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Biology 400 Final
Info from Test #3
19
Biology
Undergraduate 2
12/16/2010

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Term
Definition: Catabolism and Anabolism
Definition
Catabolism: breaks down complicated molecules to release energy
Anabolism: builds complicated molecules, requires energy
Term
The Anabolism of Histidine
Definition
10 steps from ammonia and glycerol derivative
Histidine is its own noncompetitive inhibitor
Negative feedback at first step saves energy
ADP also inhibits, as more ATP is changed to ADP, it binds to first enzyme and inhibits
Term
The Anabolism of phenylalanine, tyrosine, and tryptophan
Definition
All made by the same pathway
PEP>A>B>chorismate
(from chorismate) --> C>D>Phe>Tyr
(also from chorismate) --> E>F>Trp
Can't inhibit Trp at first enzyme, would then stop making Phe and Tyr
Can inhibit first enzyme after chorismate
Each amino acid also weakly inhibits the first enzyme (though not completely)
Term
Major catabolic processes (and equation)
Definition
C6H12O6 + 6O2 --> 6CO2 + 6H2O

1. Glycolysis - net 2 ATP, 2 NADH, takes 10 steps to convert glucose to 2 pyruvates
2. Citric Acid Cycle - net 2 ATP, 2 NADH, 2 FADH2; converts 2 pyruvate to 6CO2
3. Electron Transport Chain - electron transport + oxidative phosphorylation converts 6CO2 to 34 ATP
Term
What is NAD?
Definition
Nicotinamide Adenine Dinucleotide
Oxidized form = NAD+
Reduced form = NADH
Term
What is FAD?
Definition
Flavin Adenine Dinucleotide
Oxidized form = FAD+
Reduced form = FADH2
Term
Definition: Electron Transport Chain
Definition
Uses NADH and FADH2 to pump H+ ions against gradient
The concentration of H+ gradient pushes the ions back into the cell through ATP synthase which produces ATP
Term
Microbial diversity of the ETC
Definition
Carbon sources:
Autotroph=uses CO2 (plants)
Heterotroph=uses C from other compounds (humans)
Energy Source:
Phototroph=uses light (plants)
Chemotroph=uses oxidation (humans)
Electron source:
Lithotroph=inorganic (plants)
Organotroph=organic (humans)
Term
Steps of ETC:
Definition
1. Electrons flow to acceptors
2. Protons pump out of cells, make proton gradient
3. ATP synthase allows protons to flow back into cell
4. ATP is made
Term
Steps of ETC in E. coli
Definition
1. NADH moves two electrons to NDH-1 (4 protons move to periplasm)
2. NDH-1 moves two electrons to quinone (2 protons moved to periplasm)
3. Quinone moves two electrons to cytochrome bo
4. Cytochrome bo moves two electrons to oxygen
Term
Steps of ETC in mitochondria
Definition
1. Complex 1 passes e's from NADH to coenzyme Q (4 H+ moved)
2. E's move from coenzyme Q to complex III
3. E's move from complex III to cytochrome c (4 H+'s moved)
4. e's move from cytochrome c to Complex IV (2 H+'s moved)
5. e's move from Complex IV to Oxygen --> makes water
Term
Photosynthesis in Purple Photosynthetic Bacteria
Definition
Uses bacteriochlorophyll P870 - infrared, therefore less energy
Does NOT produce water from oxygen
P870 is an e- donor and acceptor on Photosystem II
Term
Photosynthesis steps of Purple Photosynthetic Bacteria
Definition
1. Light excites an e- to Photosystem II
2. 2 e-'s are donated to quinone, takes 2H+'s with it
3. Quinone becomes quinol
4. e-'s move to cytochrome bc with 2 H+'s
5. e-'s move to cytochrome c
6. e-'s return to Photosystem II
This is a CYCLIC process.
Term
Photosynthesis Steps of Cyanobacteria
Definition
Uses Photosystem II (P680) and Photosystem I (P700)
These are higher energy than Purple photosynthetic bacteria, therefore CAN make water from oxygen
1. 4 photons excite 4 e-'s from 2 H2Os
2. e-'s moved to 2 quinones (becomes quinol)
3. e-'s move to cytochrome bf
4. Plastocyanin moves e-'s to Photosystem I
5. Light excites the e-'s which move to NADP+ and ferredoxin
Term
Cost of the Dark/Calvin cycle in Cyanobacteria
Definition
To make one glucose:
6 CO2
18 ATP
12 NADPH
Term
Where do glycolysis, the Krebs cycle, and the Electron Transport Chain take place in a cell?
Definition
Glycolysis takes place in the cytoplasm
The Krebs cycle takes place in the inner matrix of mitochondria
ETC takes place in the membrane of mitochondria
Term
Overview of Glycolysis
Definition
Costs 2 ATP to produce 4 ATP (2 net), 2 NADH and 2 pyruvate
Total of 10 steps (first 5 are energy activating, last 5 are energy harvesting)
1. Glucose activated with ATP
2. Glucose split in half
3. Remaining halves are oxidized
Term
10 steps of Glycolysis with products
Definition
1. Hexokinase adds a phosphate from ATP (Cost: -1 ATP)
2. Glucose isomerases and moves a C-O bond
3. Phosphofructokinase phosphorylates (Cost: -1 ATP)
4. Aldolast splits the molecule into Glyceraldehyde-3-Phosphate and Dihydroxyacetone-3-Phosphate
5. Triosephosphate isomerases, now two of the same molecule
6. G3P-Dehyrogenase oxidizes the 1-Carbon (Product: +2 NADH)
7. Phosphoglycerate kinase removes a phosphate and adds it to ADP (Product: +2 ATP)
8. Phosphoglyceromutase moves remaining phosphate to middle C
9. Enolase removes water (Product: +2 H2O)
10. Pyruvate kinase removes a phosphate and adds it to ADP (Products: +2 ATP, +2 pyruvate)
Term
Structure of Pyruvate:
Definition

OH

   [

   C=O

   [

   C=O

   [

H-C-H

   [

   H

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