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Bio 221 Exam 1
Purdue University Bio 221 Exam 1
110
Biology
Undergraduate 2
09/20/2012

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Cards

Term

Cyanobacteria

Purple Bacteria

Flavobacteria

Thormotago

Aquifex

Gram positive

Definition
Bacteria
Term

Theromproteus

pyrodictium

Methanacoccus

Thermococcua

Methanobacterium

Halobacterium

Thermolasma

Methanopyrus

Definition
Archea
Term

Entamoebae

Slime Molds

Animals

Fungi

Plants

Ciliates

Flagellates

Microsporidia

Diplomonads

Definition
Eukarya
Term
The size of prokaryotes is ...
Definition
Diffusion limited. They lack an endomembrane system.
Term

All about Bacteria

 

Bacteria size

Neuclear membrane

Cell Wall

cytoskeleton

Definition

0.3-2 micrometers

None

peptidoglycan present

yes- peptidoglycan synthases

 

Term

All about Archea

 

Size

Nuclear membrane

Cell Wall

Cytoskeleton

Definition

0.3-2 Microns

no

No peptidoglycan

Probably

Term

Eucarya

 

Size

Nuclear membrane

Cell wall

Cytoskeleton

Definition

5-50 microns

has a nuclear membrane

No peptidoglycan

Yes actin and tubulin

Term
Bacteria's cell wall contains...
Definition
Peptidoglycan
Term
Archea are...
Definition
Prokaryotes that live in extreme lifestyles
Term

Protazoa have what for feeding?

 

Definition
Endocytosis
Term
Viruses
Definition

Have no metabolism

Nucleic acid and a protein coat

May have an envelope

 

Term
Viroids
Definition

Only have RNA

and no Proteins

Term
Prions
Definition

Are an infectious protien

Have no nucleic acid

Term
Eukaryotes have a "true..."
Definition
Nucleus
Term
Protons
Definition
Have positive charge
Term
Neutrons
Definition
Have a neutral charge
Term
Electrons
Definition
Have a negative charge
Term
The electrons that orbit the nucleus...
Definition
Are arranged in orbitals of different energy levels
Term
What kind of electrons can participate in bond formation
Definition
Valence
Term
Strong bonds
Definition

Covalent

Polar covalent

Term
Covalent bonds
Definition
Have a difference in electronegativity of <0.5
Term
Polar covalent bonds
Definition
Have a difference in electronegativity in the ange of 0.5-1.6
Term
Weak bonds
Definition

Charge attractions

Ionic bonds

H bonds

Van der waals

Term
Ionic bonds
Definition
Have a difference in electronegativity that is > 1.6
Term
Hydrogen bonds
Definition

Only with N or O

Never with C

Term
Van der Waals interactions
Definition

Induced dipole

Hydrophobic interactions

Term
Molecules are held together with
Definition
Weak Bonds
Term
Cohesion
Definition
Molecules stick together via H bonds
Term
High heat capacity of water
Definition
Allows for evaporative cooling
Term
Solid water is less dense than liquid water
Definition
Ice floats because it expands
Term
Water dissolves ...
Definition
polar molecules
Term
Water surrounds
Definition
Non polar molecules
Term

Amphipathic molecules...

 

Definition

Have a hydrophobic end and a hydrophillic end

Have the ability to create micelles

Term
Water can...
Definition
Hydrate itself
Term
Macromolecules are polymers created by
Definition
Dehydration synthesis reactions
Term
Hydrolysis
Definition
Breaks down macromolecules
Term
Proteins are...
Definition
Polymers of amino acids
Term
non polar R groups
Definition

Glucine

Alanine

Valine

Leucine

Isoleucine

Methionine

 

Term
Aromatic R groups
Definition

Phenylalanine

Tyrosine

Tryptophan

Term
Polar uncharged R groups
Definition

Serine

Proline

Threonine

Cystine

Asparagine

Glutamine

Term
Negativly charged R groups
Definition

Aspartate

Glutamate

Term
Chirality
Definition

D- and L- steroeisomers

(mirror Images)

Term
Amino acids
Definition
Are joined by peptide bonds
Term
Hydrogen bonding is important in protein's______ structure
Definition
Secondary
Term
Tertiary structure is held together by hydrogen bonds and is responsible for the...
Definition
Folding of proteins
Term
Carbs are
Definition
polymers of monosaccharides
Term
Nucleic acids
Definition
are polymers of nucleotides
Term
5' end
Definition
Has a phosphate
Term
3' end
Definition
Has an OH
Term
H-bonds are needed to make
Definition
a double helix
Term
Lipids
Definition
are a condensation of fatty acids and glycerol
Term
Bent fatty acid
Definition
Cis
Term
Straight fatty acid
Definition
Saturated
Term
Slightly bent
Definition
Trans unsaturated
Term
Replacing one fatty acid in a lipid with a phospate gives you a
Definition
Phospolipid
Term
Resolution
Definition

How much detail can be seen

How close together can 2 objects be and still be separated in the image clearly

Term
Contrast
Definition

How distinct is the specimen from the background

 

Term
Enhances contrast
Definition

Phase contrast

DIC

Negative stain

Flouresent microscope

Term
Bright field microscope
Definition

Light passes evenly through a specimen

Most commonly used

Term
Dark field microscope
Definition

Special condenser is used

Light is cast upon the specimen from an angle

Used to illuminate very thin bacteria

No stain

Term
Phase contrast microscope
Definition

Phase plate retards some light and enhances other light

Enhances contrast of interior structures

Term
Differential interference Microscope
Definition

Usefule for veiwing for viewing protazoa in 3-d

Light is separated by a prism and polarized

Term
Fluorescent Microscope
Definition
Uv lights flouresently labeled specimen
Term
Pink, thin, and negative
Definition
All have n gram Negative
Term
Red acid fast
Definition
Like fast red race car
Term
Cell membranes are...
Definition
Semipermeable
Term
Aquaporins
Definition
Allow free passage of water and small non polar solutes
Term
Polar molecules and ions do
Definition
not pass freely
Term

Large molecules

 

Definition
Require a large complex of transporters
Term
Water flow
Definition
Results in osmosis across the membrane
Term
Ion separation results in
Definition

Motive gradients (PMF) proton motive force

Both charge and concentration differences

Term
PMF (proton Motive force) is created by
Definition
Proton pumps
Term
Bacteriorhodopsin
Definition
Uses light energy
Term
Respiratory chain
Definition
Uses energy via redox reactions
Term
H+ entering the cell is used for/to
Definition

Make ATP

Transport

Flagella movement

Term
Gram Positive
Definition

Thick Peptidoglycan

Purple when stained

Term
Gram Negative
Definition

Thin PG periplasm

Pink when stained

Term
Mycobacteria
Definition
Waxy Mycolic acid
Term
Mycoplasma and spiroplasma have no cell wall
Definition
Term
Archea have a cell wall of
Definition
Protein, Pseudo PG, and chondroitin
Term
Peptidoglycan is a highly
Definition
crosslinked Continuous polymer
Term

LPS

Lipopolysaccharide

Definition
Endotoxin
Term
Vessicles of LPS from the outer membrane can
Definition
Transfer cargo to other cells
Term
Secondary transporters use_____ for energy
Definition

PMF

examples: symports antiports, Uniports

Term
Primary Transporters use ____ for energy
Definition

ATP

Examples: APC, PTS

Term
Group translocation
Definition
Bring sugar into the cell
Term

General secretory pathway

(GSP)

Definition
Pump Protein out
Term
Passive transport
Definition

Uses energy gradient

Ex: aquaporin, Glyceroporin

Term

Major Facilitator superfamily

(MFS)

Definition
Proteins use energy from the gradient of one ion to move another against it's gradient
Term
ABC
Definition
ATP Binding cassette
Term

PTS

Primary active transporter

Definition

Phosphotransferase

Uses a phosphate relay to transport glucose (sugar)

Term
Type I and type III transporters cross
Definition
Both membranes at one time
Term
Type I transporter uses ATP or PMF?
Definition
PMF
Term
Type III secretion is like a...
Definition
syringe
Term
Capsules and slime layers function
Definition
Attached to surface prevent phagocytosis
Term
Most flagellar structural proteins are closely related to
Definition
T3SS proteins
Term
Chemotaxis is based on
Definition
the direction of flagellar rotation
Term
Flagella in bundle
Definition
Run CCW E. Coli
Term
Flagella Disarryed
Definition
Tumble CW E. Coli
Term
Chemotaxis is controlled by
Definition
Continued increase in frequency of contact with chemoattractant
Term
Switch between Y and Y-P phosphoralation causes
Definition
Reverse of moto spin
Term
Pili
Definition

Used for attachment to surfaces

Nanowires

Term
Volution stores
Definition
Phosphate granules
Term
Glycogen granules store
Definition
Carbon
Term
Phycobillisomes store
Definition
Pigments for photo synthesis
Term
Microcomparment MPCs store
Definition
Enzymes and their substrates
Term
Formation of endospores is a
Definition
7 stage process
Term
endo spore stages
Definition

0-Normal growth

1-Bacterial division

2-Assymetrical separation

3-Engulfment

4- Cortex

5-coat

6- lysis of mother cell

7- free spore

Term
Endospore cortex
Definition
Absorbs water prevents boiling
Term
endospore coat
Definition
protects against dehydration and UV radiation
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