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the ratio of an object’s image size to its actual size |
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magnification, resolution & contrast |
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measure of the clarity of an image as a result of magnification |
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the minimum distance two points can be separated and still be distinguished as two unique points |
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measure of the distinguishability of different parts of a sample |
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scientists stain parts of samples different colors to increase this |
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light microscope, scanningelectron microscope , transmission electron microscope |
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passes visible light through a specimen and then through glass lenses which refract the light and magnify the image maximum magnification: 1000× resolution limit: 200 nanometers |
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scanningelectron microscope (SEM) |
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focuses a beam of electrons across the surface of a specimen gives a 3-dimensional image of the surface of the specimen can only be used on dead or non-living samples resolution limit: 2 nanometers – 0.002 nanometers |
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transmission electron microscope (TEM) |
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focuses a beam of electrons through a specimen gives extremely detailed images of the interior of cells can only be used on dead or nonliving specimens resolution limit: 2 nanometers – 0.002 nanometers |
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EMs
- more detailed
- allow to see within a cell's interior
- allow to see outer surface
LMs
- cheaper
- faster to use
- observe living cells
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What is the difference between Light Microscopes and Electron Microscopes?
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separates major cell organelles and subcellular structure to be studied |
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an instrument that spins samples at extreme speeds to separate the contents of the cell |
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prokaryotes & eukaryotic cells |
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defined by the lack of internal membrane structures typically smaller than eukaryotes exist as relatively simple unicellular organisms (simpler than eukaryotes, but still complex enough for life) includes: bacteria and archaebacteria |
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defined by the presence of internal membrane structures large, complex cells can possibly form multicellular organisms includes: animals, plants, fungi, and protists |
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internalmembrane structures |
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Note: Current debate ensues on how to classify protists, and some scientists do not consider them eukaryotes, but they do have |
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Note: Bacteria and archaebacteria are two completely different |
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outer membrane present for ALL cells (both eukaryotes and prokaryotes); internal membrane structures only exist in eukaryotes (These notes apply to all membranes, not just the outer membrane.) |
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composed of the lipid bilayer |
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phospholipids with hydrophobic fatty acid tails and hydrophilic phosphate heads |
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a semi-permeable membrane |
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certain small molecules can diffuse through larger molecules can enter through specific membrane protein channels |
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molecules tend to move from areas of higher concentration to areas of lower concentration membranes can be used to establish these areas of concentration and either maintain or change them |
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membrane embedded protein with a carbohydrate attached to it used in cell-to-cell communication |
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can increase fluidity of a membrane |
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are more fluid than saturated fats because of the kink in the fatty acid chain |
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plasma membranes contain copious amounts of |
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“fluid” because individual molecules that make up the protein move around; “mosaic” because membrane is made of a collection of phospholipids, proteins, and other large molecules |
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how does the fluid mosaic model get its name? |
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occurs at a maximum surface area to volume ratio |
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