temperature: change 3D shape specific chemicals: bind and alter shape |
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Chemicals that MUST bind with an enzyme in order for the ENZYME to be active |
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nonprotein subunits that usually bind to the active site on the enzyme and are essential for the enzyme to work. can include metal ions or organic compounds |
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organic cofactor (subunit necessary for an enzyme to work) |
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chemicals that shut off enzyme activity (2 kinds) |
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Competitive Inhibition (what it does, how to fix it) |
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When a molecule that is structurally similar to the substrate competes for a position at the active site on the enzyme. Can be reversed if the concentration of the substrate is raised |
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Noncompetitive Inhibition (what it does- 2 ways, how to fix it) |
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An inhibitor binds to a part of the enzyme that is NOT the active site, causing a change in the nature of the enzyme so that the enzyme's catalytic properties are lost. 1)noncompetitive inhibitor physically blocks active site 2) causes a conformational change in the protein which inactivates the active site Can NOT be reversed by changing concentration |
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Measuring Enzyme Activity (2 ways) |
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1) determine rate of disappearance of the substrate 2) determine the rate of appearance of the product |
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an ANAEROBIC process for transferring the energy in glucose bonds to bonds in ATP (adenosine triphosphate) Can produce a maximum of 2 ATP molecules |
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an AEROBIC process for transferring the energy in glucose bonds to bonds in ATP (adenosine triphosphate) Can produce a maximum of 38 ATP molecules |
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