Term
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Definition
| Fractionation of protein through gel matrix |
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Term
| How does protein in AF seperate? |
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Definition
| By gravity, pressure or capillary action. No electricity used |
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Term
| What is a limitation of AF? |
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Definition
| Lack of specificity for proteins |
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Term
| Substance: Antibody seperated what is it's ligand? |
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Definition
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Term
| Polysaccharide Seperated what is it's ligand? |
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Definition
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Term
| What is bounded to the bead? |
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Definition
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Term
| Wat is passed through the column? |
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Definition
| impure mixture containing the protein to be isolated |
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Term
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Definition
| albumin binds to this dye |
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Term
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Definition
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Term
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Definition
| protease, cleaves peptide bonds at carbonyl group trytophan |
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Term
| Albumin, where is it produced? |
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Definition
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Term
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Definition
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Term
| What is albumin a carrier for? |
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Definition
| Bilibrubin, osmotic regulator, fatty acids |
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Term
| Increase in albumin signifies? |
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Definition
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Term
| Decrease in albumin signfies? |
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Definition
| pregnancy, infants, older adults |
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Term
| Kidney failure is indicated by what level of albumin? |
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Definition
| decrease level of albumin |
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Term
| What is column chromatography? |
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Definition
| seperation of albumin as it is passed through resin |
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Term
| How do you get albumin off the beads? |
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Definition
| change the ph, salt and ion concentration |
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Term
| Anionic resins binds what? |
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Definition
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Term
| Cationic resins binds what? |
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Definition
DEAE has weak positive charge, a weak anionic exchanger will bind negative charge |
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Term
| DEAE diethlyaminoethyl, what kind of charge does it have? what is attracted to it? |
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Definition
| Positive charge, weak charges attracted to it |
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Term
| name a cationic exchanger |
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Definition
|
|
Term
| What does a cationic exchanger attract? |
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Definition
| attracts more negative charge proteins |
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Term
| what does a anionic exchanger seperate? |
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Definition
negative charged DEAE is a cationic resin It is a positve gel Will seperate negative particles anionic exchanger |
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Term
| Carboxyl Methyl resin, what type of exchanger? |
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Definition
CM is anionic resin it is negatively charged will attract positve particles Cationic exchanger |
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Term
| What do you use if you want to bind positive molecules? |
|
Definition
To bind positive particles you need a negative matrix Carboxly Methly is negative. It is a cationic exchanger |
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Term
| For LDH5 what would you use to seperate it? |
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Definition
| LDH 5 is more positive, so you want a negative exchanger, carboxly methy-cationic |
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Term
| To set buffer more negative should you increase or decrease pH? |
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Definition
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|
Term
| To make buffer more positive you would increase or decrease? |
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Definition
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Term
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Definition
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Term
|
Definition
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Term
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Definition
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Term
|
Definition
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Term
|
Definition
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Term
| How will albumin be eluted off the affi-blue gel? |
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Definition
|
|
Term
| What is western blotting? |
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Definition
| transfer of protein after gel electrophoresis and viewing of it using antibody:antigen interaction |
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Term
|
Definition
| blotting paper used by western blot |
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Term
| What is used to amplify a secondary antibody? |
|
Definition
| horseradish peroxidasde or alkalne phosphatase |
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Term
|
Definition
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Term
|
Definition
| antibody produced by b-cells |
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Term
|
Definition
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Term
|
Definition
| a group of decendent cells from a specific antibody |
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Term
|
Definition
| substance that cause body to create antibody |
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Term
|
Definition
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Term
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Definition
| protein made in response to an antigen |
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Term
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Definition
| generic term used for serum because it contains antibodies |
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Term
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Definition
| epitopes on t-cells, illicit immune response |
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Term
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Definition
| small foreign substance too small to illicit immune response, needs carrier protein |
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Term
|
Definition
| injected with polyclonal antibody to boost immune response |
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Term
|
Definition
| use if monoclonal cells infused with cancerous myelom cells to produce indefinitly |
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Term
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Definition
| protein activated by t-cells to turn it into activated t-cells |
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Term
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Definition
| t-and-b cells can differentiate into memory cells |
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Term
| where are b-cells formed? |
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Definition
| in bone marrow then migrate to lymphoid organs |
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Term
| Some antigens produce antibody response |
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Definition
| polysacchrides, lipopolysacchrides |
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Term
| Where are the antibody binding sites?? |
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Definition
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|
Term
| Which antibody fixes complements to drive cell lysis |
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Definition
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Term
| which antibody is first produced as a response to antigen? |
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Definition
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Term
| Enhance phagocytosis; neutralize toxins & viruses; protects fetus & newborn |
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Definition
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Term
| Major antibody of the recall response |
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Definition
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Term
Mucosal protection/ body secretions
Half life 6 days |
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Definition
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|
Term
Antiparasitic - lysis of parasitic worms
Allergic reactions; On mast cells and basophils, in blood |
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Definition
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Term
| In a secondary response you produce more of which antibody? |
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Definition
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|
Term
| Transplacentally or via colostrum (mother’s milk) |
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Definition
| naturally acquired passive immunity |
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Term
| Injection of Ag (vaccination) |
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Definition
| Artificially acquired active immunity |
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Term
| This is the process of destroying B and T cells that react to self antigens |
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Definition
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|
Term
| What is ELISA used to measure? |
|
Definition
| hormones, thyroid, insulin |
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