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Bio 152
Brogna, Hotchin,
17
Biochemistry
Undergraduate 1
11/14/2015

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Cards

Term
Cell Fractionation
Definition
Common technique used to seperate cells int otheir compartments
Isotope labelling can be used to follow compound
Membranes are treated with detergent to solubilise membranes
Term
3 features of a cell
Definition
Plasma membrane
Cell consumes energy
Replicates
Term
Cell Theory
Definition
All organisms are composed of cells
The cell is the basic unit of life
All cells arise from pre-existing cells
Term
Matthias Scleiden
Definition
1838-Concluded plant tissues are made of cells and all plants developed from a single cell
Term
Theodor Schwaan
Definition
1839-Concluded animal tissues are made of cells
Term
Rudolph Virchow
Definition
1855-Stated that all living things come from other living things
Term
Electron Microscope
Definition
1931
Uses a beam of electrons that is deflected and focussed by an electromagnetic field
Much higher resolution and magnification
can form a 3D image
Term
Double Membrane Organelles
Definition
Nucleus
mitochondria
ER
Golgi Body
Chloroplasts
Term
Single Membrane Organelles
Definition
Lysosomes
Vacuoles
Term
Organelles without a membrane
Definition
Ribosome
nucleolus
Term
Optical Microscopy
Definition
Contrast enhancing techniques
cell staining
immunostaining
fluorescence microscopy and fluorescent protein tagging
Term
Ribosomes are made in the
Definition
nucleolus
Term
Lysosomes
Definition
Primary catabolic compartments of a cell, replete with enzymes that carry out intercellular digestion
Term
mitochondria
Definition
membrane bound organelles found in eukaryotic cells, produce most of a cells ATP by oxidative phosphorylation, have a DNA genome and a secondary inner membrane
Term
Actin
Definition
Globular protein that binds to ATP which regulates polymerisation
Polar, has a + end and a - end
Grows from + end 5x faster than -end, regulated by ATP
Term
Microtubles
Definition
long tubes made of tubulin proteins
globular
made of alpha and beta tubulin dimers
continuously polymerised at one end and depolymerised at the other, forming an equilibrium
Term
Intermediate Filament
Definition
Thick, rope-like fibers made of heterogenous fibrous proteins
eg, keratin
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