Term
| How many PCR cycles does it take before you get a product that is the right length? |
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Definition
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Term
| Why is taq polymerase (heat resistant polymerase used in pcr) prone to make mistakes? |
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Definition
| It lacks the 3'-5' exonuclease |
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Term
| Which primers will amplify my favorite gene if it is in the context below? 5'-GGTACAGTC-MY FAVORITE GENE-CTAGATCAT-3' |
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Definition
| 5'-GGTACAGTC and 5'-ATGATCTAG |
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Term
| DNA sequencing depends on generating a set of molecules that terminate at every possible position. This is done by stopping the synthesis by using what? |
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Definition
using modified nucleotides lacking the 3' hydroxyl group.
the 3’ hydroxyl is needed for elongation so removing it causes termination |
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Term
| When modifying the Southern blot procedure to do a Northern blot, you would want to avoid the step in which... |
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Definition
the gel is soaked in sodium hydroxide to denature the nucleic acids.
RNA is sensitive to hydrolysis in solutions with high pH values, so this would tend to destroy the RNA that is being tested in a Northern |
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Term
| A DNA molecule is labeled and used to locate complementary strands in a restriction enzyme digest of genomic DNA separated by electrophoresis. What is this called. |
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Definition
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Term
| You have probed a Southern blot with a labeled DNA probe but find that you get hybridization to too many "target" sequences. You correctly infer that you are getting hybridization to sequences similar to, but not identical to, the intended target. How would you increase the specificity of the hybridization? |
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Definition
| Increase the hybridization temperature, decrease salt concentration, increase organic solvent or anything else that destabilizes the duplex making it more likely that only correct duplex matches will remain |
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Term
| How many POTENTIAL translational reading frames does a double-stranded DNA molecule have? |
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Definition
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Term
| A virus with a single-stranded RNA genome makes its way into a human cell and is taken directly to the translation machinery. How many potential reading frames does this nucleic acid have? |
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Definition
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Term
All of the following methods can be used to diagnose an infection with HIV. Which will produce a reliable signal the soonest after infection?
A. A Southern blot
B. A Northern blot
C. A Western blot
D. PCR
E. A microarray |
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