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Biology 101 - Chapter 04
Biology 101 - Chapter 04 Tour of the Cell
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Biology
Undergraduate 1
10/27/2013

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Term
Protein
Definition
Macromolecule
Polymer of amino acid monomers.
Most elaborate and diverse of life's molecules.
Account for more than 50% of dry weight of most cells
Term
Amino acid
Definition
consists of a central carbon atom bonded to four covalent partners
Three bonds are common to all 20 amino acids:
Carboxyl group (-COOH)
Amino group (-NH2)
Hydrogen atom
Fourth bond of the central carbon is the R group (radical group)
Term
R group
Definition
radical group attached to an amino acid that gives it special chemical properties
Term
Peptide bond
Definition
Bond between amino acids resulting in a long chain of amino acids
Term
polypeptide
Definition
long chain of amino acids consisting of peptide bonds
Term
protein
Definition
polymer consisting of one or more polypeptides
Term
primary protein structure
Definition
amino acid sequence "the spelling" of a polypeptide

polypeptide chain
causes the protein to its functional shape
Term
protein shape
Definition
primary structure
secondary structure
tertiary structure
quaternary structure
Term
secondary protein structure
Definition
ONE polypeptide chain that forms:
alpha helix
pleated sheet
re-inforced by hydrogen bonds
Term
tertiary protein structure
Definition
ONE polypeptide chain
the next level of the three dimensional shape - polypeptide chain, alpha helix and pleated sheet together
Term
quaternary protein structure
Definition
proteins with two or more polypeptide chains
Term
denaturation
Definition
protein's shape is sensitive to the surrounding environment, unfavorabile temperature change, pH or other quality of the environment can cause a protein to unravel and lose its normal shape.
Term
Nucleic acids
Definition
macromolecules that store information and provide the instructions for building proteins
polymers of nucleotides
Term
DNA
Definition
deoxyribonucleic acid - genetic material that humans and other organisms inherit from their parents
Term
RNA
Definition
ribonucleic acid
Term
gene
Definition
specific stretch of DNA that programs the amino acid sequence of of a polypeptide
Term
nucleotides
Definition
monomer of nucleic acids
Term
DNA nucleotide
Definition
Center is a five carbon sugar,deoxyribose

attached to the sugar is negatively charged phosphate group (PO4-)

nitrogen containing base:
Adenine (A)
Guanine (G)
Thymine (T)
cytosine (C)
Term
RNA nucleotide
Definition
Center is a five carbon sugar, ribose

attached to the sugar is negatively charged phosphate group (PO4-)


nitrogen containing base:
Adenine (A)
Guanine (G)
Cytosine (C)
Uracil (U)
Term
sugar-phosphate backbone
Definition
repeating pattern of sugar-phosphate-sugar-phosphate with the bases (A, T, C, or G) hanging off the backbone like appendages
Term
Nucleotides that link
Definition
C & G
T & A
Term
double helix
Definition
double stranded molecule of cellular DNA wrapped around each other
Term
microscopes
Definition
light microscope (LM)
electronmicroscope(EM)
scanning electron microscope (SEM)
transmission electron microscope (TEM)
Term
light microscope (LM)
Definition
used by Renaissance scientists and same kind of microscope found in most biology classrooms
Uses visible light projected through a live specimen
Term
two major categories of cells
Definition
prokaryotic
eukaryotic
Term
prokaryotic cells
Definition
found in organisms of the domains Bacteria and Archaea

known as prokaryotes

contains a nucleoid, not a nucleus
Term
eukaryotic cells
Definition
found in organisms of the domain Eukarya - protists, plants, fungi and animals

known as eukaryotes
Term
all cells:
Definition
plasma membrane
cytosol
chromosomes
ribosomes
Term
plasma membrane
Definition
barrier around a cell
Term
cytosol
Definition
thick, jellylike fluid inside the cell
Term
chromosomes
Definition
carry genes made of DNA
Term
ribosomes
Definition
build proteins according to instructions from the genes
Term
prokaryotic cell
Definition
first life on earth, 3.5 billion years ago

much smaller - 1/10th length of eukaryotic cell

simpler in structure - no nucleus - nucleoid area
Term
eukaryotic cell
Definition
2.1 billion years ago

bigger and more complex in structure
Term
organelles
Definition
only in eukaryotic cells

membrane-enclosed structures that perform specific functions
Term
nucleoid
Definition
area of prokaryotic cell where DNA is coiled into a "nucleus like" region, not partitioned from the rest of the cell
Term
cytoplasm
Definition
prokaryotic cell: interior

eukaryotic cell: entire region of theA cell between the nucleus and the plasma membrane
Term
Animal cell
Definition
CENTRIOLE
LYSOSOME
ribosomes
cytoskeleton
plasma membrane
mitochondrion
rough endoplasmic reticulum (ER)
smooth endoplasmic reticulum (ER)
golgi apparatus
nucleus
Term
Plant cell
Definition
CENRAL VACUOLE
CELL WALL
CHLOROPLAST
plasma membrane
cytoskeleton
mitochondrion
nucleus
rough endoplasmic reticulum (ER)
smooth endoplasmic reticulum (ER)
ribosomes
golgi apparatus
Term
plasma membrane structure
Definition
composed mostly of phospholipids and proteins

one phosphate - two fatty acid tails
phosphate head is hydrophilic - tails are hydrophobic, creating a chemical ambivalence in their interactions with water
Term
phospholipid
Definition
related to dietary fats but only have two fatty acid tails instead of three

electrically charged, making it hydrophilic, but the two fatty acid tails are hydrophobic.

chemical ambivalence in their interactions with water- phosphate head mixes with water, but the tail avoids it
Term
phospholipid bilayer
Definition
two-layered membrane

hydrophobic fatty acid tails of the molecules stay in the membrane interior away from water, while the hydrophilic phospholipid heads remain surrounded by water inside and outside the cell

embedded in the bilayer of most membranes are proteins that help regulate traffic
Term
fluid mosaic membranes
Definition
fluid because the molecules can move freely past one another and a mosaic because of the diversity of proteins that float like icebers in the phospholipid sea
Term
cell surfaces
Definition
animal cells - secrete a sticky coat called the extracellular matrix

holds cells together in tissues
Term
cell junctions
Definition
animal cells - connects cells together into tissues
Term
nucleus
Definition
separated by a double membrane

contains DNA molecules and associated proteins form fibers called chromatin
Term
nuclear envelope
Definition
double membrane that separates the nucleus from the cytoplasm

pores in the nuclear envelope allow certain materials to pass through
Term
chromatin
Definition
each fiber constitutes one chromosome (humans contain 46 chromosomes, rice 24, dogs 78)
Term
nucleolus
Definition
prominent structure within the nucleus, where ribosomes are made
Term
ribosomes
Definition
responsible for protein synthesis

components are made in the nucleus and transported through the pores into the cytoplasm

ribosmes attach to the oustide of the nucleus or the endoplasmic reticulum
Term
endoplasmic reticulum
Definition
makes proteins that are incorporated into membranes and secreted by the cell
Term
DNA directing protein
Definition
1. DNA programs protein production into the cytoplasm by transferring its coded information to a molecule called messenger RNA

2. mRNA exits through pores in the nuclear envelope and travels to the cytoplasm where it binds to ribosomes

3.the ribosome moves along the mRNA, translating the genetic message into a protein with a specific amino acid sequence
Term
endomembrane system
Definition
nuclear envelope, endoplasmic reticulum, golgi apparatus, lysosomes and vacuoles

partions the cytoplasm of a eukaryotic cell
Term
endoplasmic reticulum
Definition
one of the main manufacturing facilities within the cell

connected to the nuclear envelope, forms an extensive labyrinth of tubes and sacs running throughout the cytoplasm

two components: rough and smooth - physically connected but differ in structure and function
Term
rough endoplasmic reticulum
Definition
ribosomes that stud the outside of its membrane

one function is to make more membrane, phospholipids are made by enzymes of the rough ER and are inserted into the membrane, growing the ER membrane

Portions of this membrane are transferred to other parts of the cell

salivary glands contain lots of rough ER
Term
transport vesicles
Definition
sacs made of membrane that bud off from the rough ER
Term
smooth endoplasmic reticulum
Definition
lacks the ribosomes on the membrane

responsible for synthesis of lipids, including steroids

in liver cells = enzymes of the smooth ER detoxify circulating sedatives such as barbiturates, stimulants and some antibiotics
Term
golgi apparatus
Definition
organelle that receives, refines, stores and distributes chemical products of the cell

receives products from the ER in a transport vesicle

consists of a stack of membrane plates
1) one side of the stack serves as a receiving dock for vesicles from the ER
2) proteins are usually modified by enzymes during their transit from the receiving to the shipping side of the golgi
3) shipping side of a stack is a depot from which finished products can be carried in transport vesicles to other organelles or to the plasma membrane
Term
Lysosome
Definition
membrane enclosed sca of digestive enzymes found in animal cells

absent from most plant cells

develop vesicles that bud off from the golgi apparatus

enzymes within a lysosome can break down large molecules such as proteins, polysaccharides, fats and nucleic acids

provides a compartment where the cell can digest these molecules safely without unleashing the digestive enzymes onto the cell itself
Term
Vacuoles
Definition
large sacs of membrane that bud from the ER, Golgi apparatus or plasma membrane
Term
central vacuole
Definition
more than half the volume of a mature plant cell

versatile compartment, stores organic nutrients (ex: proteins stockpiled in the vacuoles of seed cells)

also contributes to plant growth by absorbing water and causing cells to expand

in flower petal cells, may contain pigments that attract pollinating insects

may also contain poisons that protect against plant-eating animals
Term
chloroplasts
Definition
PLANT CELLS
photosynthetic cells of plants and algae, performs photosynthesis

contains own DNA that encodes some of their own proteins

it is believed to have evolved from free-living prokaryotes in the distant past
Term
chloroplast partitions
Definition
1) space between membranes
2) stroma - thick fluid within chloroplast
3) grana - network of membrane-enclosed disks and tubes
Term
grana
Definition
chloroplasts solar power packs, trap light and convert it to chemical energy
Term
mitochondrion
Definition
organelle of cellular respiration in nearly all cells

harvests energy from sugars and uses it to produce another form of chemical energy called ATP (adenosine triphosphate)

contains own DNA that encodes some of their own proteins

it is believed to have evolved from free-living prokaryotes in the distant past
Term
ATP
Definition
direct energy source for most cell work
Term
matrix
Definition
thick fluid inside the mitochondrion
Term
cristae
Definition
numerous infoldings inside the inner membrane of mitochonrion envelope

many enzymes and other molecules that function in cellular respiration are embedded in the inner membrane

maximizs ATP output
Term
cytoskeleton
Definition
infrastructure of the cell

network of fibers extending throughout the cytoplasm

serves as both skeleton and "muscles" for the cell, functioning in support and movement

nucleus is held in place by sytoskeletal cage of filaments, other organelles move along tracks made from microtubules
(ex: lisosome reaches food vacuole by taking many small "baby steps" along a microtubule track)
Term
microtubule
Definition
straight, hollow tubes composed of proteins contained in the cytoskeleton

guide the movement of chromosomes when cells divide
Term
dynamics of cytoskeleton
Definition
quickly dismantles in one part of the cell by removing protein subunits and re-form in a new location by reattaching the subunits

can change location, shape or even cause the whole cell or some par of its parts to move

contributes to amoeboid (crawling) movements of the protist Amoeba and some of our white blood cells
Term
cilia
Definition
shorter and more numerous than flagella and promote movement by a coordinated back and forth motion

some animal cells
almost never found on plant cells
Term
flagella
Definition
propel cells through their undulating, whiplike motions

often occur singly, such as sperm cells, but may also appear in groups on the outer surface of protists

some animal cells
almost never found on plant cells
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