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bio 20 a
chapter 14
26
Biology
Undergraduate 2
06/08/2010

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Term
Beadle and Tatums's Experiment
Definition
looked at the metabolic enzymes in hte bread mold Neurospora - led to the one gene, one enzyme hypothesis (a mutation causes an efect on only one ensyme). Now we say one-gene, one-polypeptide
Term
Uracil (U)
Definition
similar to tymine, but is in RNA. Lacks the Mythel (-CH3) group.
Term
Messanger RNA
Definition
carries a copy of a gene sequence in DNA to the site of protein synthesis at the ribosome
Term
Transfer RNA
Definition
carries amino acids to the ribosome for assembly into polypeptides. It associates with mRNA.
Term
Ribosomal RNA
Definition
catalyzes peptide bond formation and provides a structural framework for the ribosome
Term
Codons
Definition
a sequence of three consecutive nucleotides. Different codons encode for different amino acids.
Term
transcription
Definition
the information in a DNA sequence (a gene) is copied into a complementary RNA sequence
Term
Translation
Definition
an RNA sequence is used to create the amino acid sequence of a poly peptide
Term
anticodons
Definition
three nucleotides in transfer RNA that pair with a complementary triplet(a codon) in messenger RNA
Term
RNA polymerase
Definition
an enzyme that catalyzes RNA from DNA (Prokaryotes and Eukaryotes) Don't require a primer and do not have proofreading functions.
Term
promoter
Definition
a DNA sequence to which RNA polymerase bind to initiate transcription
Term
elongation
Definition
the addition of monomers to make a longer RNA or protein during transcription of translation
Term
initiation
Definition
the beginning of transcription and translation
Term
termination
Definition
the end of transcription and translation. Specified by particular base sequences.
Term
start codon
Definition
the initiation signal for translation (AUG)
Term
stop codons
Definition
termination signals for translation (UAA, UAG, UGA)
Term
intron
Definition
a portion of the gene within the coding region that is transcribed into pre-mRNA but is spliced out prior to translation. Only in Eukaryotes.
Term
Exons
Definition
a portion of DNA molecules that are in eukaryotes that codes for part of a poly peptide.
Term
pre-mRNA
Definition
(precoursor-mRNA) initial gene transcript before it is modified to produce functional mRNA. Where introns and exons appear.
Term
G cap
Definition
a chemically modified GTP added to the 5' end of the mRNA, facilitates binding of mRNA to ribosome and prevents breakdown of mRNA.
Term
poly A tail
Definition
a long sequence of adenine nucleotides added after transcription to the 3' end of most eukaryotic mRNA
Term
RNA splicing
Definition
the last stage of RNA processing in eukaryotes, in which the transcripts of introns are excised through the action of snRPS
Term
Tree things that happen to mRNA in Eukaryotic cells before transcription:
Definition
1) G Cap added
2) Poly A tail added
3) intons are spliced out (RNA splicing) and exons are put together
Term
spliceosome
Definition
a large RNA-protien complex that cuts the pre-mRNA, releases the introns and joins the ends of the exons together to produce a mature mRNA
Term
ribosome
Definition
where translation takes place. Holds mRNA and tRNA in the right positions so the poly peptide chain can be assembled. Three sites: A (amino acid) P (polypeptide) and E (exit)
Term
peptidyl transferase activities
Definition
when the large ribosome subunit 1) breaks the bonds of tRNA and its polypeptide 2) catalyzes the formation of a peptide bond between the two amino acids
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