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2 Prokaryotes
May 29 Class 14
27
Biology
Undergraduate 2
05/18/2015

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Term
How does a eukaryotic promoter allow a gene to
express in a tissue-specific manner?
Definition
Simplest model – there is a transcription factor that
binds there and tells it to express.
Term
How is a protein specifically expressed in only one place?
Definition
Combinations
No one factor has to be tissue-specific, but a given combination may only appear in a single tissue type.
Term
Give a solution on how using combinations will be the solution to sending a eukaryotic promoter to the liver.
Definition
The liver will have a liver specific promoter, but there will be enhancers that match the combination of transcription factors only present in the correct tissue
*each tissue has its own specific combination
Term
What do the linear sequences of bases in DNA (and the consequent sequence of amino acids in protein) direct?
Definition
how everything works
where and when everything works
Term
What do the linear sequences of bases in DNA (and the consequent sequence of amino acids in protein) reveal?
Definition
evolutionary relationships
Term
How is it possible that a human, gibbon, and rhesus monkey are related?
Definition
Least common ancestor
Term
Which is a human closer related to, and why? a gibbon or a rhesus monkey?
Definition
A gibbon, due to DNA sequence. They are more similar.
Term
How long has it been since rotifers have had sex? What allows them to do this?
Definition
No sex for 80 million years.
They evolve by scavenging DNA from environment.
Term
What are the 2 groups that consist of prokaryotes?
Definition
Bacteria and Archaea
Term
What are some characteristics of prokaryotes?
Definition
No membrane-bound organelles
Cell wall
0.5-5 um diameter
DNA in nucleoid region
Term
How are bacteria characterized?
Definition
Shape, cell wall, flagella, capsule, fimbriae
Term
What are the three shapes of bacterial?
Definition
Spherical
Rod-shaped
Spiral
Term
Describe the cell difference in bacteria
Definition
Gram positive=peptidoglycan traps crystal violet
Gram negative=crystal violet is easily rinsed away, revealing red dye
Term
What two things about flagella are ways that prokaryotes can use them to be characterized by?
Definition
Number
Location
Term
What does the capsule in bacteria do?
Definition
allow bacteria to attach to their substrate
Term
What is a substrate?
Definition
an inert surface or a target cell
Term
What does the Fimbriae do?
Definition
allow bacteria to contact each other or a surface
Term
Describe Endospores
Definition
Resistant cell bodies formed during stress
Copy of genome surrounded by membrane, with almost all water stripped out
Allow long-term survival in adverse conditions
Term
How long can endospores survive?
Definition
8,000 (frozen in glacier)
750,000 (frozen in Antarctic ice)
30,000,000 years (from gut of bee entrapped in amber)
250,000,000 years (from water droplet trapped in salt crystals)
Term
What are two nutritional modalities that prokaryotes use?
Definition
Energy source
Carbon source
Term
What are three ways in which prokaryotes use oxygen?
Definition
Obligate aerobe-requires oxygen
Obligate anaerobe-poisoned by oxygen
facultative anaerobes-can use oxygen (or not)
Term
What is an obligate aerobe?
Definition
Prokaryotes that require oxygen
Term
What is an obligate anaerobe?
Definition
Prokaryotes that are poisoned by oxygen
Term
What is a facultative anaerobe?
Definition
Prokaryotes that can use oxygen (or not)
Term
What is Nitrogen fixation?
Definition
Conversion of N2
to NH3
- NH3 can be used for amino acid synthesis
Can make nitrogen available to plants
- Allows them to grow in nitrogen-deficient soil
Term
What is Metabolic cooperation?
Definition
Where colonies of bacterial cells cooperate
Term
How do cells cooperate in metabolic cooperation?
Definition
Specialization, cooperation
-Channels allow exchange of materials
-Chemical signals released for recruitment, communication
-Some Cyanobacteria
-Biofilms
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