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Biochemistry
RUSM Semester 1
42
Biochemistry
Graduate
05/08/2012

Additional Biochemistry Flashcards

 


 

Cards

Term
Define Endergonic and give an example of an endergonic reaction
Definition
Requires energy - peptide linkage
Term
Name this functional group: -COOH
Definition
Carboxyl group
Term
Name this functional group: -NH2
Definition
Amino group
Term
Name this functional group: -CH3
Definition
Methyl group
Term
Name this functional group: -COH
Definition
Carbonyl (aldehyde group)
Term
Name this functional group: R-CO-R'
Definition
Carbonyl (ketone)
Term
Name this functional group: R-COO-R'
Definition
Ester group
Term
Name this functional group: R-CO-R'
Definition
Ether group
Term

[image]

Name the linkage type for 1 and 2 in this glucose bonding.

Definition

1. Axial (alpha)

2. Equitorial (beta)

Term
Define what it means when a molecule is chiral
Definition

When it is not superimposable on its mirror image.

 

Easy way to identify is if the central carbon has 4 different types of molecules.

Term
Define a achiral molecule
Definition
A molecule that can be roated and be superimposed on its mirror image
Term
Define what an enantiomer is
Definition
Stereoismores whose molecules are NON-superimposable mirror images of one another
Term
True or False: Enantiomers have share all physical properties of their mirror images aside from light rotation.
Definition
True
Term
A molecule that rotates light clockwise is...
Definition
(+)-dextrorotary
Term
A molecule that rotates light anti-clockwise is...
Definition
(-)-levorotatory
Term
An equimolar mixture of two enantiomers (or a racemic mixture) has what degree of rotation?
Definition
0
Term
What is the importance of enantiomers in biological processes?
Definition
An L-enantiomer could have function in the body where the R-enantiomer could have no function or a destructive function in the body, or vice versa.
Term
What would classify a steroisomer as a diastereomer?
Definition
Stereoisomers that are not mirror images of each other. Molecules with 2 or more chiral centers
Term
Which enantiomer is active in the body normally.
Definition
The L-enantiomer.
Term
What are compounds with the same chemical formula called?
Definition
isomers
Term
How do constitutional isomers differ from eachother?
Definition
different physical properites, different chemical properties, different IUPAC names, different (sometimes the same) functional groups
Term
How do sterioisomers differ from eachother?
Definition
Only differ by the way their atoms are oriented in space.
Term
How can you determine the number of stereoisomers of a molecule?
Definition
2^(# of asymmetric carbons)
Term
A silver mirror of the Tollens reagent or a brick red precipitate of the Benedict's reagent would indicate you have what type of sugar?
Definition
A reducing sugar (-OH exposed)
Term
Name the makeup and linkage of Maltose.
Definition
Glucose, Glucose; a(1-4)
Term
Name the makeup and linkage of Lactose.
Definition
Galactose, Glucose; B(1-4)
Term
Name the makeup and linkage of Cellobiose.
Definition
Glucose, Glucose; B(1-4)
Term
Name the makeup and linkage of Sucrose.
Definition
Fructose, Glucose; aB(1-2)
Term
Which form of starch has branches and which does not?
Definition
Amylose does not have branches, amylopectin does.
Term
Name the main difference between Glycogen and Starch.
Definition
Glycogen is 'energy-ready' with more branch points that can be hydrolyzed.
Term
The antiparallel nature of double-stranded DNA means that:

1. The two strands run from 5’ to 3’ in opposite directions.
2. The 5’ ends of the two strands are on the opposite ends of the DNA molecule.
3. The two DNA strands twist around each other in a helical pattern.
4. 1 and 2.
5. 1 and 3.
Definition
4. 1 and 2.
Term
While studying the structure of a small gene that was recently sequenced during the Human Genome Project, an investigator notices that one strand of the DNA molecule contains 20 A’s, 25 G’s, 30 C’s, and 22 T’s. How many of each base is found in the complete double-stranded molecule?

1. A=40, G=50, C=60, T= 44
2. A=44, G=60, C=50, T= 40
3. A=45, G=45, C=52, T= 52
4. A=50, G=47, C=50, T= 47
5. A=42, G=55, C=55, T= 42
Definition
Term

If a completely radioactive double-stranded DNA molecule undergoes two rounds of replication in a solution free of radioactive label, what is the radioactivity status of the resulting four double-stranded DNA molecules?

1.Half should contain no radioactivity
2.All should contain radioactivity
3.Half should contain radioactivity in both strands
4.One should contain radioactivity in both strands
5.None should contain radioactivity
Definition
1.Half should contain no radioactivity
Term

Which of the following is the initiator protein(s) that must bind to DNA before any other proteins needed for replication can bind?

1.Cdc6p
2.MCM
3.DNA polymerase delta
4.ORC
5.CDK
Definition
4.ORC
Term

What modification does CDK and DDK do to the proteins, associated with replication initiation, that inhibits the use of an origin of replication more than once during the cell cycle?

1.Acetylation
2.Phosphorylation
3.Methylation
4.Ubiquitination

5.Glycosylation 

Definition
2.Phosphorylation
Term

During DNA replication, which type of enzyme separates double-stranded DNA into the single-stranded DNA that is needed as a template for the DNA polymerases?

1.DNase
2.Topoisomerase
3.Helicase
4.Primase
5.Single-strand DNA binding (SSB) protein
Definition
3.Helicase
Term

Which type of enzyme is responsible for preventing 'overwinding' of DNA?

1.DNase
2.Topoisomerase
3.Helicase
4.Primase
5.Single-strand DNA binding (SSB) protein
Definition
2.Topoisomerase
Term

Inhibitors of DNA replication are most likely to find medical use as:

1.Anti-fungal agents
2.Treatments for immunodeficiency diseases
3.Antibiotics
4.Anti-cancer drugs
5.Treatments for psychiatric conditions such as schizophrenia
Definition
4.Anti-cancer drugs
Term

What is the polymerase that generates the primer in eukaryotes called?

1.DNA polymerase δ
2.DNA polymerase ε
3.DNA polymerase β
4.DNA polymerase γ
5.DNA polymerase α
Definition
5.DNA polymerase α
Term
Which polymerase is assigned to the leading strand?
Definition

Pol ε

 

Term
Which polymerase is assigned to the lagging strand?
Definition
Pol δ
Term

Which of the following keeps the DNA polymerase on the DNA?

1.PCNA
2.γ-clamp
3.MCM
4.Pol δ
5.RPA
Definition
1.PCNA
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