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Biology Lab Pratical
For all Gannon Biology lab practicals
93
Biology
Undergraduate 1
10/04/2010

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Term
Ocular (Eyepiece)
Definition
Pair of lenses nearest the eyes, magnify 10X
Term
Body Tube
Definition
Supports the oculars and revolving nosepiece
Term
Revolving Nosepiece
Definition
A rotating structure with four objectives mounted on it.
Term
Objectives
Definition
Lenses near the specimen. Low (4X), Medium (10X), High (40X), Oil (100X)
Term
Mechanical Stage
Definition
Horizontal plate with slide holder
Term
Slide Adjusting Knob
Definition
Moves the slide holder up, down, left and right
Term
Substage Condenser
Definition
A system of lenses that concentrates the light from the illuminator onto the specimen
Term
Iris Diaphragm
Definition
Regulates the amount of light
Term
Illuminator
Definition
Provides the source of light
Term
Arm
Definition
Supports the body tube and adjustment knobs
Term
Adjustment Knob
Definition
Move mechanical stage closer and further from objectives. Fine and coarse knobs
Term
Base
Definition
Bears the weight of the microscope
Term
Magnification
Definition
Is how much large the objective appears compared to its real size
Term
Total Magnification
Definition
Power of the Ocular X Power of the Objective
Term
Resolution
Definition
A measure of the clarity of the image
Term
Field of View
Definition
The circular field you see when you look through the oculars
Term
Depth of Focus
Definition
Perception of depth by focusing up and down
Term
Inversions
Definition
The phenomena that the object and its movements are inverted
Term
Working distance
Definition
Space between the slide and the objective in use
Term
Parfocal
Definition
Unnecessary to make a major change in the focus when magnification is changed
Term
Plasma (Cell) Membrane
Definition
Functions as a selective barrier for all cells
Term
Cytoplasm
Definition
Suspension of cytosol and organelles
Term
Cell Wall
Definition
Rigid transparent structure made of mostly cellulose
Term
Central Vacuole
Definition
- Vital to the elongation of plant cells
- Occupies 90% of mature cells
Term
Chloroplasts
Definition
An organelle containing enzymes and photosynthetic pigments (ex. chlorophyll) that function in the production of sugar by photosynthesis
Term
Red Blood Cells (Erythrocytes)
Definition

Most numerous, shaped like a pink donut with no nucleus

[image]

Term
White Blood Cells (Leukocytes)
Definition
Less numerous, larger than red blood cells with a uniquely shaped nucleus, stained purple
Term
Platelets
Definition
No nuclei, purple-grey specks scattered between red and white blood cells
Term
Organic Compound
Definition
Molecules containing carbon in the shape of chains or rings
Term
Carbohydrates
Definition
Made of carbon, hydrogen and oxygen. Includes starches, which store energy
Term
Proteins
Definition
Large molecules made of amino acids.
Term
Amino Acids
Definition
Made of Carbon, Oxygen, Hydrogen and Nitrogen. Carboxyl, Amino and an R group.
Term
Lipids
Definition
Fatty and oily substances that are insoluble in polar substances (ex. water) Composed of carbon, oxygen and hydrogen
Term
Nucleotides
Definition
Five-carbon sugar, nitrogenous base and a phosphate group
Term
Dehydration Synthesis
Definition
Bond resulting from the creation of a water molecule
Term
Hydrolysis
Definition
Dissolution of a bond with the addition of water between two molecules
Term
Reducing Sugar
Definition
Sugars with the ability to add electrons to other molecules due to free carbonyl groups near hydroxyl groups
Term
Benedict's Reagent
Definition
An alkaline solution containing copper ions used to test for reducing sugars. Blue colored. Positive results are green (low concentration) to brown (high concentration)
Term
Lugol's Reagent
Definition
Used to detect starches. Iodine molecules unravel glucose polymers to create a dark blue/purple to black color.
Term
Biuret Test
Definition
A strong solution of NaOH or KOH ions and dilute CuSO4 used to detect proteins. Positive results are violet.
Term
Enzymes
Definition
Proteins that act as catalysts to cause substrates to form products.
Term
Amylase
Definition
An enzyme that recognizes the substrates amylose and glycogen.
Term
Rennin (Rennilase)
Definition
Enzyme that converts casein into the shorter protein chained paracasein, leaving curd precipitate and whey
Term
Sudan IV
Definition
Powdered reagent that is lipid soluble. Stains lipids red.
Term
Emulsifier
Definition
A substance which stabilizes an emulsion by increasing its kinetic stability
Term
Diffusion
Definition
The process by which molecules move down a concentration gradient from high to low concentration
Term
Selectively (Differentially) Permeable
Definition
Allows passage of a restricted set of molecules. Large and polar molecules do not pass through easily
Term
Solvent
Definition
Dissolves a substance in a solution
Term
Solute
Definition
The substance that is dissolved in a solution
Term
Osmosis
Definition
The diffusion of water from low concentration (high concentration of water) to high concentration (low concentration of water)
Term
Osmotic Pressure
Definition
The force that causes osmosis
Term
Isotonic
Definition
Equal concentrations. No net gain or loss
Term
Hypotonic
Definition
A low concentration solution. Causes a cell to gain water
Term
Hypertonic
Definition
A high concentration solution. Causes a cell to lose water
Term
Brownian Movement
Definition
Movement due to bombardment by fast-moving molecules
Term
Concentration Gradient
Definition
The gradual differences over the distance between high and low regions of concentration. The steeper the gradient, the faster the diffusion
Term
Effects on the Rate of Diffusion
Definition
Rate of diffusion is:
Inversely proportional to molecular weight
Directly proportional to temperature
Term
Colloid
Definition
A suspension of large, insoluble particles in a fluid medium
Term
Phenolphthalein
Definition
pH indicator. Turns pink-red in basic solutions and green-blue in acidic solutions
Term
Crenation
Definition
The shrinking or shriveling of cells
Term
Hemolysis
Definition
The swelling, and eventually bursting, of animal cells
Term
Total Solute Concentration
Definition
When plasmolysis occurs in 50% of cells in a solution
Term
Plasmolysis
Definition
When a plant cell shrinks and the plasma membrane breaks away from the cell wall
Term
Hydration Shells (Spheres)
Definition
The group of water molecules oriented around an ion
Term
Prokaryotic Cells
Definition
- Unicellular
- Reproduce by binary fission
- 1 strand DNA in ring form in Nucleoid Region
- Lack: Nuclear envelope, Chromosomal proteins, Membrane-bound organelles
Term
Plasmids
Definition
Smaller rings of DNA that perform specialized functions. Present in prokaryotes
Term
Bacteria
(Classifications)
Definition
Bacillus (Rod)
Coccus (Spherical)
Spirillum (Spiral)
Term
Peptidoglycan
Definition
Present in cell walls. Provides protection and maintains shape. Not present in Archaea
Term
Autotrophs
Definition
Require carbon dioxide
Term
Phototrophs
Definition
Require light energy
Term
Heterotrophs
Definition
Require at least one organic compound
Term
Chemotrophs
Definition
Require chemicals from environment
Term
Photoautotrophs
Definition
Require light and carbon dioxide. Includes cyanobacteria
Term
Chemoautotrophs
Definition
Require inorganic sources (nitrogen, iron, sulfur) and carbon dioxide
Term
Photoheterotrophs
Definition
Require organic carbon and light
Term
Chemoheterotrophs
Definition
Require other organisms for carbon and energy. Includes most bacteria
Term
Neutralization Reaction
Definition

HCl + NH4OH -> NH4Cl + H2O

N+ + Cl- + NH4+ + OH-

Term
Diffusion of Gas
Definition
[HCl NH4OH] Reaction occurs near HCl because Cl- is heavier
Term
Spirilla (Shapes)
Definition

Single cells

[image]

Term
Baccili (Shapes)
Definition

Pairs, chains

[image]

Term
Cocci (Shapes)
Definition

Pairs (Diplococci)

Chains (Streptococci) Strep throat

Clusters (Staphlococci) Staph infection

[image]

Term
Gram staining
Definition
Positive stain=Pink, simpler, more peptidoglycan

Negative stain=Purple, complex, less peptidoglycan
Term
Nitrogen fixation
Definition
Conversion of N2 to nitrate or organic nitrogen. Used by many bacteria and cyanobacteria
Term
Extremophiles
Definition
Archaea that can in exist in extreme environments
Term
Cyanobacteria
Definition
- Exist mostly as colonies and filaments
- Simple nutritional requirements
- Include Oscillatoria and Nostoc
Term
Oscillatoria
Definition
- Divide in the same plane and adhere after division, resulting in a long filament of cells[image]
Term
Nostoc
Definition

- Forms grape-like colonies

- Vegetative cells: Smaller

- Heterocysts: Larger, nitrogen fixation

[image]

Term
Mixotrophs
Definition
Use a combination of autotrophic and heterotrophic means to gain nutrition
Term
Protists
Definition
- All eukaryotic
- Primarily asexual, possible sexual
Term
Euglena
Definition

- Pellicle: Allows body to change shape

- Movement by flagellum

- Mixotrophic

[image]

Term
Protozoa
Definition

- Heterotrophic

- Unicellular

- Organelles

Term
Amoebas
Definition

- Movement by pseudopodia

- Heterotrophic

[image]

Term
Ciliates/Paramecium
Definition

- Movement by cilia

- Most advanced protozoa

- Asexual by binary fission, sexual by conjugation

[image]

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