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studied 2 strains of bacteria in study of heredity and DNA |
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strain of bacteria that causes disease, pathogenic cells |
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strain of bacteria that is non- pathogenic due to lack of outer coating, becomes pathogenic if co- in ejected with dead S cells |
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genetic conversion, ex. R cells to S cells. DNA transforms phenotype not protein |
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technique for finding the 3D structure of crystallized macromolecules, indicated DNA was some helix structure |
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2 nucleotides from opposite strands H bonded together, sequence of bp's hold hereditary info, used as unit of length |
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Watson- Crick base- pairing rules |
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A bonds with T G bonds with C dictated by chemical & x-ray data nucleotides in pair are complementary |
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process of copying DNA in S phase. H bonds between strands broken each single strand serves as the template for synthesis of new strand each DNA molecule is half old and half new |
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deoxynucleoside triphosphate, provide energy for DNA replication |
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place in DNA where strands separate to begin DNA replication. -multiple organs allow large eukaryotic DNA molecules to be copied quickly -each origin= 2 replication forks -forks move in opposite directions -forks connect to complete synthesis -forks contain many enzymes |
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where DNA unwinds and synthesis of new strands occurs. move in opposite directions connect to complete synthesis contain many enzymes |
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unwinds and separates double helix, enzyme |
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begins synthesis with a short stretch of complementary RNA (the primer) enzyme |
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makes new DNA -can NOT start a strand on its own - 5' to 3' direction only -adds nucleotides to 3' of new strand always - stops when hits 5' of RNA primer |
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continuous strand elongation |
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new strand= leading strand for each template strand is made in 1 piece beginning at origin |
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discontinuous strand elongation |
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another strand= lagging strand for each template strand is synthesized in sections |
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replaces RNA primers with DNA nucleotides |
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completes gaps in sugar phosphate backbone |
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