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BIO 230 Microbiology II
General principles of bacteriology and their relationships to pathogenicity and control.
96
Biology
Undergraduate 2
07/15/2009

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Term

Maintain shape and size of bacteria and prevent bursting of the cell.

 

Definition
Function of the cell wall in bacteria.
Term

Peptidoglycan (PG)

Protein-sugar complex

Definition
Main chemical composition of cell wall in bacteria.
Term

Lipoproteins (LP)

 

Lipopolysaccharide (LPS)

Definition
Two additional chemicals that may be found in cell wall of bacteria.
Term

Gram Positive (G+)

 

Gram Negative (G-)

Definition
Two main types of bacteria based on cell wall contents.
Term
Gram positive (G+)
Definition
Bacteria with cell walls consisting of a single thick layer of PG.
Term
Gram Negative (G-)
Definition
Bacterial cells that have cell walls consisting of a thin layer of PG surrounded by layers of LP and LPS.
Term
Gram negative
Definition
E-coli and salmonella are examples of __________ (G+/G-) bacteria.
Term

Outer layer (LPS layer) of a G- cell wall releases endotoxin when the bacteria dies.

 

G+ cell wall does not cause harm.

Definition
Role the cell wall has in pathogenicity.
Term
Endotoxin
Definition
A poison that is released from the cell wall of a G- bacteria upon death of the bacteria.
Term

Stimulates inflammation

Causes damage to the animal cell membrane

Causes cell necrosis

Definition
General effects of endotoxin
Term
Inflammation
Definition
Main way the body protects itself from harm/infection.
Term
Gastroenteritis- causes fluid loss, diarrhea, dehydration, shock.
Definition
Effects of endotoxin on digestive tract.
Term

Inflammation of the urethra, etc.

Urethritis, cystitis, nephritis, pyelitis.

Definition
Effects of endotoxin on urinary tract.
Term
G- bacteria
Definition
Most common cause of UTI's - 70%
Term
E. coli
Definition
Number 1 bacteria in our environment (most prevalent).
Term

Men - urethritis, prostatitis, epididymitis

 

Women - cervicitis, pelvic inflammatory disease (PID)

Definition
Effect of endotoxin in STD's such as chlamydia and gonorrhea.
Term
Causes scar tissue which can lead to sterility.
Definition
Effects of epididymitis on men.
Term
Damages fallopian tubes and ovaries, which can cause sterility or ectopic pregnancy.
Definition
Effects of PID on women.
Term
Damages lining of blood vessels, which can lead to hemorrage or septic shock.
Definition
Effects of endotoxin from G- bacteria in the blood vessels.
Term

Post surgical contamination

Traumatic Wounds

Ruptured appendix

Definition
Ways in which G- bacteria can be introduced to the blood supply.
Term

1. Car crash

 

2. Gun shot wound

Definition
Top two causes of traumatic wounds
Term
Penicillin and cephalosporins stop PG formation in G+ bacteria cell walls. Rarely used to treat G- bacteria.
Definition
Role the cell wall plays in treatment of bacterial infections.
Term

Serves as a semipermeable barrier

 

Site of cell wall repair

 

Site of energy production

 

Plays a role in cell division

Definition

Functions of Bacterial Cell Membrane

 

Term
Asexually through binary fission.
Definition
How bacteria reproduce.
Term

Helps separate DNA

 

Forms a new cross wall/septum

Definition
How bacterial cell membrane participates in cell division.
Term
None
Definition
Role a bacterial cell membrane plays in harming the person it infects.
Term
Cell membranes are damaged by antibiotics, antiseptics, disinfectants, soaps, carbolic acids.
Definition
Role cell membranes play in treatment of infection.
Term

polymyxin B
(aerosporin - "neosporin")

 

Bacitracin

Definition
Antibiotics that harm cell membranes.
Term
Antiseptics and Disinfectants
Definition
Not safe to use in/on the body, but can damage cell membrane in bacteria.
Term
lathering/scrubbing action, scrubs and ruptures the membrane.
Definition
How soaps and detergents work as antibacterials.
Term
synthetic soaps/detergents
Definition
Use chemical action to damage membranes without scrubbing.
Term

Quaternary Ammonium Compounds (Quats)

Iodaphor

Tincture Soap

Definition
Examples of synthetic soaps.
Term
Quaternary Ammonium Compound
Definition
Zephiran
Term
Iodaphor
Definition
synthetic soap that contains iodine
Term
Tincture Soap
Definition
Synthetic soap that contains alcohol.
Term

lysol, osyl, phenol, cresol

 

hexachlorophene

Definition
Examples of carbolic acids that are used to damage cell membrane.
Term
hexachlorophene
Definition
Carbolic acid that contains soap and phenol.
Term
phisohex
Definition
Hexachlorophene that was used to treat staph infection in newborn babies and pimples.
Term
200 mph
Definition
Average speed a bacteria moves.
Term
Motility - movement of the bacteria.
Definition
Function of flagella.
Term
Made of protein  - flagellin.
Definition
Chemical composition of flagellum.
Term
polar monotrichous
Definition
Bacteria with one flagellum.
Term
polar lophotricous
Definition
Bacteria with several strands of flagella originating from the same point.
Term
peritrichous
Definition
bacteria with many flagella all the way around it
Term

In response to chemicals - chemotaxis

 

In response to light - phototaxis

 

In response to magnetic fields.

Definition
Reasons bacteria move.
Term

L Ring

P Ring

S Ring

M Ring

Definition
Flagella attach to bacteria by 4 anchors/rings called...
Term

L Ring

 

Definition
Anchor attached to the LP and LPS layer of a G- bacteria.
Term
P Ring
Definition
Anchor attached to the PG layer of a G- bacteria.
Term
S Ring
Definition
Anchor found in the space between the PG and the membrane of a G- bacteria.
Term
M Ring
Definition
Anchor attached to the membrane of a G- bacteria.
Term

No direct role.

Indirectly causes harm because it can move the bacteria to a different part of the body.

Definition
Role the flagellum plays in causing harm.
Term
NONE. No antiflagella medications.
Definition
Role the flagellum plays in treatment of infection.
Term
Pili
Definition
Fimbrae
Term
Pili
Definition
small hairlike structures around the cell
Term

Attachment to:

1. Food source

2. Prevent flushing out

3. Another bacteria

Definition
Function of the Pili
Term
Protein called pilin
Definition
Chemical composition of Pili
Term
No direct role. Indirectly, pili cause harm because they keep bacteria from being flushed out of the body.
Definition
Role pili play in causing harm.
Term
Mouth and intestine.
Definition
Places  a bacteria with pili will attach so as to obtain a food source.
Term

Mouth

Upper respiratory tract

 Lower digestive tract

 Lower urinary tract

 Lower genital tract

Definition
Places bacteria with pili will attach to prevent from being flushed out of the body.
Term
Sexual conjugation, for the purpose of exchanging genetic material via Fpilus.
Definition
Reason bacteria attach to one another.
Term
Neisseria gonorrhoeae
Definition
Example of a bacteria that uses pili to keep from being flushed out of the system by producing 4 different types of pili.
Term
Attach bacteria to mucous membrane in the genitourinary tract.
Definition

Purposes of the 1st kind of pili in

Neisseria gonorrhoeae.

Term
Attach bacteria to the white blood cells (neutrophils).
Definition

Purpose of the 2nd type of pili in

Neisseria gonorrhoeae

Term
Attach bacteria to sperm.
Definition

Purpose of the 3rd type of pili in

Neisseria gonorrhoeae.

Term
Attach bacteria to other bacteria via the Fpili.
Definition

Purpose of the 4th type of pili in

Neisseria gonorrhoeae.

Term

NONE

 

Ex: gonorrhea without pili spread from reproductive system into other parts of the body and may cause more harm.

Definition
Role pili play in treatment of infection.
Term
Regulate cell activity and determine all cell characteristics.
Definition
Purpose of the nuclear material in a bacteria.
Term
DNA
Definition
Chemical makeup of genetic material in bacteria.
Term
Double stranded, circular, not inside the nucleus.
Definition
Structure of DNA in bacteria.
Term
Enzymes
Definition
Control all cellular chemical reactions.
Term
Proteins
Definition
What DNA codes for....
Term
No direct role, but DNA is responsible for everything a cell does, so there is an indirect role.
Definition
Role nuclear material plays in causing harm.
Term

Some antibiotics can damage DNA/RNA.

 

nalidixic acid

antiviral meds target RNA/DNA

Definition
Role nuclear material plays in treatment of infection.
Term
plasmids
Definition
extrachromosomal pieces of DNA used to transfer specific information across the Fpilus.
Term
Site of protein synthesis.
Definition
Function of ribosomes.
Term
polyribosomes
Definition
Ribosomes found in groups of two or more.
Term

50s unit

30s unit

Definition
Names of the large and small portions of a bacterial ribosome.
Term
Protein and RNA
Definition
Chemical makeup of ribosomes.
Term
No direct role but indirectly, some bacteria produce protein poisons called exotoxins.
Definition
Role ribosomes play in causing harm.
Term
exotoxin
Definition
Poisonous proteins produced/released by living G+ and G- bacteria.
Term

tetanus toxin (tetanospasmin)

 

botulism toxin (botox)

 

anthrax toxin

 

Definition
Examples of exotoxins.
Term

Some antibiotics will damage ribosomes (broad spectrum).

 

Some antiseptics and disinfectants can denature proteins in ribosomes.

Definition
Role ribosomes play in treating infection.
Term

streptomycin

erythromycin

azithromycin

tetracycline

doxycycline

Definition
Broad spectrum antibiotics that target ribosomes in both G+ and G- bacteria.
Term

alcohols

heavy metals

halogens

aldehydes

ethylene oxide gas

Definition
Five types of antiseptics and disinfectants used to denature proteins and target ribosomes.
Term

alcohols

heavy metals

halogens

aldehydes

ethylene oxide gas

Definition
5 Types of antiseptics/disinfectants that denature proteins and target ribosomes in bacteria.
Term

methyl alcohol

ethyl alcohol

isopropyl alcohol

Definition
Three examples of alcohols
Term

silver nitrate - used to prevent opthalmia neonatorum, blindness due to gonnorhea in newborns.

 

mercury - topical antiseptic

mercurochrome and merthiolate

Definition
Two heavy metals used to treat bacterial infections that target the ribosome.
Term

fluorine - toothpaste, drinking water

chlorine - drinking water, pools

iodine - skin antiseptic

bromine - hot tubs and whirlpool baths

Definition
Four halogens used to denature proteins in bacteria and examples of their uses.
Term
bleach
Definition
sodium hypochlorite
Term
chlorinated lime
Definition
calcium hypochloride
Term

tincture of iodine

iodaphor

Definition
two examples of iodines used to denature protein in bacteria
Term
capsule
Definition
slime layer or mucocomplex
Term

Prevent phagocytosis by white blood cells.

Attachment of bacteria to body cells.

Site of stored sugars.

Prevent dehydration.

Prevent viruses from infecting bacteria.

Definition
Functions of the capsule.
Term
Complexes of sugar called mucocomplex or mucopolysaccharide.
Definition
Chemical composition of capsule.
Term

Direct role in causing harm...

When capsules dry up and become sticky, they can congest air spaces - especially in bronchi and lungs.

 

Indirectly, capsules prevent phagocytocis.

Definition
Role capsules play in causing harm.
Term
NONE
Definition
Role the capsule plays in treating infection.
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