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Biochem Exam 5
Biochem Exam 5
30
Biology
Graduate
04/27/2010

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Term
The nucleotide sequence of DNA is which level of structure?
Definition
primary
Term
What level of structure is not present in nucleic acids?
Definition
quaternary
Term
The double helix of DNA is which level of structure?
Definition
secondary
Term
What distinguishes nucleotides from nucleosides?
Definition
Nucleosides lack the phosphate group
Term
What is the most common modification to nucleic acid bases?
Definition
reduction of a carbonyl to an alcohol
Term
Phosphate is important in nucleic acids because it can form a phosphodiester bond? TRUE/FALSE
Definition
TRUE
Term
Which base is not found in RNA?
Definition
Thymine
Term
Which nucleobases are purines?
Definition
Adenine and Guanine
Term
The fundamental differences between RNA and DNA are:
Definition
bases and ribose units
Term
Which of the following groups are not found in nucleosides? phosphates; purines; pyrimidines; sugars
Definition
phosphates
Term
The backbone of nucleic acids consists of:
Definition
a phosphodiester bond between the 3' and 5' hydroxyl groups of neighboring sugars
Term
The oligonucleotide AGGUCCAUUGAAp, is best described as...
Definition
an RNA oligonucleotide
Term
Histones are proteins that...
Definition
are frequently associated with eukaryotic DNA
Term
What is the dominant form of DNA?
Definition
BDNA
Term
The Z-form of DNA...
Definition
tends to occur in alternating purine-pyrimidine sequences.
Term
Topisomerases are associated with...
Definition
supercoiling of DNA
Term
Which of the following RNAs is the least abundant in a typical cell?
Definition
mRNA
Term
tRNA bonds to amino acids in the course of protein synthesis. TRUE/FALSE
Definition
TRUE
Term
small nuclear RNA is...
Definition
found in snurps; is involved in removing introns from the RNA; first shown to have catalytic activity
Term
Duplication process of DNA is called...
Definition
replication
Term
Flow of genetic information is RNA>>>DNA in:
Definition
retroviruses
Term
Semiconservative replication implies that...
Definition
each of the new double stranded DNA molecules contains one of the original intact strands and one completely new strand.
Term
How do origins of replication differ in eukaryotes and prokaryotes?
Definition
eukaryotes have several origins, while prokaryotes typically have one
Term
The direction of synthesis of DNA is...
Definition
from the 5' to the 3' end on both strands
Term
An important process in the synthesis of new DNA is...
Definition
proofreading and repair; unwinding of the double helix; protection of single-stranded regions from nuclease action
Term
When the synthesis of new DNA is directed by an original template DNA molecule...
Definition
two DNA molecules are formed, each with one new strand and one strand from the original
Term
Which is NOT a function of DNA polymerase 3? polymerization; ligating the final products; proofreading; clamping on to the template
Definition
ligating the final products
Term
The following enzyme is responsible for the bulk of DNA synthesis during replication?
Definition
DNA Polymerase 3
Term
The universal features of DNA replication include:
Definition
release of PPi from a nucleoside triphosphate; synthesis from the 5' end to the 3' end; base pairing of a A to T and G to C; use of a primer
Term
What function does DNA polymerase 3 lack?
Definition
5' to 3' exonuclease activity
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