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bio lab exam 2
last four labs
148
Biology
Undergraduate 2
04/10/2012

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Term
DNA
Definition
Deoxyribonucleic Acid
Term
What does DNA carry?
Definition
it carries each cell's genetic information
Term
Genes
Definition
segments of DNA that carry the code for making a molecule or regulating a cellular function
Term
what kind of strands does DNA structure have?
Definition
-two anti-parallel strands
-complementary strands
Term
what direction does dna go in?
Definition
5'-3' direction
Term
what are the two purines in DNA
Definition
adenine
guanine
Term
what are the two pyrimidines
Definition
thymine
cytosine
Term
what is chargaff's rules?
Definition
1:1 ratio of pyrimidine and purine bases
Term
genetic engineering
Definition
the ability to alter the genetics of organisms through DNA technology
Term
nucleotides
Definition
building-block molecules of DNA
Term
what are nucleotides made up of?
Definition
a phosphate group, a 5-carbon sugar molecule and a ringed nitrogen-containing molecule referred to as a "base" or nitrogenous base
Term
Restriction enzymes
Definition
special enzymes that chop up DNA molecules at certain recognition sites known as restriction sites

-'molecular scissors'
Term
restriction endonuclease
Definition
recognizes a specific sequence of nucleotides
Term
how will a person's DNA sequences look when they're chopped up?
Definition
the pieces will exhibit different patterns when they are separated
Term
gel electrophoresis
Definition
a process that separates DNa fragments by size and (charge) weight by using an electric field and represents them as bands
Term
what does the enzyme EcoRI recognize?
Definition
GAATTC and cuts between G and A
Term
what kind of matrix is used for DNA samples in the gel electrophoresis?
Definition
agarose matrix
Term
what kind of matrix is used for DNA samples in the gel electrophoresis?
Definition
agarose matrix
Term
what does a low agarose concentration allow?
Definition
resolution of larger DNA fragments
Term
what do higher concentrations of agarose funciton?
Definition
they facilitate separation of small DNA fragments
Term
DNA differences used as genetic markers can be used for what?
Definition
genetic fingerprinting (DNA profiling, testing, typing)
Term
individual-specific patterns, "fingerprints"
Definition
created by highly polymorphic regions that have short repeated sequences of DNA
Term
VNTRs
Definition
variable number tandem repeats; 2-50 repeated nucleotides
Term
STRs
Definition
short tandem repeats, often tetra- or penta-nucleotide repeates; microsatellites
Term
what are VNTRs and STRs function?
Definition
frequently repeated sequences of DNA in introns (the non-coding regions of the genome)
Term
RFLP
Definition
restriction fragment length polymorphism= first profiling technique
Term
transgenic orgnaisms
Definition
organisms that have had foreign DNA segments or genes inserted into their cells, in other words, have undergone genetic engineering
Term
GMOs
Definition
genetically modified organisms
Term
what are the three components to the cardiovascular system?
Definition
blood, heart, blood vessels
Term
the blood vessels transport blood to and from each part of our body through ___ and ___.
Definition
arteries and veins
Term
what are the four chambers of the heart?
Definition
2 atria= right and left; receive venous blood
2 ventricles= right and left; eject blood into pulmonary arteries and aorta
Term
pericardium
Definition
tough, fibrous sac that protects the heart on the outside while anchoring it to the surrounding structures. (dense connective tissue)
Term
epicardium
Definition
outer layer composed of epithelial and connective tissue (serous membrane)
Term
myocardium
Definition
thick layer of cardiac muscle tissue
Term
endocardium
Definition
thin inner layer continuous with the endothelium of blood vessels
Term
endocardium
Definition
thin inner layer continuous with the endothelium of blood vessels
Term
septum
Definition
muscular partition that separates the right and left sides of the heart
Term
aorta
Definition
the body's largest artery
Term
atrioventricular (AV) valves
Definition
located between the atria and the ventricles
Term
tricuspid valve
Definition
between the Right atrium and the right ventricle
Term
bicuspid vavle (mitral)
Definition
between the left atrium and left ventricle
Term
semilunar valves
Definition
aortic= between the aorta and the left ventricle
pulmonary= between the pulmonary artery and the right ventricle
Term
what are the two reasons why cardiac muscle is special?
Definition
1) it does not connect to bone like skeletal muscle
2) it contracts continuously to propel blood through the blood vessels to every region of the body
Term
where is the heart's natural position?
Definition
the chest or thoracic cavity
Term
when the blood returns to the heart from the body's tissues, it enters the heart at the ____.
Definition
right atrium
Term
after it enters the right atrium, where does the blood pass through?
Definition
the right ventricle
Term
what does the right ventricle do?
Definition
it pumps blood through a second valve and into the artery leading to the lungs
Term
When blood returns from the lungs to the heart, it enters ___
Definition
the left atrium
Term
after the left atrium where does the blood pass through?
Definition
the left ventricle
Term
where does the right side of the heart rest on?
Definition
the diaphragm
Term
there are 3 large blood vessels. Where and what are they?
Definition
one is in the center, the aorta. which is situated between the "superior vena cava" on the right side and the "pulmonary trunk" on the left side of the heart
Term
what separates the left and right ventricles?
Definition
the interventricular septum
Term
aortic arch
Definition
the area where the aorta arches over the pulmonary trunk
Term
pulmonary circulation
Definition
pulmonary arteries carry deoxygenated blood to the lungs, while pulmonary veins carry oxygenated blood back to the heart.
Term
what is the flow of the pulmonary circulation?
Definition
Right ventricle to the lungs to the left atrium
Term
systemic circiulation
Definition
aorta and its branches carry oxygenated blood to tissue cells, while inferior and superior venae cavae and their branches carry deoxygenated blood back to the heart.
Term
what is the flow of systemic circulation
Definition
left ventricle to tissue cells to right atrium
Term
infeiror vena cava
Definition
brings blood to the heart from the inferior or lower part of the body
Term
superior vena cava
Definition
brings blood to the heart from teh superior or upper part of the body
Term
what does the pulmonary semilunar valve regulate?
Definition
the flow of blood into the pulmonary trunk (right side)
Term
what does the aortic semilunar valve regulate?
Definition
the flow of blood into the aorta (on the left side)
Term
what is the right atrioventricular (AV) valve for?
Definition
one valve that regulates teh floow of blood from the atrial chambers to the ventricular chambers. (on the right side)
also known as the tricuspid valve
Term
what is the left atrioventricular (AV) valve?
Definition
a valve that regulates the flow of blood from teh atrial chambers to teh ventricular chambers on teh left side.
also known as the mitral or bicuspid valve
Term
chordae tendineae
Definition
anchor the AV valves
Term
papillary muscles
Definition
the contractions of these powerful ventricular muscles provide the blood pressure to transport blood from teh heart to the extremities: the fingers and toes.
Term
what is the typical heart rate at rest?
Definition
75 bpm
Term
what does pulse refer to?
Definition
alternating surges of the pressure in an artery, which correlates to the contraction and relaxation of the left ventricle
Term
cardiac cycle
Definition
the repeating pattern of contraction and relaxation of the heart
Term
systole
Definition
ventricles contract
Term
diastole
Definition
ventricles relax
Term
what does teh 1st "lub" sound refer to?
Definition
the closing of the AV valves
Term
what does teh 2nd "dub" sound refer to?
Definition
the closing of the semilunar valves
Term
what is the typical healthy resting heart rate in adults?
Definition
60-80 beats/minute
(everything between 60-100bpm is normal)
Term
what are athletes' heart rates?
Definition
between 40-60 bpm
Term
bradycardia
Definition
consistently below 60 bpm
Term
tachycardia
Definition
consistently above 100 bpm
Term
sphygmomanometer
Definition
measures the changing blood pressure associated with the pulse
Term
what is blood pressure?
Definition
the force that blood exerts on teh wall of the blood vessel
Term
what is blood pressure measured in?
Definition
mm Hg
Term
how is blood pressure measured?
Definition
indirectly on the brachial artery when a pressure cuff is inflated and deflated
Term
systolic pressure
Definition
the peak pressure in the arteries during the contraction of ventricles
Term
diastolic pressure
Definition
the lowest pressure at the resting phase of the cardiac cycle- relaxation of ventricles and atria
Term
what is the equation for pulse pressure
Definition
systolic P-diastolic P
Term
what is the resting pulse pressure in healthy adults?
Definition
~40 mmHg
Term
during exercise, what prgressively increases and what remains the same?
Definition
systolic pressure increases, diastolic pressure remains the same
Term
what are the four different types of blood?
Definition
A, B, AB, O
Term
what is the universal donor?
Definition
O-
Term
what is the universal recipient?
Definition
AB+
Term
what are antigens?
Definition
identifying proteins

antigen A, B, Rh
Term
what antibodies are formed for type a blood
Definition
antibodies against B
Term
what antiobodies are formed for type B blood
Definition
against A
Term
type Ab blood contains...
Definition
both antigens A and B but doesnt form antibodies against either
Term
Type O blood
Definition
doesnt contain A or B, but forms antibodies against both
Term
what is the Rh factor?
Definition
(Rhesus factor)the name given to another antigen present in red blood cells. People with Rh+ blood have the antigen, while those with Rh- blood dont
Term
agglutination
Definition
the clumping of blood cells
Term
Rh+ persons cannot donate blood to?
Definition
Rh- person because anti-Rh antibodies produced only after Rh- person is exposed to Rh+ blood
Term
what disease comes about between an Rh- mom and Rh+ baby?
Definition
Rh disease or hemolytic disease
Term
what prevents the mom's sensitization to the Rh antigen and production of anti-Rh antibodies?
Definition
injections of RhoGAM (Rh0(D) Immune Globulin
Term
what are the kidneys responsible for?
Definition
the elimination of most of teh waste products from the metabolism of nitrogen containing compounds such as urea and creatinine from protein catabolism, as well as ketone bodies from fat catabolism.
-maintain homeostasis of electrolyte conc., fluid balance and acid-base balance
Term
what is the difficulty of this kideny funciton?
Definition
the kidney also reabsorbs molecules such as glucose, amino acids, and bicarbonate that ar enecessary fro normal body function
Term
what are structures in the urinary system?
Definition
2 kidneys, 2 ureters, bladder, urethra (renal artery and renal vein)
Term
what is osmoregulation
Definition
water balance (ADH, aldosterone)
and salt (Na+ and K+) concentraitons (aldosterone)
Term
what is the acid-base balance
Definition
pH of 7.4
Term
what hormone gets secreted?
Definition
erythropoietin (EPO), calcitriol (vitamin D)
Term
what enzyme gets secreted?
Definition
renin (which regulates the mean arterial BP)
Term
Adrenal glands secrete...
Definition
hormone aldosterone
Term
why is the left kidney slightly higher than the right?
Definition
due to the large area occupied by the liver
Term
ADH (antidiuretic hormone) causes what?
Definition
reapsorption of water and thus reduces urine volume and increases its concentration
Term
Urine passes from each kidney to the __ through a ___. The upper end of each ureter is enlarged to form a funnel-like __. The lower end of each ureter enteres the posterior surface of the bladder. Leading from the urinary bladder to the exteiror is a short tube, the ___.
Definition
urinary bladder
ureter
renal pelvis
urethra
Term
the exit of urine form the bladder is called
Definition
micturition
Term
sphincter vesicae
Definition
a smooth muscle sphincter that is near the exit of the bladder
Term
what are the steps of excretion?
Definition
filtration (~180L/day), reabsorption (~178-179 L/day), secretion (urine= excess water, salts, urea), excretion
Term
the kidney is composed of three regions
Definition
the renal cortex, renal medulla, and renal pelvis
Term
what are calyces?
Definition
they are short tubes that receive urine from the renal papillae; they empty into the large renal pelvis
Term
what is the nephron?
Definition
the basic functional unit of the kidney
Term
80% of the nephrons are located in the
Definition
cortex (cortical nephrons)
Term
the remainder of the nephrons are located
Definition
partially in the cortex and partially in the medulla. (juxtamedullary nephrons)
Term
what parts compose each nephron
Definition
1) the glomerulus= a tightly woven, highly permeable capillary bed at the end of an arteriole
2) the renal tubule=a bent convoluted tube composed of kidney cells
Term
what does the central nervous system consist of?
Definition
the brain and the spinal cord
Term
what does peripheral nervous system consist of?
Definition
cranial nerves (12 pairs), spinal nerves (31 pairs), and ganglia (aggregations of cell bodies)
Term
what does your nervous system do?
Definition
interprets messages and decides how to respond
Term
what are three major regions of the brain?
Definition
cerebrum, cerebellum, and brain stem
Term
what protects the brain?
Definition
skull, meninges, and cerebrospinal fluid
Term
what are the meninges
Definition
triple layer of membranes that covers the brain
Term
what are the meninges
Definition
triple layer of membranes that covers the brain
Term
neurons
Definition
basic structural and functional units of the nervous system
-specialized cells that conduct electrochemical impulses called nerve impulses or action potentials
Term
what is the cell structure:
Definition
dendrites, cell body, and an axon
Term
neuroglia
Definition
support neurons (astrocytes, oligodendrocytes, Schwann cells, radial glia)
Term
what are the three different types of neurons?
Definition
sensory neurons, motor neurons, and interneurons
Term
sensory neurons
Definition
from stimulus receptors (touch, vision, sound) to the CNS
Term
motor neurons
Definition
from teh CNS to the muscles and glands (effectors)
Term
interneurons
Definition
connector neurons or association neurons
Term
what are the 5 general senses?
Definition
temperature, touch, pain ,pressure, and body position
Term
what are the 5 special senses?
Definition
smell, taste, hearing, vision, and equilibrium
Term
reflexes
Definition
responses to stimuli that do not require the brain's involvment
Term
nerve impulse
Definition
information carried along nerves by electrical charges
Term
neurotrasnmitters
Definition
nerve impulses that are transmitted between neurons and from neurons to other cells by chemical stimuli
Term
what happens when a neuron is stimulated?
Definition
sodium channels open and positively charged ions flow into the cell. A domino effect known as an action potential occurs as the positively charged sodium ions move toward neighboring sodium channels, causing them to open and let in additional sodium
Term
synapse
Definition
adjacent neurons that are not directly attached to each other, they are separated by small gaps
Term
what is a reflex arc
Definition
a neural pathway that consists of a receptor, sensory neuron, integration center, motor neuron and effectors
Term
monosynaptic
Definition
only one synapse formed in the spinal cord
Term
what is the axon of a neuron insulated with?
Definition
a material called myelin sheath, made up of Schwann cells
Term
myelinated nervous tissue appears
Definition
white
Term
unmyelinated tissue is...
Definition
gray
Term
what is the vertebrate brain divided into?
Definition
the forebrain, midbrain, and the hindbrain
Term
what does the forebrain consists of?
Definition
thalamus, hypothalamus, hippocampus, pineal gland, and pituitary gland
Term
the midbrain is concerned with responses of the eye and ear
Definition
Term
the hindbrain is composed of?
Definition
the cerebellum and medulla
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