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1. cross of dominant phenotype with unknown genotype (either AA or Aa) with recessive. 2. Look at babies to figure out unknown parental genotype; recessive babies indicate Aa rather than AA. |
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1. eukaryotes ONLY 2. 5' cap, 3' polyA tail, splcing, NTP modification |
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1. phosphate group 2. pentose sugar 3. (nitrogenous) base |
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strong covalent bond between adjacent nucleotides (within each strand) |
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weak bond between base pairs (velcro's 2 stands together) |
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1. double helix 2. anti-parallel stands strands composed of nucleotides phosphodiester-bonded together 4. 2 strands velcro'd together by H bonds |
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DNA replication - definition |
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DNA synthesis during S phase of Interphase (before mitosis or meiosis) |
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DNA replication - type of process |
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1. semi-cpnservative 2. bi-directional 3. semi-discontinuous |
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results in daughter DNAs that are half old and half new |
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1. leading starnd produces continuously (in one piece off 1 primer) 2. lagging strand produced in multiple Okazaki fragments off multiple primers |
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showed semi-conservative DNA replication in Neurospora Crassa |
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1. Showed "transforming principle" in Streptococcus pneumonia 2. heat-killed S + live R kills mice 3. transforming principle probably not (heat-killed/denatured) protein |
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1. sugar in DNA 2. lacks O on #2 carbon 3. symbolized by d in dNTP |
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1. DNA wound around histone proteins (bead) 2. eukaryotes only |
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Where DNA replication starts |
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chromosome material (histones + DNA) |
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covalently-bonded fragments that become the lagging strand |
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1. shared pair of electrons 2. strong bond 3. inside molecules |
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1. weak bond 2. e.g. between nitrogenous bases 3. e.g. inside proteins (folding) |
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covalent bond between sugar of first nucleotide and phosphate of second nucleotide |
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1. e.g DNA or RNA strands have a 5' beginning and a 3' end 2. e.g polypeptides have a N-terminus (amino) beginning and a C-terminal (carboxyl) end |
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1. spliced out of mRNA 2. not expressed into protein |
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pre-mRNA or primary transcript |
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1. 5' capping 2. 3' polyA tailing 3. Splicing $. RNA editing
(Eukaryotes ONLY!!!) |
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1. ready for translation 2. no introns 3. has 5' cap and 3' tail |
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1. mRNA into polypeptide 2. by ribosomes 3. in cytosol |
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1. amino acids peptide-bonded together 2. folded up |
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bi-directional DNA Replication |
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2 opposing forks moving away from OriC |
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semi-conservative DNA Replication |
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daughter DNA has 1 parental + new strand |
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2 strands running in opposite direction |
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semi-discontinuous DNA Replication |
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continous on leading strand; discontinuous on lagging strand |
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