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Biochem block 3, wk 3- AA degradation and urea cycle
Med school biochem
24
Biochemistry
Graduate
11/01/2010

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Term
ammonia transport AA's
Definition
glutamine, alanine, and serine
Term
What does aminotransferase stick an ammonia on
Definition
alpha-ketogulturate from CAC
Term
What is required cofactor for aminotransferase
Definition
PLP (Vit B6 deriv)
Term
Remove ammonia from serine and threonine
Definition
dehydratases
Term
release free ammonia from AA's
Definition
lyases
Term
remove ammonia from purines and pyrimidines
Definition
deaminases
Term
What are the enzymes that do: alpha-ketogluturate--> glutamate--> glutamine
Definition
1) glutamate dehydrogenase 2) glutamine synthetase
Term
can use both NADPH and NADH
Definition
Glutamate Dehydrogenase
Term
what transfers ammonia from glutamate to pyruvate
Definition
alanine aminotransferase
Term
glutamine and glutamate synth in cytosol or mitoch?
Definition
cytosol
Term
carbamoyl phosphate synth in cytosol or mitoch?
Definition
mitoch
Term
ammonia released in cytosol or mitoch
Definition
mitoch- this is how it looks in figure, not sure if it is only placed released
Term
releases ammonia from glutamine
Definition
glutaminase
Term
releases ammonia from glutamate
Definition
glutamate DH
Term
converts ammonia and bicarb to carbomoyl phosphate (where does this occur. What are the two things it reqs
Definition
phosphate synthetase I (in mitoch). 2 ATP and N-acetylglutamate
Term
What is N-acetylglutamate synth from
Definition
acetyl CoA and glutamate
Term
Availability and lvls of what four things control urea cycle
Definition
arginine, glutamate, and acetyl CoA and lvls of N-acetylglutamate synthase
Term
what catalyzes formation of citruline from ornithine and carbamoyl phosphate
Definition
ornithine transcarbamoylase
Term
Catalyzes activation of citulline to the intermediate citrullyl-AMP
Definition
Argininosuccinate synthetase reaction
Term
Aspartate displaces AMP on citrullyl-AMP intermediate to form what molec
Definition
argininosuccinate
Term
Catalyzes cleavage of argininosuccinate to fumurate and arginine
Definition
Argininosuccinate lyase
Term
Catalyzes cleavage of arginine into ornithine and urea
Definition
arginase
Term
how does benzoate work as a nitrogen waster?
Definition
picks up an N from glycine
Term
how does phenylacetate work as a nitrogen waster?
Definition
picks up TWO N's from glutamine
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