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Biochemistry- Unit One
Biological Buffers (T Pierce)
10
Medical
Post-Graduate
01/05/2009

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Cards

Term

pH of blood

Definition
7.4
Term
Major intracellular buffer systems in body
Definition

phosphate

protein

Term
Major extracellular buffer system of body
Definition
bicarbonate
Term
What factors is hydrogen concentration determined by within a buffer system?
Definition
  • potential for the acid to become an acid (Ka)
  • ratio of acid to the base (actual concentration of components in solution)
Term
Hydrogen concentration in blood
Definition
40 nanomoles/L (nM) = 7.4 pH
Term
What must the ratio of bicarbonate to carbonic acid in the blood be for the pH to remain 7.4?
Definition
20 to 1
Term
Total concentration of all components of bicarbonate buffer system.
Definition
  • 25.2 mmoles/L (mM)
    • bicarbonate = 24 mM (20/21 X 25.2 mM)
    • carbonic acid/carbon dioxide = 1.2 mM (1/21 X 25.2 mM)
Term
Function of kidneys in bicarbonate buffer system
Definition
  • regenerate HCO3 and release it into blood
  • excrete hydrogren ion
Term
Forms of phosphate that are predominate in the blood. Why?
Definition
  • dihydrogen phosphate and hydrogen phosphate
  • the pKa of these two compounds is about 7, and the pH of the blood is 7.4
Term
How does the phosphate buffer system help in conserving sodium in blood?
Definition

Normally, different forms of phosphate have a certain number of hydrogen ions associate with them (ex: dihydrogen phosphate has one sodium). When they enter the kidney, they no longer need the sodium because they can combine with the hydrogen ions in the kidney filtrate. Remember, the kidney has the tendency to excrete hydrogen. By adding this hydrogen, I have just lowered the amount of cations needed to balance the reaction between dihydrogen phosphate and hydrogen phosphate by driving the reaction in favor of dihydrogen phosphate.

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