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bio exam 3
ch.16
18
Biology
Undergraduate 2
04/23/2017

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Term
Griffith Experiment
Definition
studied 2 strains of bacteria in study of heredity and DNA
Term
S
Definition
strain of bacteria that causes disease, pathogenic cells
Term
R
Definition
strain of bacteria that is non- pathogenic due to lack of outer coating, becomes pathogenic if co- in ejected with dead S cells
Term
transformation
Definition
genetic conversion, ex. R cells to S cells. DNA transforms phenotype not protein
Term
X- ray Crystallography
Definition
technique for finding the 3D structure of crystallized macromolecules, indicated DNA was some helix structure
Term
Base Pair (bp)
Definition
2 nucleotides from opposite strands H bonded together, sequence of bp's hold hereditary info, used as unit of length
Term
Watson- Crick base- pairing rules
Definition
A bonds with T
G bonds with C
dictated by chemical & x-ray data
nucleotides in pair are complementary
Term
DNA Replication
Definition
process of copying DNA in S phase.
H bonds between strands broken
each single strand serves as the template for synthesis of new strand
each DNA molecule is half old and half new
Term
dNTP's
Definition
deoxynucleoside triphosphate, provide energy for DNA replication
Term
origin of replication
Definition
place in DNA where strands separate to begin DNA replication.
-multiple organs allow large eukaryotic DNA molecules to be copied quickly
-each origin= 2 replication forks
-forks move in opposite directions
-forks connect to complete synthesis
-forks contain many enzymes
Term
replication fork
Definition
where DNA unwinds and synthesis of new strands occurs.
move in opposite directions
connect to complete synthesis
contain many enzymes
Term
helices
Definition
unwinds and separates double helix, enzyme
Term
primase
Definition
begins synthesis with a short stretch of complementary RNA (the primer) enzyme
Term
DNA Polymerase III
Definition
makes new DNA
-can NOT start a strand on its own
- 5' to 3' direction only
-adds nucleotides to 3' of new strand always
- stops when hits 5' of RNA primer
Term
continuous strand elongation
Definition
new strand= leading strand for each template strand is made in 1 piece beginning at origin
Term
discontinuous strand elongation
Definition
another strand= lagging strand for each template strand is synthesized in sections
Term
DNA Polymerase I
Definition
replaces RNA primers with DNA nucleotides
Term
DNA ligase
Definition
completes gaps in sugar phosphate backbone
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