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Allows you to see microscopic beings that aren't visible to the naked eye |
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Microscopes use lenses to make objects appear larger |
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Allows you to see fine details and gives you the ability to distinguish two objects that are close together as being two objects. |
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Objects absorbtion and transmission of light so some appear darker and some appear lighter |
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A technique used to enhance contrast |
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Stereomicroscope Viewing Range |
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a few hundredths of a millimeter to about one centimeter |
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Distance between your eyes on the microscope |
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Left switch is upper light and right switch is lower light |
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Used to view opaque (non-transparent) objects |
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Used to view transparent objects |
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Increases or decreases the apparent size of the object in view |
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On left eyepiece of Nikon Microscope and allows you to precisely adjust the sharpness of the image |
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Contains the 4 objectives and can be rotated to change objectives
(4X, 10X, 40X, 100X objectives)
(Shortest to Longest) |
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Product of eyepice and objective lens magnifications
(Eyepiece magnification=10X) |
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Moves the slide.
Lower Knob=moves slide left and right
Upper knob=moves the slide towards or away from you |
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Only used with 4X and 10X objective
moces the stage up or down quite a distance |
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Used with any magnification objective
Moves the stage vertically only a small distance |
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Contains an iris diaphragm |
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Can be opened to permit m ore light to illuminate the slide |
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Size of Human Cheek Cell/Amoeba |
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Magnifies up to 30X
Used to view specimens from a few hundredths of a mm to one cm
Used for things you can usually already see with the naked eye, but want to see in more detail
Allows you to see specimen in 3D |
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Term
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Definition
Light Switches, Base, Eyepiece, Magnification Control Knob, and Focusing Knob (increase resolution) |
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Nikon (Compound) Microscope |
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Definition
Allows magnification up to 1000X
Used for specimens smaller than .1 mm to a couple mm
Normally specimen is not visible to the naked eye
Specimen needs to be extremely thin so light can pass through, but this gets rid of 3D shape |
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Power switch, light source, condensor with diaphragm, fine focus, course focus, stage controls, mechanical stage, caliper, nosepiece, objectives, eyepiece with pointer, eyepiece with diopter |
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One major way to separate and identify proteins |
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Electrophoretic Separation |
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Depends on the net electrical charge of proteins |
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If a protein has a negative net charge... |
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Definition
it will migrate to the positive pole of the field |
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If a protein has a positive net charge... |
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Definition
it will migrate to the negate pole of the field |
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If a protein has no net charge... |
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it won't go toward either field |
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5 Amino Acids with Ionic R Groups |
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Ionic R group=charged
Aspartic acid, glutamic acid, lysine, arginine, and histidine |
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at some point on pH scale where a protein will have no net charge |
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Property that you change or manipulate in a systematic way
~the pH of the buffer (4,6,8) |
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Outcome affected by the independent variable
~electrical charge on protein |
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Graph of pH and Net Charge on Protein |
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Definition
Inverse relationship between pH of buffer and charge of protein (Downward slope to the right)
Higher pH=lower net charge or more negative |
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Property that Influences Protein Migration |
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Net electrical charge of protein; ionic R group is responsible for this property |
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H+ ion concentration decreases
H+ ions stick on proteins and cause it to have a positive charge; lower pH leads to higher H+ concentration |
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How fast solutes cross a membrane |
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ability of a molecule to pass through a cell's membrane |
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molecule is unable to pass through a cell's membrane |
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Red Blood Cell Plasma Membrane |
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relatively simple;composed largely of phospholipids with very few proteins |
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Dependent Variable in Cell Membrane Permeability Lab |
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Independent Variable in Cell Membrane Permeability Lab |
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Size, charge, lipid solubility |
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Time required to reach one-half the maximum value indicated by the plot |
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Spectrometer Instructions fro Membrane Permeability |
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Definition
1. Without any blank in the holder, turn the left knob to 0% transmittance which is 100% absorption
2. Put the blank in and turn the right knob to 100% transmittance which is 0% absorption
Transmittance=top numbers (right knob)
Absorption=bottom numbers (left knob)
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Right knob; amount of light that passes through; upper number reading on the scale |
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Left knob; amount of light absorbed; lower number reading on the scale |
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Not permeable (no lysis); ex: sodium and chloride ions |
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As size increases, the molecule becomes less permeable (inverse relationship); Ex: Urea, Glycerol, Glucose |
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As lipid solubility increases, the molecule becomes more permeable (direct relationship~increasing slope to the right); more CH or nonpolar bonds=more permeable
*Lipid solubility "trumps" size! |
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time it takes to lysis - starting point (bigger #)+ starting amount = H50
2 |
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As the size of a molecule increases... |
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As solute concentration increases... |
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water potential decreases |
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As molecular weight increases... |
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When the cells burst from having too much water diffused into them; when the solution becomes clear |
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As lipid solubility increases... |
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permeability increases
*direct relationship |
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Similar characteristics to Eukaryotes; live in hostile environments such as salty, hot, or acidic |
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Most familiar Prokaryote; can be heterotrophic or autotrophic; cell walls contain peptidoglycan distinguished by 3 different shapes |
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Organism that feeds on other things; aerobic=requires oxygen; anaerobic=does not require energy |
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self-feeders; ones that acquire energy from light=photosynthetic autotrophs |
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Nuclei bounded by nuclear membrane; diplomads, rhizaria and amoebozoans, euglenids and kinetoplastids, alveolata, stramenopila, rhodophytes, and slime molds |
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simple protist that lacks mitochondria but have 2 nuclei, move by way of flagella, looks like a face; heterotroph
GIARDIA |
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Move by way of pseudopodia which are stiff and project out to catch food; heterotroph
RADIOLARA |
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Move by way of pseudopodia which extend, reach out, attach, and pull an amoeba along; allow amoeba to engulf food particles from environment; heterotroph
AMOEBA PROTEUS |
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Move by way of flagella; some are photosynthetic; autotroph
EUGLENA |
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Move by way of flagella; have one large mitochondrion that contains kinetoplast; kinetoplast contains DNA and proteins that help with mitochondrial function; autotroph
TRYPANOSOMA (sleeping sickness) |
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Move by way of cilia; unicellular; specialized regions for feeding, excretion, and locomotion; heterotrophic
PARAMECIUM |
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disease causing internal parasites of animals; unicellular; heterotrophic
PLASMODIUM |
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unicellular; photosynthetic (chlorophyll a and c); autotrophic; moves by way of flagella; "shells" of cellulose and silica give it it's shape
PERIDINIUM |
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move by way of 2 flagellas; phosynthetic (cholorphyll a and c); autotrophic; unicellular; silica/calcium in cell wall
DIATOMS
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photosynthetic (chlorophyll a and c); multicellular; autotrophic
SARGASSUM and FUCUS |
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seaweeds; autotrophic; photosynthetic (cholorphyll a and d)
BATRACHOSPERMUM |
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heterotrophic (take in food by phagocytosis); acellular; sometimes produces spores; multinucleated
PHYSARUM |
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heterotrophic; cell walls made of chitin; multinucleate
zygomycota, basidiomycota, ascomycota
ALL END IN -MYCOTA |
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Definition
reproduce sexually by having the hyphae of adjoining individuals extending branches taht make contact, coalesce, and form gametes that fuse together to form a zygote that then can develop into an individual
RHIZOPUS |
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Basidiomycota (The Club Fungi) |
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reproduce sexually via basidia
Ex: mushrooms you see walking through the woods
CLUB MUSHROOM |
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Ascomycota (The Sac Fungi) |
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Definition
very diverse group; heterotrophic; reproduce sexually via asci structures that contain spores
SACCHAROMYCES (YEAST) |
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