Term
| what are the subunits and their size for prok |
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Definition
5s large
16s small
23s large |
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Term
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Definition
5s Large
5.8s large
18s small
28s large |
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Term
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Definition
Prokaryotic
composed of 50s (23s and 5s + proteins)
and 30s (16s + proteins) |
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Term
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Definition
Eukaryotic
Composed of 60s (28s+5.8S+proteins) large subinits
and a 40S (18s) Small |
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Term
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Definition
23s + 5s and proteins
prokarytoic |
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Term
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Definition
16s and proteins
prokaryotic |
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Term
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Definition
28s+ 5+ 5.8 and proteins all large
eukaryotic |
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Term
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Definition
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Term
|
Definition
most effective against RNA pol II
transcribes mRNA and snRNA |
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Term
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Definition
| transcribe the 18, 5.8, and 28s subunits of rRNA |
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Term
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Definition
transcribe mRNA, and snRNA
sensitive to a amanitin
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Term
|
Definition
rRNA 5s
snRNA
tRNA
structural RNA <400bp |
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Term
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Definition
1 binds B subinit of which inhibits RNA pol
2 it is liophilic and can cross the BB.
3 used to treat meninigitis, tuberculosis, leprosy and MRSA
4 activates cytochrom p 450 in liver --> inc secretion and clearnace from body which hinders effectiveness of other drugs
5 poisonous to liver, increases its functioning |
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Term
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Definition
castor oil poison
removes single base adenine from 28s rRNA
this inactivates ribosomes large subunit (60S)
adenine = crucial for interxn with ef1 and 2
inactivation of ribosome leads to cell death |
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Term
| descrive how rRNA is tx from DNA |
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Definition
from acrocentric chromosome 13,14,15,21,22
as one large transcript which is cut up by RNASE |
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Term
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Definition
have unusual intrachain base pairing
and unusual bases.
only rna to have thymine
3' end has the aa attached to adenosine |
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Term
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Definition
Maternally Inherited diabetes and Deafness
tRNA mutatuion for leucin
3423 mutation
a-->G for lysine
t--> fir leucine
mitochondrial np 3243 mutation of the tRNA Leu |
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Term
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Definition
mitochondrial encephelpathy Lactic acidosis
Trp-->Leu mutation
Stroke like episodes
cortical blindnes, focal seizure, encephalopathy, diabetes |
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Term
| mutation in tRNA leu in mitochondria |
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Definition
causes complex 1 deficiency
g mutation
affects atp production
complex has high [leu] residues |
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Term
| LAC operon characteristics |
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Definition
Z gene- cleaves lactose into galac and gluc
y= permease
a= transacetylase
operator binds repressor, it induces it
repressor binds at the promoter |
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Term
| characterisitics of bacterial operon |
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Definition
conatins 2 genes. 1 promoter. 2 ribosomal binding sites.
2 aug starts. 2 AAu stops |
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Term
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Definition
5' region of prok mRNA. binds to PUrine rich
16s ribosomal (Small) |
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Term
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Definition
| RNA POL halo enzyme binds up stream of initiation site. known as pribnow or TATA box 8-10 nucleotide upstream of AUG. another consesus seq TTGACA is at -35. the closer these seqs are to given seq, the more likely gene is txed. |
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Term
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Definition
| helps RNA pol bind promoter region (-10 TATA box) tightly. aka intiation factor. incr affinity for promoter. has affinity for -35 seq |
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Term
| structure of Prok RNA pol |
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Definition
2 alpha subunit= binds -35
b= polymerizing sub unit. makes phospodiester bonds
b' binds dna template
sigma= -10 region
a2bb'= core
core + sigma = haloenzyme |
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Term
| Rho indep terminiaton= type 1 |
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Definition
Prokarytoes. poly AU stretch= region where polymerase binds weakly. has its own atp. It gollow the gc rich hairpin loop. destablizes dna rna hybrid
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Term
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Definition
| lack of poly u stretch on so poly reqs rho facotr |
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Term
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Definition
done by rna polII
reqs- 25-30 TATA
-70 CAT box |
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Term
| Enhancer for Silencer on DNA |
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Definition
in EUK
enhancer is upstream of start site. make pol move faster
Silencer is not orienation nor position speific. |
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Term
| pre-mRNA processing steps |
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Definition
1. addition of 5' cap
2. splicing out of introns
3. addition of poly a tail |
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Term
| region of RNA POL involved in mRNA mods |
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Definition
| always ctd. proteins attach here and like cap, splice, or polyadenylate. |
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