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Biology 102 Lab Practical
Notes from Thursday 9AM slides
235
Biology
Undergraduate 1
04/27/2010

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Term
what are the two types of cells with structural differences?
Definition
prokaryotes and eukaryotes
Term
what organisms are prokaryotes?
Definition
bacteria
Term
how are prokaryotes structural unique?
Definition
non-membrane bound organelles, peptidoglycan cell wall
Term
what organisms are eukaryotes?
Definition
animals and plants
Term
how are eukaryotes structurally unique?
Definition
nucleus, membrane bound organelles
Term
true/ false: all cells have a plasma membrane
Definition
true
Term
what does the plasma membrane regulate?
Definition
what enters and leaves the cell
Term
how do molecules cross the plasma membrane?
Definition
diffusion and osmosis
Term
the process by which molecules spread from areas of high concentration to areas of lower concentration
Definition
diffusion
Term
the diffusion of water molecules through a selectively permeable membrane
Definition
osmosis
Term
osmosis will continue as long as there are more water molecules on one side of the membrane
Definition
osmotic pressure
Term
osmosis will continue to diffuse until there are equal numbers of molecules inside and outside the cell
Definition
osmotic balance
Term
-same solute concentration in and out
-no net water flow-cells remain the same size
Definition
isotonic
Term
-more solute outside-water flows out-cells shrink
Definition
hypertonic, hyper kids pee in plants
Term
the shrinking of cytoplasm of a cell in response to diffusion of water out of the cell
Definition
plasmolysis, saline solution
Term
-more solute inside-water flow in-cells bloat-turgid
Definition
hypotonic
Term
the capacity to do work
Definition
energy
Term
types of cellular work
Definition
mechanical, transport, chemical
Term
energy must be added prior to the formation of products
-reactants (also referred to as substrates) are joined after energy is added
Definition
endergonic reactions
Term
example of mechanical cellular work
Definition
beating of cilia
Term
example of transport cellular work
Definition
pumping of molecules and ions across the plasma membrane
Term
example of chemical cellular work
Definition
pushing endergonic reactions that would not occur spontaneously
Term
-energy is released following the formation of products
-Reactants (substrates) are joined and energy is given off
Definition
exergonic reactions
Term
states of chemical reactions
Definition
initial :reactants/ transitional: instability, bonds broken/ final: products
Term
the input of energy needed to get from the initial state to the transition state
Definition
activation energy
Term
what does an enzyme do?
Definition
lowers the activation energy, which speeds up the reaction
Term
enzymes are typically?
Definition
proteins
Term
where do substrates (reactants) bind to on an enzyme?
Definition
the active site
Term
a small conformational change that occurs so that the active site fits even more snuggly around the substrate
Definition
induced fit
Term
enzymes are what specific?
Definition
substrate
Term
are enzymes consumed during a reaction?
Definition
no
Term
what three things effect enzyme activity?
Definition
temperature, pH levels, and inhibitors
Term
excess heat can break down the bonds that maintain an enzyme’s structure
Definition
denaturation, can be reversed sometimes -> renaturation
Term
bind to the active site and block the substrate from entering
Definition
competitive inhibitor
Term
bind in a different place from the active site but cause a structural change to the enzyme and the active site
Definition
noncompetitive inhibitor
Term
enzymes that perform the same function, but are slightly different and have different optimal temperatures and/or pH environments
Definition
isozymes
Term
how does iodine indicate the presence of starch
Definition
it turns blue/black, slides into starch coil to give color
Term
Nutrients, such as Nitrogen, Phosphorous and Ammonia can be transferred from oceans to land (including intertidal pools) by way of seabird
Definition
seabird guano
Term
how is bird guano good for plants?
Definition
Nitrogen and Phosphorous are important nutrients for plant growth
Term
how is bird guano bad for plants?
Definition
Ammonia can inhibit seed germination
Term
the sum of all chemical processes occurring within an organism
Definition
metabolism
Term
production of molecules for building tissues such as muscle and bone
-requires energy (ATP)
Definition
anabolism
Term
the breaking down of molecules into various components
-release of energy from the breaking of chemical bonds
Definition
catabolism
Term
the process by which cells generate ATP from the energy stored in glucose
Definition
oxidation
Term
two major modes of generating ATP
Definition
cellular respiration, fermentation
Term
aerobic: uses oxygen
-generates 36-38 ATP
-three parts
Definition
cellular respiration
Term
three parts of cellular respiration
Definition
-glycolysis: 2 ATP
-Citric acid cycle: 2 ATP
-Oxidative Phosphorylation: 32-34 ATP
Term
-anaerobic: doesn’t use oxygen
-generates 2 ATP
-2 kinds of fermentation
Definition
fermentation
Term
two types of fermentation
Definition
lactic acid fermentation, alcohol fermentation
Term
lactic acid is the byproduct
Definition
lactic acid fermentation
Term
ethanol and CO2 are byproducts
Definition
alcohol fermentation
Term
glucose is broken down into pyruvate
Definition
glycolysis
Term
when does glycolysis happen
Definition
cellular respiration, fermentation
Term
how much ATP does glycolysis produce?
Definition
2
Term
a minute life form; any organism of microscopic size
Definition
microbes
Term
require O2 for growth
-grow close to the loose fitting cape
Definition
obligate aerobes
Term
growth ceases in presence of O2
-grow farthest away from loose fitting cap
Definition
obligate anaerobes
Term
both aerobic and anaerobic growth
-growth throughout tube
-more growth closer to loose cap
Definition
facultative anaerobes
Term
-facultative anaerobes
-prefer an O2 rich environment
-switch to fermentation if no O2 is present
Definition
yeast
Term
-its enzymes are able to metabolize sugars via alcohol fermentation
-produce CO2, which causes dough to rise
Definition
baker's yeast
Term
also breaks down sugars via alcohol fermentation
-produces ethanol and CO2
Definition
brewer's yeast
Term
three different sugars used in lab
Definition
-glucose
-maltose
-fructose
Term
use amount of CO2 present in lab to
Definition
measure metabolic activity
Term
two factor experimental design
Definition
ANOVA
Term
-“self-feeders”
-are able to transform inorganic carbon (CO2) into organic compounds such as glucose
-are the producers of the biosphere
Definition
autotrophs
Term
-“other-feeders”
-must ingest organic forms of carbon (i.e. other organisms)
-are the consumers of the biosphere
Definition
heterotrophs
Term
two types of autotrophs
Definition
chemoautotrophs, photoautotrophs
Term
use energy from reactions of inorganic compounds
Definition
chemoautotrophs
Term
-use sunlight as their energy source via a process of photosynthesis
-use light energy to drive synthesis of organic molecules from carbonic dioxide and water
Definition
photoautotrophs
Term
-inorganic carbon (which cannot be used to generate ATP) is converted into organic carbon (glucose)
-occurs in plants, algae, and in some bacteria and protists
Definition
photosynthesis
Term
two parts of photosynthesis
Definition
light dependent reactions, light independent reactions
Term
-are carried out in thykaloid membranes
-within the chloroplasts
-pigments within capture light
-convert light energy into the chemical energy of ATP and NADHP
-split H2O and release O2 to the atmosphere
Definition
light dependent reactions
Term
-take place in the stroma
-use ATP and NADHP to convert CO2 to the sugar G3P
-return ADP, inorganic phosphate, and NADHP+ to the light dependent reactions
-Calvin cycle
Definition
light independent reactions
Term
CO2 from the atmosphere is captured and modified by the addition of hydrogen to form carbohydrates
Definition
calvin cycle, carbon fixation
Term
what does the calvin cycle use to operate?
Definition
occurs through the use of ATP and NADHP from light-dependent reactions
Term
Chloroplasts are believed to have evolved from
Definition
a symbiotic relationship between cyanobacteria and an eukaryotic cell
Term
light provides electrons for the production of ATP (used by the light-independent reactions)
Definition
light-dependent reactions
Term
use ATP (generated by light-dependent reactions) to make glucose
-example of anabolism
Definition
light-independent reactions
Term
used to separate a mixture into its component molecules
-molecules migrate up the paper at different rates
-due to differences in polarity
Definition
Photo Chromotography
Term
what moves up the fastest to slowest up chromotography paper
Definition
carotene
-Xanthropyll
-Chlorophyll a
-Chlorophyll b
Term
-are chain of more than 300 small volcanic islands
-nearly all are part of Alaska
-the Commander Islands belong to Russia
Definition
Aleutian Islands
Term
uses key species grown under controlled conditions to test the effects of specific variables on plant growth
Definition
Phytometer Experiment
Term
central ideas of evolution
Definition
has a history – has changed over time
-different species have common ancestors
Term
evolution with a single branch within the evolutionary tree
Definition
anagenesis
Term
an ancestral population splits into two new groups that evolve independently of one another
Definition
cladogenesis
Term
represents the evolutionary relationships among a set of organisms or groups of organisms called a taxa
Definition
phylogeny
Term
represent groups of descendent taxa (often species)
Definition
tips
Term
represent the common ancestor of the groups
Definition
nodes
Term
two descendents that split from the same node are called
Definition
sister groups
Term
a taxon outside the group of interest is called an
Definition
outgroup
Term
a grouping that includes a common ancestor and all the descendents (living and extinct) of that ancestor
Definition
clade
Term
clades can be
Definition
nested
Term
a group comprised of one ancestor and all its descendents
Definition
monophyletic group
Term
includes parts from different monophyletic groups
Definition
paraphyletic
Term
three domains of life
Definition
eukarya, archaea, bacteria
Term
two types of prokaryotes
Definition
archaea and bacteria
Term
what is the prokaryote mode of nutrition
Definition
all four modes
Term
prokaryotes are not
Definition
monophyletic
Term
two major structures of prokaryotes
Definition
peptidoglycan cell wall, circular chromosome
Term
true/ false: all protists have nucleus
Definition
true
Term
protist ways of eating
Definition
heterotrophic, autotrophic, and mixotrophic
Term
where do most protists live?
Definition
water
Term
any eukaryotic organism that is not a plant, animal, or fungus
Definition
protist
Term
are protists monophyletic?
Definition
no
Term
are protists monophyletic?
Definition
no
Term
types of locomotion used by protists
Definition
flagella, cilia, and pseudopods
Term
endiosymbiosis in protists
Definition
primary and secondary
Term
primary endosymbiosis
Definition
cyanobacteria + heterotrophic eukaryote
Term
chlorophytes
Definition
can be unicellular, colonial, filamentous, or multicellular
-all are aquatic
Term
charophyceans
Definition
-> origins of land plants
-are the green algae mostly related to land plants
Term
-> early vascular plants appear
- have gametangia
Definition
bryophytes
Term
house egg or sperm
-egg or sperm surrounded by a jacket of cells
Definition
gametangia
Term
seedless vascular plants
Definition
-> early seed plants appear
-vascular system
Term
->radiation of flowering plants, have seeds
Definition
gymnosperms
Term
have developing seed within an ovary
Definition
angiosperms
Term
haploid gametophytes and diploid sporophytes produce one another
Definition
alternation of generations
Term
haploid
-produce eggs and sperm that fuse to produce a diploid stage, the sporophyte
Definition
gametophyte
Term
diploid
-produce airborne spores via meiosis
-spores grow into the gametophyte generation
Definition
sporophyte
Term
most conspicuous stage in moss life cycle
Definition
haploid
Term
-gametophyte dominance
-sporophyte dependence
Definition
moss life cycle
Term
-reduction of gametophyte
-independence of sporophyte
Definition
fern life cycle
Term
-microscopic gametophytes
-protection by sporophyte tissues
Definition
seed plant life cycle
Term
plants produce one kind of spore, which usually gives rise to a bisexual gametophyte
Definition
homospory
Term
examples of homospory organisms
Definition
bryophytes and most pteridophytes
Term
have two kinds of spores
Definition
heterospory- mega and microsporangia
Term
produce megaspores that give rise to female gametophytes
Definition
megasporangia
Term
produce microsporophylls that give rise to male gametophytes
Definition
microsporangia
Term
examples our heterspory organisms
Definition
some lycopods (club mosses) and all seed plants
Term
life cycle stages
Definition
-diploid between fertilization and meiosis to make spores
-haploid after spore production till fertilization
Term
two kinds of seed plants
Definition
angiosperms and gymnosperms
Term
food reserves in gymnosperms
Definition
gametophyte tissue = n
Term
in gymnosperms seed coat
Definition
(derived from parent sporophyte) = 2n
Term
seeds are housed in fruits
Definition
receptacle seeds
Term
endosperm
Definition
in angiosperms -food supply
-3n -> triploid
Term
where are pollen grains found?
Definition
in the anther sac
Term
complete flower
Definition
all whorls
Term
incomplete flower
Definition
lacks one or more whorls
Term
whorls
Definition
which are rings of leaves
Term
how many whorls in a flower?
Definition
-1. The sepal on the outside
-2. The petals lying inside the sepal
-3. The stamens enclosed by the pedals
-4. The carpal in the center of the flower
Term
perfect flower
Definition
stamens and carpal
Term
imperfect flower
Definition
stamens or carpal
Term
flower types
Definition
solitary or inflourescence
Term
fruit types
Definition
fleshy or dry
Term
dry fruit splits at maturity
Definition
dehiscent
Term
remains closed at maturity
Definition
indehiscent
Term
fruit complexities
Definition
simple, aggregate, multiple
Term
simple fruit
Definition
one fruit, one ovary
Term
aggregate fruit
Definition
many ovaries from a single flower, raspberry
Term
many ovaries from many flowers
Definition
multiple fruit, pineapple
Term
flower plants that have two embryonic seed leaves, or cotyledons
-beans
-vascular tissue ring
Definition
eudicot
Term
monocot
Definition
flower plants that have one embryonic seed leaf, or cotyledons
-corn
-no vascular tissue ring, spread out
Term
fruit dispersal
Definition
-wings
-animal feces
-animal fur
Term
-alternation of generations with a multicellular sporophyte that is dependent on gametophyte resources
-spores
Definition
phylogeny between charophyceans and bryophytes
Term
between bryophytes and pteridophytes
Definition
sporophyte becomes independent of gametophyte
Term
between pteridophytes and gymnosperms
Definition
seeds
Term
endosperm/ fruits and flowers
Definition
between gymnosperms and angiosperms
Term
terrestrial adaptations that distinguish land plants from charophycean algae
Definition
apical meristems, multicellular dependent embryos, alternation of generations, multicellular gametangia
Term
two types of gametnagia
Definition
antheridia, archegonia
Term
archegonia
Definition
produces and retains egg cells
Term
antheridia
Definition
produces sperm
Term
-absorb water and nutrients
-provide anchorage and support
-site of energy (starch) storage
Definition
roots
Term
roots tip to plant
Definition
-root cap
-zone of cell division
-zone of elongation
-zone of maturation
Term
dicot roots
Definition
trap root system usually
Term
monocot root system
Definition
fibrous root system
Term
points at which leaves are attached
Definition
nodes
Term
stem segments between nodes
Definition
internodes
Term
the structure which has the potential to form a lateral shoot (branch)
Definition
axillary buds
Term
primary growth point (contains apical meristem)
-also known as terminal bud
-at top of stem
Definition
apical bud
Term
have a circular xylem and phloem formation
Definition
dicots
Term
have spread out xylem and phloem
Definition
monocots
Term
main photosynthetic organ
-generally consists of a flattened blade and a stalk (petiole)
-can also be modified
Definition
leaves
Term
-outer protective covering of plant
-consists of epidermis
-usually a single layer of tightly packed cells
Definition
dermal tissue system in leaves
Term
-waxy coating
-provides protective barrier against water loss
-can also hinder gas exchange
Definition
cuticle
Term
- openings (pores) on the underside of leaf
-allow gas exchange
-can be opened and closed by guard cells
Definition
stomata
Term
guard cell workings...
Definition
when turgid (full of water) they open
-when flaccid (lack water) they close
Term
monocot leaves
Definition
long/ stringy
Term
dicot leaves
Definition
rounded
Term
carries out long distance transport of materials between roots and shoots
Definition
vascular tissue system
Term
transports water and dissolved materials upwards from the roots to the shoots
Definition
xylem
Term
function in both water transport and support, part of xylem
Definition
tracheids
Term
parts of xylem
Definition
tracheids, vessel elements
Term
specialized for water transport, xylem part
Definition
vessel elements
Term
transports organic nutrients (sugars) downwards from leaves to roots or to developing leaves and fruits
Definition
phloem
Term
parts of phloem
Definition
sieve tube elements, campanion cells
Term
living elongated cells arranged end to end for conduction of sugar in phloem
Definition
sieve-tube elements
Term
move sugars and amino acids into and out of the sieve elements in phloem
Definition
campanion cells
Term
the central core of tissue in the stem or root of a vascular plant , consisting of xylem and phloem together with supporting tissues
Definition
stele
Term
these structures: roots, stem, and leaves are where what differ from pteridophytes, gymnosperms and angiosperms
Definition
bryophytes
Term
-small in stature because they lack lignified vascular tissue
-no true leaves or roots
-live primarily in moist habitats
Definition
bryophytes
Term
the loss of water vapor from leaves
Definition
transpiration
Term
as water molecules evaporate through the plant’s stomata
Definition
more water molecules are pulled upward
Term
water molecules are attracted to other water molecules
Definition
cohesion
Term
water molecules are attracted to other materials
Definition
adhesion
Term
-several cutting planes produce roughly identical pieces
-have a dorsal (top) and ventral (bottom) surface
-no front, back, left, or right sides
Definition
radial symmetry
Term
-only have one plane (sagittal plane) that will divide the organism into roughly mirror image halves
-cephalization appears
Definition
bilateral symmetry
Term
-first found in Eumetozoa
-sponges are animals with no tissues and no symmetry
Definition
animals can be defined by presence or absence of tissues
Term
ectoderm and endoderm
Definition
diploblastic
Term
ectoderm (epidermis of skin), mesoderm (notochord), endoderm (liver)
Definition
triploblastic
Term
-anus develops from blastopore first
-fate of cells is not determined early on
-second opening developed is mouth
Definition
radial cleavage -> deuterostomes
Term
-mouth develops from blastopore first
Definition
spiral cleavage -> protostomes
Term
-budding in hydra
-parthenogenesis in whiptail lizards
Definition
examples of asexual reproduction
Term
-sex that produces sperm
-can be heterogametic (XY), homogametic (ZZ in birds) or haploid in bees
Definition
males
Term
-sex that produces eggs
-can homogametic (XX), heterogametic (ZW in birds) or diploid in bees
Definition
females
Term
embryonic development
Definition
-zygote

-2/4/8 cell stages

-blastula

-gastrula
-early organogenesis

-late organogenesis
Term
2/4/8 cell stages
Definition
animal pole on top, vegetal pole on bottom
Term
blastula
Definition
blastocoel
Term
organogenesis
Definition
development of neural tube
Term
homeotic genes that control the form of anterior and posterior structures of the body occur in the same linear sequence on chromosomes in both species
Definition
colinearity
Term
anterior, lizard
Definition
nose
Term
dorsal, lizard
Definition
back
Term
ventral, lizard
Definition
bottom
Term
posterior, lizard
Definition
tail
Term
anterior, human
Definition
front
Term
posterior, human
Definition
back
Term
medial
Definition
closer to midline
Term
sagittal plane
Definition
length of body, up and down
-dorsal and ventral portions
Term
frontal plane
Definition
length of body, side to side
-left and right portions
Term
transverse plane
Definition
up and down, through middle
-cranial and caudal portions
-“cross section”
Term
digestive system in animals with bilateral symmetry
Definition
there is a gut tube that runs from the mouth to the anus
Term
digestive tract in animals with radial symmetry
Definition
there is only one opening into which food goes in and waste comes out
-this chamber is known as the gastrovascular cavity
Term
three major functions of circulatory system
Definition
-gas exchange
-nutrient distribution
-waste removal
Term
-oxygen enters through small openings (spiracles) along the abdomen
-the heart circulates the hemolymph, but not through a system of closed vessels
Definition
open circ. system
Term
the heart circulates blood through a closed system of vessels
Definition
closed circ. system
Term
how oxygen enters closed circ. system
Definition
-through the skin
-through gills
-through lungs
Term
arthropod characteristics
Definition
-bilateral symmetry
-segmented body
-hard exoskeleton
-jointed legs
-many pairs of limbs
Term
types of arthropods
Definition
crustacea, hexapoda, myriapoda, trilobites, cheliceriforms
Term
major body sections of hexapods
Definition
head, thorax, abdomen
Term
two parts of mammalian circulatory system
Definition
pulmonary system and systemic system
Term
pulmonary system
Definition
-heart to lungs, lungs to heart
-picks up oxygen from the lungs and gets rid of carbon dioxide
Term
-heart to body, body to heart
-delivers oxygen to tissues and picks up waste carbon dioxide
Definition
systemic system
Term
-collects blood that is delivered from lungs
-on right of diagram
-pulmonary veins bring blood from lungs
Definition
left atrium
Term
how many chambers in a mammalian heart?
Definition
4
Term
-on right, bigger/ lower part
-pumps blood to body through aorta
-very muscular so as to generate enough pressure to pump blood through the system circuit
Definition
left ventricle
Term
right atrium
Definition
-on left, top
-collects blood from body from vena cava
Term
right ventricle
Definition
on left, bottom
-pumps blood to lungs through pulmonary artery
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