Term
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Definition
| insert fiber connected to light source, directly into brain of animal. allows to illuminate target cells |
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Term
| benefits of zebrafish as model organism |
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Definition
-transparent embryos
-sequenced genome
-amenable to genetic manipulation
-complex behaviors (swimming, biting etc) |
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Term
| genetic targeting: mosaic expression of ChR2 |
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Definition
| express ChR2-EYFP in somatosensory neurons, Rohon-Beard (RB) and trigeminal neurons by means of an ehancer element |
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Term
| Activating ChR2 in a single sensory neuron induces behavior exp |
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Definition
| blue light induces an escape-like response in ChR2-expressing fish |
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Term
| Rebound swimming behavior exp |
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Definition
-express NpHR almost everywhere in CNS
-activate NpHR via global illumination prevents larvae from swimming
-however, the larvae starts swimming after the offsets of photostimulation |
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Term
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Definition
use optic fiber to isolate small regions in CNS.
-determine if NpHR mediated inhibition
of specific cells induces rebound swimming
=rebound swimming behavior was mapped to caudal hindbrain |
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Term
| potential optogenetic therapies |
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Definition
epilepsy-stop the spread of epilepsy activity via NpHR-mediated inhibition
Depression-block neuronal activity in cingulate area
excite neurons in NA via ChR2 activation |
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