Term
|
Definition
| a modification of a VIM sequence at the C terminus of proteins, such as K-Ras |
|
|
Term
|
Definition
| another name for plasma membrane |
|
|
Term
|
Definition
| End of a prenylated sequence used to aggregate at membrane of important signaling protein. Has +8 charge. |
|
|
Term
|
Definition
| Modified K-Ras with alanine substituted for Threonine and Serine. Prevents phosphorylation. |
|
|
Term
|
Definition
| Modified K-pre with arginine substituted for lysine. Prevents ubiquitination. |
|
|
Term
|
Definition
| A solvochromatic dye that is excited near the membrane. Used to track movement of probe from donor to acceptor liposome. |
|
|
Term
|
Definition
| Post-translational modification which forms amide bonds of glycine to membrane. |
|
|
Term
|
Definition
| phoshatidylinositol-4,5-biphosphate. -5 charge. Contains inositol. |
|
|
Term
|
Definition
| Phosphoserine. -2 charge. Oleic and palmatic acid chains. Is flipped by AnnexinV and is a marker of cell death if on outer leaflet. |
|
|
Term
|
Definition
| Phosphotidylethanolamide. Uncharged. |
|
|
Term
|
Definition
| Phosphocholine. Uncharged. Primary component of donor liposomes. |
|
|
Term
|
Definition
| Pleicstein Homology Domain. Confers proteins to bind to PIP2. |
|
|
Term
|
Definition
| Protein that associates with PIP2 at membrane (cleaves PIP2 to IP3 and DAG) |
|
|
Term
|
Definition
| Antibody for PS on outerleaflet. Used typically to detect cell death. |
|
|
Term
|
Definition
| Glycosylphosphatidylinositol. Targets rafts. Used to confirm membrane integrity. |
|
|
Term
|
Definition
| transmembrane chimeric domain. |
|
|
Term
|
Definition
| farnesylated and diacylated membrane binding |
|
|
Term
|
Definition
| Phosphatidic Acid. -2 charge |
|
|
Term
|
Definition
| Carrier of Ca2+ into cytosol. Increases charge and lipid scramblase activity, increasing PS on membrane. |
|
|
Term
|
Definition
| Red fluorescent protein - used as the fusion protein dye |
|
|
Term
|
Definition
| inhibits cytochrome c function in mitochondria, and blocks ATP production. No PIP2 is synthesized and probes leave membrane. |
|
|
Term
|
Definition
| Flips PS to outer leaflet. |
|
|
Term
|
Definition
| A glycolipid with ceramide (a sphingosine+fatty acid) and charge. Found in gel phase. Detected by CTB. |
|
|
Term
|
Definition
| Triton X-100. When applied to lipids, it flushes out dye in fluid phase. Unable to affect rafts. |
|
|
Term
|
Definition
| Texas Red - 1,2-dipalmitoyl-sn-glycero-3-phosphocholine |
|
|
Term
|
Definition
| localizes to rafts - makes rafts thicker |
|
|
Term
|
Definition
| what lipid rafts "float" on |
|
|
Term
|
Definition
| double bonded, saturated fatty acid chain |
|
|
Term
|
Definition
| A fluoroscein conjugated lipid with DPPE. Likely green. |
|
|
Term
|
Definition
| Usually saturated, with double bounds near head |
|
|
Term
|
Definition
| Double bonds in unsaturated lead to "kinks" and a lower TM (more liquid) |
|
|
Term
|
Definition
| Used to detect GM1 in lipid rafts |
|
|
Term
|
Definition
| Mostly saturated lipid found used to form lipid gel phases |
|
|
Term
|
Definition
| Palmitoyl-oleoyl-glycero-phosphocholine |
|
|
