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Biochemistry
First Test Material
117
Biochemistry
Professional
10/08/2011

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Term
What are the three main tenets of the cell theory?
Definition
cell is the basic unit of life, all living organisms are composed of cells, cells come from pre-existing cells
Term
What are the four things that all cells contain?
Definition
plasma membrane, cytoplasm, ribosomes, and DNA
Term
Are ribosomes membrane bound organelles?
Definition
No
Term
What do prokaryotes lack compared to eukaryotes?
Definition
nucleus and membrane bound organelles
Term
Where does a phototroph get its energy from?
Definition
sunlight
Term
Where does an autotroph get its carbon from?
Definition
carbon dioxide
Term
Where does a heterotroph get its carbon from?
Definition
organic material
Term
What is meant by covalent?
Definition
sharing electrons
Term
What does a condensation reaction do?
Definition
attaches monomers by removing a molecule of water
Term
What does hydrolysis do?
Definition
breaks down molecules by adding water
Term
What is the bond that makes triacylglycerides?
Definition
ester bonds
Term
What law of thermodynamics says that you canot create or destroy energy?
Definition
first law
Term
What law of thermodynamics says that you can change an energy's form?
Definition
First law
Term
What law of thermodynamics says that the everything in the universe is moving towards disorder?
Definition
Second law of entropy
Term
What is the loss of electrons called?
Definition
oxidation
Term
What is the gain of electrons called?
Definition
Reduction
Term
How do enzymes speed up chemical reactions?
Definition
Lowering the activation energy
Term
An anabolic pathway does what?
Definition
building up, biosynthetic
Term
A catabolic pathway does what?
Definition
break things apart, degradative
Term
What kind of replication does DNA go through?
Definition
semi-conservative replication
Term
Would the replication of DNA be anabolic or catabolic?
Definition
Anabolic (building up)
Term
What is transcription?
Definition
DNA to mRNA
Term
Where does trascription take place?
Definition
nucleus in eukaryotic cells, cytoplams in prokaryotic cells
Term
What is osmosis?
Definition
diffusion of water following its concentration gradient through a semipermiable membrane
Term
Where does water move in a hypertonic cell?
Definition
out of the cell (more water inside the cell)
Term
Where does water move in a hypotonic cell?
Definition
into the cell (more water outside the cell)
Term
What is the average pH of urine?
Definition
6
Term
What is the average pH of blood?
Definition
7.3
Term
What is the average pH of cerebral spinal fluid (CSF)?
Definition
7.4
Term
What is the average stomach pH?
Definition
2 to 3
Term
What is the pH of saliva?
Definition
6.5
Term
What is the pH of intestinal fluids?
Definition
8.2
Term
What are the amino acids containing sulfur?
Definition
Methionine and cysteine
Term
What are the positively charged amino acids?
Definition
Histadine, Argainine, Lysine
Term
What are the negatively charged amino acids?
Definition
aspartic acid, and glutamic acid
Term
What are the non-polar amino acids?
Definition
Valine, Methionine, Leucine, Alanine, Glycine, Isoleucine, Proline
Term
What are the essential amino acids?
Definition
Phenolalanine, Valine, Threonine, Tryptophan, Isoleucine, Methionine, Histidine, Alanine, Leucine, Lysine
Term
What proteins have primary structure?
Definition
All proteins have primary structure
Term
What happens if you change the structure of a protein?
Definition
You change its function
Term
What does a protease do?
Definition
Break down proteins (pepsin, chymotrypsin, trypsin)
Term
Where does Pepsin break peptide bonds?
Definition
Aromatic (Phe, Trp, Tyr) on the AMINO side
Term
Where does chymotrypsin break peptide bonds?
Definition
Aromatic (phe, try, trp) on the carboxyl side
Term
Where does trypsin break peptide bonds?
Definition
Lysine, Arginine, and Histadine
Term
What increases blood glucose levels?
Definition
Glucagon
Term
What decreases blood glucose levels?
Definition
Insulin
Term
What is the function of carbonic anhydrase?
Definition
converts carbon dioxide and water to bicarbonate and the reverse reaction
Term
What does hemoglobin do?
Definition
carries or transports oxygen and carbon dioxide in the blood
Term
What does Lactate dehydrogenase enzyme do?
Definition
converts pyruvate to lactate; reduction reaction
Term
What does ceruloplasmin do?
Definition
transports copper
Term
What takes glycogen and makes glucose-1-phospate?
Definition
glycogen phosphorylase
Term
What does pyruvate dehydrogenase do?
Definition
pyruvate to acetyl CO-A; oxidation reaction
Term
What enzyme stores iron?
Definition
ferritin
Term
What enzyme transports iron?
Definition
transferrin
Term
What is an active site of an enzyme?
Definition
a specific site where a substrate can bind with the enzyme
Term
What is an allosteric site?
Definition
seperate than the active site where inorganic or organic bind to make the enzyme function
Term
What kind of activity does an oxidoreductase have?
Definition
electron transfer
Term
What type of activity does a lyase have?
Definition
breaking bonds
Term
What kind of activity does a hydrolase have?
Definition
using water to break bonds
Term
What kind of activity does a ligase have?
Definition
hooking things together
Term
What kind of activity does a transferase have?
Definition
group transfer between molecules (intermolecular arrangement)
Term
What kind of activity does an isomerase have?
Definition
rearrangement within a single molecule (intramolecular)
Term
What are the three types of control mechanisms that regulate enzyme activity?
Definition
pH, temperature, and inhibitors (competitive and noncompetitive)
Term
What is an inactive precursor called?
Definition
zymogen
Term
What is partial proteolysis?
Definition
removing a piece of the enzymes structure, usually to activate an enzyme; irreversible
Term
What does a phosphatase enzyme usually do?
Definition
adding phosphate
Term
What does a phosphatase enzyme do?
Definition
removes phosphates
Term
Feedback inhibition is also known as what?
Definition
allosteric control or non-competitive inhibition
Term
What is the functional name of vitamin B1?
Definition
Thiamin
Term
What is the active form of B1?
Definition
Thiamin pyrophosphate (TPP)
Term
What vitamin is needed for reactions that convert aldehydes into ketones, or vice versa?
Definition
B1, Thiamin, TPP
Term
What can a deficiency in vitamin B1 cause?
Definition
build up of pyruvate, compromised energy production, Beri Beri
Term
What is the functional name of vitamin B2?
Definition
Riboflavin
Term
What is the active form of vitamin B2?
Definition
FAD
Term
What does a deficiency in riboflavin cause?
Definition
General decreased energy
Term
What is the functional name of vitamin B3?
Definition
Niacin
Term
What is the active form of vitamin B3?
Definition
NAD and NADP
Term
Deficiency of vitamin B3 causes what?
Definition
Pellagra
Term
What are the symptoms of pellagra?
Definition
dermatitis, diarrhea, dementia, death
Term
What is the functional name of vitamin B5?
Definition
Panthothenic acid
Term
What is the active form of Pantothenic acid?
Definition
CO-enzyme A
Term
What is the functional name of B6?
Definition
Pyridoxine
Term
What is the active form of B6?
Definition
Pyridoxal phosphate
Term
What are the functions of pyridoxine (B6)?
Definition
protein metabolism and transaminations
Term
What does a deficiency of B6 cause?
Definition
a build up of amino groups or ammonia
Term
What is biotin's active form?
Definition
biocytin
Term
What is the function of biotin?
Definition
carbon carrier, pyruvate carboxylase, proprionyl CO-A carboxylase
Term
What happens if you have a deficiency of biotin?
Definition
decrease in metabolism
Term
What is the functional name of B9?
Definition
Folate
Term
What is the active form of B9?
Definition
tetrahydrofolate
Term
What is the function of folate (B9)?
Definition
metabolism of amino acids and nucleic acids
Term
What does deficiency of vitamin B9 cause?
Definition
inhibition of DNA synthesis leads to megaloblastic anemia; neural tube defects; increased risk of cancer
Term
What is vitamin B12's functional name?
Definition
cobalamin
Term
What is the active form of cobalamin (B12)?
Definition
cobalamin
Term
What are the functions of vitamin B12?
Definition
extrinsic factor to avoid pernicious anemia
Term
What vitamin contains cobalt?
Definition
Vitamin B12 (cobalamin)
Term
What does a deficiency of B12 cause?
Definition
increased demyelination b/c decreased fatty acid synthesis; pernicious anemia
Term
What is the functional name of vitamin C?
Definition
ascorbic acid
Term
What is the active form of ascorbic acid?
Definition
ascorbic acid
Term
What is the function of vitamin C?
Definition
antioxidant; collagen production
Term
What does a deficiency of vitamin C cause?
Definition
sagging skin, facial lines; poor collagen; Scurvy
Term
What can lower levels of Vitamin C?
Definition
smoking and stress
Term
What are the fat soluble vitamins?
Definition
ADEK
Term
What is the functional name of vitamin A?
Definition
Beta carotene
Term
What is the active form of vitamin A?
Definition
retinol
Term
What are the functions of vitamin A?
Definition
antioxidant; healthy skin, bones and teeth; needed for production of night vision pigments
Term
What does a deficiency of vitamin A cause?
Definition
poor skin quality, bone, and teeth; night blindness
Term
What is the functional name of vitamin D?
Definition
cholecalciferol
Term
What is the active form of vitamin D?
Definition
1,25-dihydroxycholecalciferol
Term
What is the function of vitamin D?
Definition
calcium metabolism
Term
What does a deficiency of vitamin D cause?
Definition
poor quality bones, rickets, and osteomalacia
Term
What is the functional name of Vitamin E?
Definition
Tocopherol
Term
What is the active form of vitamin E?
Definition
tocopherol succinate
Term
What is the functions of vitamin E?
Definition
antioxidant, stabilize cell membrane, prevents epoxides from forming (rancid lipids)
Term
What does a deficiency of vitamin E cause?
Definition
membrane instability
Term
What is the active form of vitamin K?
Definition
phylloquinone or phytylmenaquinone
Term
What is the function of vitamin K?
Definition
blood coagulation components
Term
What happens if their is a deficiency of vitamin K?
Definition
clotting problems
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