Term
Cells use chemical energy stored in organic molecules to regenerate _____, which powers work |
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Definition
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includes both aerobic* and anaerobic* respiration but is often used to refer to aerobic respiration |
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Definition
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Energy is captured in the form of____ and lost as ___ |
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Definition
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in ____ a substance loses electrons |
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Definition
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in ______ a substance gains electrons (amount of positive charge is reduced) |
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Definition
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Chemical reactions that transfer electrons between reactants are called |
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Definition
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An electron loses ________ energy when it moves from a less electronegative atom toward a more electronegative atom. |
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Definition
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The electron donor is called the _____ agent |
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Definition
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The electron receptor is called the _____ agent |
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Definition
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Coenzyme _____ is a key electron carrier in redox reactions. |
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Definition
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Term
NADH passes the electrons to the |
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Definition
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Term
3 stages of cellular respiration |
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Definition
glycolysis, citric acid cycle and oxidative phosphorylation |
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Term
breaks down glucose to pyruvate |
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Definition
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finishes breaking down glucose |
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Definition
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Term
oxidative phosphorylation |
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Definition
accounts for hte ATP synthesis because its powered by redox reactions |
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Term
breaks down pyruvate to alcohol or lactic acid |
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Definition
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Term
substrate level phosphorylation involves transfer of a |
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Definition
phosphate from a substrate molecule to ADP |
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Term
glycolysis takes place in the ___ |
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Definition
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Term
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Definition
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ATP is a substrate for _____. But_____ is also allosterically inhibited by ATP and activated by AMP |
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Definition
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Term
the electron transport chain _____ generate ATP as it passes electrons from one recipient molecule to hte next |
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Definition
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the # of ATP molecules produced per molecule of NADH is |
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Definition
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the # of ATP molecules produced per molecule of FADH2 is |
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Definition
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difference between lactic acid and alcohol is that in alcohol ___ is produced |
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Definition
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_____ pathways don't have to rely on glucose |
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Definition
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Term
oxidative phosphorylation is the synthesis of ATP dependent on |
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Definition
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Term
The _____ takes place within the mitochondrial matrix |
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Definition
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Term
The ________ and _______ produced by the cycle of glucose metabolism relay electrons extracted from food to the electron transport chain |
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Definition
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Term
The electron carriers that are reduced during the citric acid cycle must be ____ to take part in the cycle again. |
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Definition
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Term
ATP is synthesized by reoxidation of electron carriers in the presence of O2.
this is the process of |
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Definition
oxidative phosphorylation |
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Term
2 stages of oxidative phosphorylation: |
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Definition
electron transport and chemiosmosis |
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Term
The respiratory chain is located in the inner _____ |
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Definition
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Term
During electron transport _______ are also actively transported. |
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Definition
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Term
_____ accumulate in the intermembrane space and create a concentration gradient and charge difference—potential energy! |
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Definition
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Term
Protons diffuse back into the mitochondria through ATP synthase, a channel protein. Diffusion is coupled to ATP synthesis.
this describes |
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Definition
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glucose -> NADH -> electron transport chain -> proton-motive force -> ATP
this is the energy flow of |
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Definition
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Term
how much ATP is made from cellular respiration? |
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Definition
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Term
carry out fermentation or anaerobic respiration and cannot survive in the presence of O2 |
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Definition
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Term
In a _____ anaerobe, pyruvate is a fork in the metabolic road that leads to two alternative catabolic routes |
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Definition
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Term
pathways funnel electrons from many kinds of organic molecules into cellular respiration |
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Definition
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Term
Fatty acids are broken down by _______ and yield acetyl CoA |
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Definition
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Term
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Definition
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An oxidized gram of ____ produces more than twice as much ATP as an oxidized gram of carbohydrate |
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Definition
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Term
in anabolic pathways the body uses ______ to build other substances |
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Definition
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Term
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Definition
oxidative phosphorylation |
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_____ inhibits one of the proteins in the electron transport chain. |
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Definition
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Term
_______ and ATP are coupled, you can't have one without the other |
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Definition
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Term
_____ (Complex V) is a molecular motor also in the lipid bilayer, driven by proton passage through a channel that lets them back in again. ( all occurs in inner membrane of the mitochondria ) |
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Definition
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Term
In concert with this electron transport, ______ are pumped out of the mitochondria into the inter-membrane space by Complexes I, III and IV, creating a higher pH (less acidic) within the innner mitochondrial matrix. |
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Definition
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Term
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Definition
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- specialized fat cells, much richer than normal in mitochondria |
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Definition
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Term
It provides a proton channel from outside to inside, dissipating the proton gradient without making ATP, thus causing the ETC to run faster, creating...heat! |
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Definition
thermogenin...found in brown fat |
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Term
in photosynthesis... Plants take in CO2, produce ______, and release water and O2 |
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Definition
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Term
what is the reverse of cellular respiration? |
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Definition
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Term
-As in the mitochondria, separate membranes inside the organelle contain the proteins involved in electron transport and ATP synthesis. -Accumulation of H+ within a membrane bound space creates a concentration gradient which drives ATP synthesis.
these are characteristics of |
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Definition
both photosynthesis and cellular respiration |
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Term
water is the source of ____ released during photosynthesis |
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Definition
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Term
Photosynthesis is an ______ process; the energy boost is provided by light |
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Definition
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Term
in photosynthesis ____ is oxidized and ____ is reduced |
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Definition
water is oxidized.... carbon dioxide is reduced |
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Term
the type of rays that are most electromagnetic are |
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Definition
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Term
When a photon meets a molecule it can be: Scattered— |
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Definition
photon bounces off the molecule |
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Term
When a photon meets a molecule it can be transmitted= |
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Definition
photon is passed through the molecule |
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Term
When a photon meets a molecule it can be absorbed= |
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Definition
—molecule acquires the energy of the photon. The molecule goes from ground state to excited state |
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Term
____ Absorb in red and blue regions, transfer the energy to chlorophylls—carotenoids and phycobilins |
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Definition
accessory pigments in photosynthesis |
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Term
Energy can pass to another molecule if: Target molecule is very near Orientation is correct Has appropriate structure
the molecules that meet this criteria are called |
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Definition
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Term
the ________ (pigment molecules bound to proteins) funnel the energy of photons to the reaction center |
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Definition
light harvesting complexes |
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Term
photosystem _____ functions first |
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Definition
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Term
when combined _______ & ______ engage in linear electron flow which produces ATP and NADPH using light energy |
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Definition
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Term
Light energy oxidizes water → O2, H+, and electrons.
this happens in photosystem __ |
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Definition
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Term
____________ Uses only photosystem I and produces ATP, but not NADPH |
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Definition
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Term
in cyclic electron flow _____ ____ is released |
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Definition
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Term
_____________ generates extra ATP which funds the Calvin cycle |
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Definition
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Term
Chloroplasts and mitochondria generate ATP by ________, but use different sources of energy |
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Definition
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Term
Mitochondria transfer ______ energy from food to ATP |
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Definition
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Term
chloroplasts transform _______ energy into the chemical energy of ATP |
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Definition
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Term
light reactions generate ATP and increase the potential energy of electrons by moving them from ____ to ____ |
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Definition
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Term
stroma has a _____ H+ concentration |
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Definition
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Term
Cyclic electron flow may protect cells from _______ damage |
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Definition
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Term
The Calvin cycle uses ATP and NADPH to convert ______ to _________ |
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Definition
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Term
For net synthesis of 1 G3P, the calvin cycle must take place _____ times, fixing ___ molecules of CO2 |
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Definition
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Term
Carbon fixation (catalyzed by rubisco) Reduction Regeneration of the CO2 acceptor (RuBP = ribulose bisphosphate)
THESE ARE THE 3 PHASES OF THE |
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Definition
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Term
to generate one _________ , More energy goes into making a molecule of glucose than is taken out in cellular respiration |
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Definition
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Term
On hot, dry days, plants close ____, which conserves H2O but also limits photosynthesis |
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Definition
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Term
hot, arid conditions for carbon fixation are favorable for a process called ____ |
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Definition
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Term
_____ is a response to decreased carbon dioxide and increased oxygen levels when the stomata are closed |
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Definition
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Term
Photorespiration limits damaging products of light reactions that build up in the absence of the _____ |
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Definition
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Term
In many plants, _______ is a problem because on a hot, dry day it can drain as much as 50% of the carbon fixed by the Calvin cycle |
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Definition
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Term
____ & _____ Attach the CO2 to something else using a different enzyme Then peel off the CO2 and send it to the Calvin cycle. as an alternative to photorespiration |
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Definition
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Term
____ plants minimize the cost of photorespiration by incorporating CO2 into four-carbon compounds in mesophyll cells This step requires the enzyme PEP carboxylase |
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Definition
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Term
the enzyme ______ has a higher affinity for CO2 than rubisco does; it can fix CO2 even when CO2 concentrations are low |
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Definition
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Term
These four-carbon compounds are exported to _____ cells, where they release CO2 that is then used in the Calvin cycle |
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Definition
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Term
____ plants open their stomata at night, incorporating CO2 into organic acids...which are then released in the day and used in the calvin cycle |
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Definition
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Term
______ is the basis of inheritance |
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Definition
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Term
When Griffith mixed heat-killed remains of the pathogenic strain with living cells of the harmless strain, some living cells became pathogenic this proves that |
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Definition
DNA can transform bacteria |
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Term
a change in genotype and phenotype due to assimilation of foreign DNA. |
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Definition
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Term
viruses that infect bacteria are called |
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Definition
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Term
viruses are basically DNA (or in some cases RNA) wrapped in a ___ coat |
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Definition
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Term
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Definition
nucleotides: nitrogenous base, phosphate group deoxyribose |
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Term
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Definition
nucleotides: nitrogenous base, phosphate group deoxyribose |
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Term
Erwin Chargaff reported that DNA composition _____ |
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Definition
varies from one species to the next |
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Term
Chargaff’s rule states that in any species there is an equal number of |
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Definition
A and T bases, and an equal number of G and C bases |
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Term
a crucial piece of DNA structure came through ____ |
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Definition
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Term
Rosalind Franklin had also concluded that there were two _____ sugar-phosphate backbones, with the nitrogenous bases paired in the molecule’s interior |
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Definition
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Term
the two guys that used franklin's work in combo with their own to do hte DNA structure are |
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Definition
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Term
It is a double-stranded helix of uniform diameter It is right-handed It is antiparallel
these are the characteristics of |
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Definition
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Term
DNA structure predicts the _____ |
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Definition
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Term
Watson and Crick’s ________ model of replication predicts that when a double helix replicates, each daughter molecule will have one old strand (derived or “conserved” from the parent molecule) and one newly made strand |
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Definition
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Term
the ____ model which was rejected, said that each strand is a mix of old and new |
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Definition
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Term
the ____ model which was also rejected said that the two parent strands rejoin |
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Definition
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Term
THESE ARE THE TWO STEPS OF
1. The double helix is unwound, making two template strands
2. New nucleotides are added to the new strand at the 3′ end and joined by phosphodiester linkages. Sequence is determined by complementary base pairing. |
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Definition
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Term
where the two DNA strands are separated, opening up a replication “bubble” is called the |
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Definition
origins of replication, where replication begins (ori) |
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Term
on the lagging strand, the enzyme DNA ligase forms a ______ bond between 3' OH of the growing strand and the 5'phosphate in front of it |
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Definition
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Term
a Y-shaped region where new DNA strands are elongating at the end of each replication bubble... this is called the |
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Definition
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Term
A eukaryotic chromosome may have _____ of origins of replication |
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Definition
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Term
in DNA replication, Synthesis of the daughter strands proceeds in the ___ to __ direction. |
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Definition
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Term
in DNA replication New nucleotide can only be attached to an available 3’ __ on the ____ |
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Definition
carbon on the deoxyribose |
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Term
____ are enzymes that untwist the double helix at the replication forks |
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Definition
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Term
____ binds to and stabilizes single-stranded DNA until it can be used as a template |
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Definition
single stranded binding protein |
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Term
corrects “overwinding” ahead of replication forks by breaking, swiveling, and rejoining DNA strands |
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Definition
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Term
DNA replication begins with a short ____—a starter strand. The____ is complementary to the DNA template. |
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Definition
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Term
____ -an enzyme—synthesizes the primer one nucleotide at a time. |
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Definition
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Term
new DNA strand begins at the _____ end |
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Definition
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Term
in DNA replication.._________ attaches nucleotides to the 3′ end of the deoxyribose sugar component -Uses the RNA primer as the starting point |
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Definition
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Term
DNA GROWS from the ___ end to the ____ end. |
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Definition
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Term
Nucleotide precursers for DNA = |
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Definition
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Term
What is the difference between ATP, the energy carrier and dATP the adenosine nucleotide building block for DNA? |
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Definition
Deoxyribose sugar in dATP |
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Term
Each nucleotide that is added to a growing DNA strand is a ______ because fo the 3 phosphate groups that were added |
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Definition
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Term
dNTP supplies adenine to ____ and is similar to the ATP of energy metabolism |
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Definition
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Term
As each monomer of dNTP joins the DNA strand, it loses two phosphate groups as a molecule of ___ |
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Definition
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Term
the mechanics of DNA elongation _____ between the two strands |
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Definition
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Term
Leading strand: DNA synthesized continuously. Away from __, towards ______ |
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Definition
away from origin towards replication fork |
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Term
The lagging strand is synthesized as a series of segments called _______, which are joined together by ______ |
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Definition
okazaki fragments which are joined together by DNA ligase |
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Term
Cells have three repair mechanisms: _____ ___ repair ____ repair |
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Definition
proofreading, excision repair and mismatch repair |
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Term
During replication: DNA polymerases _____ newly made DNA, replacing any incorrect nucleotides |
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Definition
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Term
DNA can be damaged by environmental agents such as UV light, x rays, cigarette smoke, etc. When this happens the damage can often (but not always) be repaired by nucleotide ____ repair. |
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Definition
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Term
in ____ repair, A nuclease cuts out and replaces damaged stretches of DNA |
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Definition
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Term
Limitations of ________ create problems for the linear DNA of eukaryotic chromosomes The usual replication machinery provides no way to complete the 5' ends, so repeated rounds of replication produce shorter DNA molecules |
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Definition
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Term
Eukaryote chromosomes have repetitive sequences at the ends called |
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Definition
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Term
_____ are protective and prolong cell division, especially in rapidly-dividing cells, like bone marrow. |
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Definition
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Term
the enzyme _____ contains an RNA sequence—acts as template for telomeric DNA sequences |
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Definition
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Term
the flow of genetic information:
DNA nucleotide sequence --> _____ to the mRNA sequence ---> ____ to amino acid sequence (polypeptide) |
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Definition
transcribed to mRNA sequence ---> translated to the amino acid sequence of polypeptides |
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Term
the genotype is the ____ sequence the phenotype is the ___ sequence |
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Definition
DNA nucelotide= genotype
amino acid=phenotype |
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Term
Archibald Garrod saw a disease phenotype— alkaptonuria—occurring in children who shared more alleles as first cousins.
this investigation gave evidence that genes code for ___ |
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Definition
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Term
____ organisms are Easy to grow or observe; show the phenomenon to be studied. |
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Definition
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Term
Beadle and Edward concluded that Based on their results they developed a ______, which states that each gene dictates production of a specific enzyme.
later revised to |
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Definition
one gene one enzyme hypothesis
one gene one polypeptide |
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Term
Beadle and Edward concluded that Based on their results they developed a ______, which states that each gene dictates production of a specific enzyme. |
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Definition
one gene one enzyme hypothesis |
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Term
what is the central dogma of molecular biology? |
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Definition
DNA transcribes to RNA which translates to a protein |
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Term
In a eukaryotic cell, the _______ separates transcription from translation |
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Definition
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Term
A ____ transcript is the initial RNA transcript from any gene |
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Definition
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Term
The mature mRNA exits the nucleus through the nuclear pores and attaches to ______ and translation of the message into polypeptide begins. |
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Definition
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Term
Single-strand _____ can fold into complex shapes by internal base pairing. |
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Definition
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Term
So ___ nucleotides per amino acid is the smallest possible code that will provide enough information for at least 20 amino acids. |
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Definition
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Term
Any single set of three nucleotides is called a ___ , and the set of all possible three-nucleotide combinations is called ___ or ___ |
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Definition
codon... combos are called the genetic code or the triplet code |
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Term
what part of transcription is for eukaryotes only? |
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Definition
Post-transcription modification |
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Term
Initiation Elongation Termination Post-transcription modification (eukaryotes only)
these are the 4 steps in |
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Definition
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Term
during initiation of transcription ______ catalyzes the synthesis of mRNA, separates the two DNA strands & polymerizes the ribose nucleotides |
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Definition
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Term
DNA sequence that tells RNA polymerase “attach here”. |
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Definition
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Term
RNA polymerase unwinds the DNA and begins ribose nucleotide polymerization
what part of transcription is this describing? |
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Definition
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Term
Nucleotides added in 5’ to 3’ direction (just like DNA synthesis)
what part of transcription is this describing? |
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Definition
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Term
Transcription stops when RNA pol reaches a specific nucleotide sequence on the template: ____ |
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Definition
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Term
only eukaryotes need _____ which mediate the binding of RNA polymerase and the initiation of transcription |
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Definition
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Term
The completed assembly of transcription factors and RNA polymerase II bound to a promoter is called a ____ ____ complex |
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Definition
transcription initiation complex |
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Term
A promoter called a _____ is crucial in forming the initiation complex in eukaryotes |
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Definition
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Term
Transcription progresses at a rate of ___ nucleotides per second in eukaryotes |
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Definition
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Term
A sequence RNA polymerase adds a polyadenylation sequence: AAUAAA
The transcript is released another 10-35 nucleotides past the polyA sequence.
The transcript is a ____ that undergoes additional processing before it leaves the nucleus |
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Definition
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Term
____ consist of a variety of proteins and several small nuclear ribo-nucleoproteins (snRNPs) that recognize the splice sites |
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Definition
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Term
It can form a three-dimensional structure because of its ability to base-pair with itself Some bases in RNA contain functional groups that may participate in catalysis RNA may hydrogen-bond with other nucleic acid molecules
these three reasons are why RNA can function as an ____ |
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Definition
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Term
introns may contain sequences that regulate ___ |
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Definition
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Term
Proteins often have a modular architecture consisting of discrete regions called _____. In many cases, different exons code for the different _____ in a protein |
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Definition
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Term
Exon ______ may result in the evolution of new proteins |
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Definition
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Term
1. Collect the right amino acids tRNA first tRNA carries start codon (Met) 2. Join together mRNA, ribosome and tRNA 3. Bring in tRNAs in order as mRNA is “read” 4. Connect the amino acids 5. Continue until stop codon reached
these are the steps of ___ |
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Definition
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Term
Each carries a specific amino acid on one end and Has an anticodon on the other end |
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Definition
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Term
Flexible pairing at the third base of a codon is called _____ and allows some tRNAs to bind to more than one codon |
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Definition
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Term
this enzyme ___ has to make a correct match between a tRNA and an amino acid in order to have accurate translation |
|
Definition
the enzyme aminoacyl-tRNA synthetase |
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Term
_____ bring together tRNA anticodons with mRNA codons in protein synthesis, and has more rRNA than a protein |
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Definition
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Term
1. A = aminoacyl tRNA binding site Incoming tRNA bringing the next aa in the polypeptide sequence 2. P = peptidyl-tRNA binding site Holds growing polypeptide chain 3. E = exit site tRNA exits the ribosome
these are the 3 ______ sites that ____ attaches to in each sequence |
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Definition
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Term
Termination of translation occurs when a stop codon in the mRNA reaches the ____ site of the ribosome |
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Definition
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Term
The A site accepts a protein called the _____ factor which causes the addition of a water molecule instead of an amino acid. This reaction releases the polypeptide, and the translation assembly then comes apart |
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Definition
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Term
A number of ribosomes can translate a single mRNA simultaneously, forming a ____ which enable a cell to make many copies of a polypeptide very quickly |
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Definition
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Term
Often, translation isn't enough. Polypeptide chains are modified after translation or targeted to specific sites in the cell in order to make a ______ protein |
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Definition
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Term
During and ____ synthesis, a polypeptide chain spontaneously coils and folds into its three-dimensional shape |
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Definition
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Term
besides folding How else can proteins be modified? ____ated or ____lated |
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Definition
methylated or phosphorylated |
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Term
Polypeptide synthesis always begins in the ____. Synthesis finishes there unless the polypeptide signals the ribosome to attach to the ER |
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Definition
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Term
Polypeptides destined for the ER or for secretion are marked by a _____ |
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Definition
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Term
_____ ribosomes make proteins of the endomembrane system and proteins that are secreted from the cell |
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Definition
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Term
can ribosomes switch from free to bound? |
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Definition
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Term
How does cell “know” whether the protein stays in cytoplasm or goes to ER? A ____ binds to the signal peptide |
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Definition
signal-recognition particle (SRP) |
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Term
what is the only exception to the central dogma? |
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Definition
Viruses: Non-cellular particles that reproduce inside cells; many have RNA instead of DNA. Viruses can replicate by transcribing from RNA to RNA, and then making multiple copies by transcription. |
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Term
After infecting a host cell a copy of the viral genome is incorporated into the host’s genome to make more RNA. this type of virus is called a _____ |
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Definition
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Term
are changes in the genetic material of a cell or virus |
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Definition
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Term
are chemical changes in just ONE base pair of a gene |
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Definition
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Term
The change of a single nucleotide in a DNA template strand can lead to the production of an ____ |
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Definition
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Term
_____ mutation: No change in protein. |
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Definition
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Term
____ mutation: Amino acid substitution. |
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Definition
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Term
____ mutation= Stop codon introduced in the middle. |
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Definition
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Term
_____ mutations in proteins are due to insertion or deletions in the DNA. |
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Definition
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Term
The molecular basis of sickle-cell disease: a ___ mutation |
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Definition
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Term
It might be a little counter-intuitive, but ___ frameshift changes can actually cause the sequence to revert back to normal. |
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Definition
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|
Term
This process produces some ATP and carbon dioxide in the mitochondrion. |
|
Definition
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|
Term
The immediate energy source that drives ATP synthesis by ATP synthase during oxidative phosphorylation is the |
|
Definition
H+ concentration across the membrane holding ATP synthase |
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|
Term
Which metabolic pathway is common to both fermentation and cellular respiration of a glucose molecule? |
|
Definition
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|
Term
In mitochondria, exergonic redox reactions |
|
Definition
provide the energy that establishes the proton gradient. |
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|
Term
The final electron acceptor of the electron transport chain that functions in aerobic oxidative phosphorylation is |
|
Definition
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|
Term
What is the oxidizing agent in the following reaction? Pyruvate + NADH + H+ S Lactate + NAD+ |
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Definition
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|
Term
When electrons flow along the electron transport chains of mitochondria, which of the following changes occurs? |
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Definition
The pH of the matrix increases. |
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Term
What provides electrons for the light reactions? |
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Definition
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|
Term
What provides the carbon atoms that are incorporated into sugar molecules in the Calvin cycle? |
|
Definition
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|
Term
The light reactions of photosynthesis supply the Calvin cycle with |
|
Definition
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|
Term
Which of the following does not occur during the Calvin cycle? |
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Definition
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|
Term
In mechanism, photophosphorylation is most similar to |
|
Definition
oxidative phosphorylation in cellular respiration |
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|
Term
Which process is most directly driven by light energy? |
|
Definition
removal of electrons from chlorophyll molecules |
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Term
The site of translation is |
|
Definition
ribosomes in the CYTOPLASM |
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Term
Which of the following does not occur during RNA processing? |
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Definition
mRNA attaches to the small subunit of a ribosome |
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