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ability to promote change or do work |
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due to structure or location |
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energy in molecular bonds |
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difference in ion concentration |
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- Law of conservation of energy - Energy cannot be created nor destroyed - Can be transformed from one type to another |
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First law of thermodynamics |
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transfer or transformation of energy from one form to another increases entropy or degree of disorder of a system |
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Second law of thermodynamics |
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usable energy + unusable energy |
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Energy transformations involve an increase in... |
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a measure of the disorder that cannot be harnessed to do work |
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free energy or amount of energy for work |
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entropy or unusable energy |
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absolute temperature in Kelvin |
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occur without input of additional energy |
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Key factor is the free energy change |
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agent that speeds up the rate of a chemical reaction without being consumed during the reaction |
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protein catalysts in living cells |
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RNA molecules with catalytic properties (discovered in 1980s) |
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allows molecules to get close enough to cause bond rearrangement |
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where bonds are stretched in activation energy |
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stretched to bond with other molecules |
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1. Large amounts of heat 2. Using enzymes to lower activation energy |
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How to overcome activation energy |
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Location where reaction takes place |
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reactants that bind to active site |
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formed when enzyme and substrate bind |
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specificity for a substrate |
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plateau where nearly all active sites occupied by substrate |
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Velocity of reaction near maximal rate (amount of product per unit) |
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Substrate concentration at which velocity is half maximal value = 1/2 V max |
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molecule binds to active site; inhibits ability of substrate to bind; Km increases - more substrate needed |
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Lowers V max without affecting Km; inhibitors binds to allosteric site |
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small molecules (non-protein) permanently attached to the enzyme |
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usually inorganic ion that temporarily binds to enzyme |
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organic molecule that participates in reaction but left unchanged afterward |
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Result in breakdown and are exergonic |
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Promote synthesis and are endergonic Must be coupled to exergonic reaction |
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1. Substrate-level phosphorylation 2. Chemiomiosis |
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Electron removed from one molecule is added to another |
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Cells synthesize molecules only when needed |
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Molecules are broken down when needed for energy or no longer required |
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1. Gene regulation 2. Cellular regulation 3. Biochemical regulation |
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Regulation of metabolic pathways |
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time for 50% of molecules to be broken down |
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protein that attaches to unwanted protein |
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Nicotinamide adenine dinucleotide phosphate |
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Definition
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Nicotinamide adenine nucleotide |
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process by which living cells obtain energy from organic molecules |
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Primary aim to make ATP and NADH |
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can occur with our without oxygen |
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1. energy investment 2. cleavage 3. energy liberation |
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With large amounts of ATP, ATP will bind to allosteric site in phosphofructokinase, which makes enzyme inactive, thus preventing the breakdown of glucose |
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Breakdown of pyruvate to an acetyl group |
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Oxidative phosphorylation |
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Electron transport chains |
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group of protein complexes and small organic molecules embedded in the inner mitochondrial membrane |
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help move things efficiently without requiring energy |
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chemical synthesis of ATP as a result of pushing H across a membrane |
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damage DNA; can cause mutation |
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Chemical reactions occur here... |
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result in breakdown and are exergonic |
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promote synthesis and are endergonic; must be coupled to exergonic reaction |
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for environments that lack oxygen or during oxygen deficits |
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1. use substance other than O2 as final electron acceptor in electron transport chain 2. produce ATP only via substrate-level phosphorylation |
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Definition
2 strategies of anaerobic respiration |
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Donates e- and forms free radicals |
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Definition
Why is a buildup of NADH problematic? |
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breakdown of molecules without net oxidation |
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synthesis and breakdown of molecules with are essential for cell structure and function |
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synthesis of molecules secondary metabolites that are not necessary for cell structure and growth |
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phenolics, alkaloids, terpenoids, polyketides |
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4 categories of secondary metabolites |
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antioxidants with intense flavors and smells |
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bitter-tasting molecules for defense |
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energy within light is captured and used to synthesize carbohydrates |
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Calvin cycle and light reaction |
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two processes of photosynthesis |
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regions on the surface of the Earth and in the atmosphere where living organisms exist |
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must eat food, organic molecules from their environment, to sustain life |
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make organic molecules from inorganic sources |
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use light as a source of energy; green plants, algae cyanobacteria |
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majority of photosynthesis occurs in leaves in this part of a leaf |
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carbon dioxide enters and oxygen exits leaf |
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organelles in plants and algae that carry out photosynthesis |
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third membrane of the chloroplast; contains pigment molecules |
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fluid filled region between thylakoid membrane and inner membrane |
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occurs in stroma; uses ATP and NADPH to incorporate CO2 into organic molecules |
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type of electromagnetic radiation; travels as waves; also behaves as particles- photons |
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captured light energy can be transferred to other molecules to ultimately produce energy intermediates for cellular work |
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ATP and NADPH used to make carbohydrates; CO2 incorporated into carbohydrates |
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