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Where would you find a lot of Golgi? |
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Where would you find a lot of mitochondria? |
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Where would you find a lot of Smooth ER? |
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-lack nucleus -cytoplasm surrounded by plasma membrane and outer cell wall -may have flagella and pili -bacteria have cell walls |
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-complex internal structures (organelles) -Membranous organelles: nucleus, ER, Golgi apparatus, mitochondria, lysosomes and peroxisomes Non membraneous organelles: ribosomes, microtubules, centrioles, flagella, and cytoskeleton |
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-plant cells: plasma membrane, cell wall, central vacuole and chloroplast |
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Four functional groups of organelles |
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Manufacture Breakdown Energy processing Support, movement, communication |
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-genetic control center -surrounded by double membrane -nuclear pores allow passage of material to nucleus -DNA present -organelles within responsible for RNA synthesis -nuclear laminant: holds nuclear membrane during division |
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contains ribosome synthesis makes protein |
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synthesizes lipids processes materials acts as a storage and detoxifies substances -EX liver cells |
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Vescicles: Synthesis and packing of secretory protien |
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Go to Golgi apparatus carry cargo in them cells tell them where to go signals are sent to them |
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-UPS sorts, warehouses, modifies, packs, and reships substances. -Recieves products from the ER (vescicles) -modifies products that are sent to it in vesicles and sends them out -Puts Zip codes on products so they know where to go in the cell |
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X chromosome factor goes in vesicles and never goes to Golgi so that factor doesn't go into your blood |
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-contain corrosive enzymes *degrade - if defective they cause fatal diseases Tay Sacks disease -help recycle material Ex liver cells -work in your white blood cells -neurological maintenance |
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-ribosomal risessive they go backwards in development mentally enzyme does not work in lysosome nervous system cant be maintained |
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cell maintenance plants hold organic substances storage bin only human cells with vacuoles are fat cells |
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powerhouse of the cell double membrane site of cellular respiration inner membrane outer membrane matrix and cristae |
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Mitochondria ETC located on cell wall |
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Cristae: Matrix: Intermembrane |
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-Inner membrane, folding within mitochondria - between cristae -between inner and outer membrane |
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detoxify inside white blood cells fry germs take water turn it into hydrogen peroxide take hydrogen and turn it into water by removing H |
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Detoxification: Degradation: |
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-Neutralizes something in reactions -Chop something up into pieces |
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internal cell skelet 3 things -microfilaments -intermediates -microtubioles |
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3 components of the cytoskeleton and function |
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Microfilaments: Made of actin, makes them able to bear tension Ex muscles made of actin -function: tension of cell muscle contraction Intermediate: made of many types of proteins -function: skeleton of the cell, more permanent fictures of cytoskeleton Microtubule: Made of tubular, changes shapes can get longer or shorter by adding and removing subunits -functions: things that can grow and get smaller Ex-cilia, flagellum, microtubules move chromosomes apart |
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Cilia and Flagells Structure consists of of 9 microtubule doubles around a central pair 9+2 LOOK AT SLIDES FOR MOVEMENT |
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passes through the membrane easily without transporter |
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Large and Charged molecules: |
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Need transport channels to pass through membranes |
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water channel that lets water through |
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2 cells opposite dirrections |
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-embedded proteins (intergral proteins) -cholesterol -peripheral proteins |
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Chloridic channels dont work |
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integral: transmembrane go all the way through membrane. Amphipathic-charged and non charged regions Peripheral proteins: membrane surface |
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The proxy that powers oxidative phsophoralation |
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Kinetic energy when protons(Hydrogen) diffuse down the gradient (Facilitative Diffusion) |
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Happy flower Wilted flower What happened to the water in the wilted flower? |
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Celery was crisp then it wilted. Why? Celery was wilted then became crispy again |
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Hypertonic solution Hypotonic solution |
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For each glucose that enters glycolysis how many NADH are produced by Kreb cycle? |
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Amino acid valine is transported into the cell by a transporter. It does not recognize any other molecule. |
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Chemiosmotic generation of ATP in the ETC is driven by? |
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Hydrogen gradient Difference in concentration in both sides of the cristae |
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X+ plus We- = Xe- plus W+ Which is oxidized? Which is reduced? |
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none are oxidized X is reduced because it had an electron then lost the electron to W |
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How many Co2 foe each pyruvate? |
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Substrate phosphoralation occurs where? |
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-Krebbs Cycle -Glycolosis -Cytoplasm -Matrix |
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The enzyme (A) 1,4 catalyses (B) 1,3 If (B) 1,3 stops the reaction what is this called? |
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When ATP is made by ATP synthase |
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H+ moves from inter membrane to the matrix Inter membrane= high H+ concentration matrix- low H+ concentration |
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oxidative phosphoralation Chemiosmosis |
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In the ETC who is the final electron acceptor |
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A balloon filled with a water concentration of 55% is put in a beaker of a solution that has a salt concentration of 70%. What happens to the balloon? |
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The balloon shrinks and the water from the balloon goes into the solution. Going from High concentration of water to low. |
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What does phosphorolation do? |
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Increases chemical reactivity increases potential energy through high energy phosphate bond PO4 |
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L:Reactant/Substrate ->Enzyme M: Intermediate N:Product |
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NADH FADH carriers from once cycle to the next |
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If N binds to Enzyme 1 at its active site what is ocuring? |
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If N stops Enzyme 1 What is occurring? |
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Negative feedback feedback inhibition |
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What do glychoproteins do? |
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Cell to cell recognition ID tag of cells Peripheral protien |
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What do transmembrane proteins do? |
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Protein channels intergal proteins Amphypathic |
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What does cholesterol do? |
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Protects against temperature change. |
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LDL is bad. Faulty receptors are supposed to pull LDL out of the blood into the live HDL is good |
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A->B->C DeltaG +6.1 What type of reaction is this? |
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Needs energy (Endergonic/anabolic reaction Matching reaction to get energy: R->S->T DeltaG -7.4 must be greater/equal to what is needed |
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DeltaG is -70KCal add enzymes and heat What is the DeltaG now? |
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Liver cells would contain a lot of? |
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Smooth ER Lysosomes Peroxisomes |
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ATP->ADP+Pi+energy What type of reaction is this? Why? |
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Tight junction anchoring junction gap junction |
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Joined tightly Dont allow materials to pass from cell to cell Ex-Stomach cells have tight junctions |
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Cells are temp/perm joined together located in high friction areas bottom of your feet |
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communicating opposite to tight cell joined together allow materials to flow from cell to cell |
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Diffusion/Passive Diffusion: |
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A substance moving from HIGH TO LOW Does not need help not charged substance can pass through membrane |
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HIGH TO LOW with the help of a protein -EX intergral protein |
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When a substance goes from a LOW TO HIGH (against the gradient) with the help of ATP (energy) endergonic/anabolic |
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Sodium Pottassium Pump NA K uses what? |
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Antiport and active transport |
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things are leaving the cell |
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things enter the cell there are three types |
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regulated receptors on the outside of the cell determine what comes into the cell. |
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High concentration of water and low solution concentration. |
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Low water concentration High solute concentration |
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needs energy +DeltaG absorbs energy also anabolic build up Reactants have low energy Products have high energy |
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-DeltaG gives off energy catabolic break down Reactants have high energy Product has low energy |
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energy cant be created or destroyed Every energy transformation increases entropy everything goes through increase chaos |
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Factors that affect enzymes |
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pH temperature salt co-factors chemicals |
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block active site substrate can't enter |
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Non competitive inhibitor |
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They bind to a secondary site change the shape of the active site of an enzyme substate cant bond with enzyme |
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Feedback inhibition pathway is turned off -excess of something so pathway is stopped product binds with active site and turns it off |
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Poisons that affect the ETC |
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Brotenone DNP Caynide/CO Oligomycin |
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Why do we breath in oxygen? |
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Definition
to catch the hydrogen at the end of ETC to control hydrogen gradient |
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The oxygen (O) we breath bonds to |
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Cytochrome A3 and Hydrogen from the ATP synthase |
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Poison that blocks the 1st protein channel in ETC |
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eats mitochondria (cristae/Innermembrane) creating holles These wholes allow hydrogen to flow freely through the membrane causing the hydrogen gradient to be equalized. Causes an increase in temperature turn RED |
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Cyanide/CO Carbon monoxide |
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Poison binds to the hydrogen and Cytochrome A3 before O (oxygen) can |
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blocks ATP synthase (last protein that spins) |
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Leo-Loses electrons oxidized Girr-Gains hydrogen or electrons reduced |
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gains electrons/hydrogen has potential energy |
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Loses electrons or hydrogen |
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C6H12O6 + 6O2 --> 6CO2 + 6H2O + ENERGY which part of the equation is being oxidized? Why? Which part of the equation is being reduced? why? |
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Carbon is being Oxidized because it lost Hydrogen Oxygen is being Reduced because it gained Hydrogen |
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Intermediaries energy carriers transport hydrogen to ETC |
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use hydrogen gradient used to power the synthesizing of ATP Difference in concentration in between cristae Chemiosmotic phosphorolation |
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phosphate is added to ADP yielding ATP NADH FADH |
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10 steps 1-5 endergonic 6-10 exergonic make ATP NADH cytoplasm for 1 glucose + phospate yields: -2 ATP -2 NADH -2 Pyruvate (Glucose snaps in half) -Carbons |
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Krebs Cycle: Purpose: location: |
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To make NADH FADH Matrix of mitochondria 2 turns=1 glucose --> 2 ATP, 6 NADH, 2 FADH, 4CO2 1 turn= 1 piruvate --> 1 ATP 3 NADH 1 FADH 2CO2 |
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Most ATP is made here cristae/inner membrane of mitochondria Chemiosmosis oxidative phosphorolation powers synthesis of ATP active transport through proteints fascilitative diffusion in atp synthase NADH is reduced protien is oxidized hydrogen is pumped into the intermembrane highly acidic then goes down the atp synthase fascilitated diffusion oxygen with cyt waits to bond with hydrogen so hydrogen gradient can be preserved. 34 ATP is made with H20 as producs |
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Krebs cycle does not like glucose. |
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Pyruvate is processed before going into the Kreb's cycle into what? |
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Pyruvate is stripped of carbon and releases CO2 modified by coenzyme A (CoA) in the matrix formingacetyl CoA this goes into the next cycle |
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Glycolysis Takes place? what's broken down? Purpose: Products: Process: |
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Cytoplasm Glucose produce pyruvate 2 pyruvate 2 ATP 2 NADH Substrate phosphororylation |
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Kreb's cycle: Takes place? what's broken down? Purpose: Products: Process: |
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Definition
Matrix 2 pyruvate to acetyl Coa reduce NAD FAD to NADH FADH 6NADH 2 ATP 2 FADH 4CO2 Substrate level phosphorolation |
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ETC Takes place? what's broken down? Purpose: Products: Process: |
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Cristae Inner Membrane NADH FADH Make ATP ATP water Oxidative Phosphorylation chemiosmosis |
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