Term
1) substitution with less toxic chemicals
2) smallest amount of material |
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Definition
| In the video about assessing toxic risks, what 2 guides should you use when planning a new experiment: |
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Term
| the dose, duration, frequency, and route of exposure |
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Definition
| The extent of exposure to a toxic compound is determined by: |
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Term
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Definition
| LD50 measures acute or chronic toxicity? |
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Term
| Tells co-workers, put up sign saying "EMS in use", tell co-workers to ewar gloves when reaching into hood. |
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Definition
| When the very toxic compound EMS was in use in the hood, and the researcher left, what did she do do? |
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Term
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Definition
| Centrifugation hazards: rotors are designed to run at a maximum ______ with loads at a specific weight. It is also critical to make sure you have a ____ that fits correctly in the rotor. |
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Term
| aersol; biological safety cabinet |
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Definition
| When centrifuging, first decide what level containment is needed. When centrifuging infectious agents always 1) use _________ containment tubes and rotors and 2) load and unload in a ____________ . |
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Term
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Definition
| When working with infectious agents, wait ____ minutes after the centrifuge rotor stops before opening door and if there's a spill, close the door and plan your cleanup. |
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Term
| salts; de-ionized water; logs |
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Definition
| Corrosion or metal fatigue can cause rotor failure so rotors must be maintained and retired properly. Important steps to prevent rotor failure include keeping rotors clean and dry, wash immediately if spills occur or if ____ or corrosives are used, rinse with __________, inspect for pitting, color change and ALWAYS maintain the rotor _____ on an ultraspeed rotor. |
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Term
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Definition
| In the Chemical hazards video, they demonstrated a person equilibrating phenol: This chemical is ___________ and causes serious _________ on contact with skin. |
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Term
| penetrate the skin; inhalation |
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Definition
| Adding chloroform enhances phenol's abilty to ____________ ________. Chloroform also causes cancer, organ damage, and is toxic by _________ and skin absorption |
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Term
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Definition
| When extracting proteins from DNA with phenol/chloroform use a ________ _________!! Empty in appropriate waste container, DON'T ______! |
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Term
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Definition
| The beta emitter ____ can not be detected by a GM counter and it is hazardous by ingestion. |
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Term
| aerosol; screw; container |
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Definition
| To prevent radioactive contamination, you should use: _________ resistant pipet tips _________ top eppendorf tubes for heating and centrifuging Regularly check gloves, equipment and centrifuge Double glove in case you contaminate your hand Use a secondary ___________ in water bath |
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Term
| prior; aerosol; bleach; alcohol; biosafety level 2 |
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Definition
| When performing tissue culture taking these steps will decrease your exposure to potentially infectious agents like HIV and HPV: make sure everything is in the hood ________ to beginning use __________ proof pipets disposable pipets are placed in a container with ________ solution for decontamination the hood is decontaminated with ____________, then 0.5% iodine solution When working with human cells and tissues use _________ practices and techniques, avoid aerosols or splashes and the vaccuum trap must contain __________. |
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