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BIO 111 Lab Practical 2
N/A
45
Biology
Undergraduate 1
03/24/2010

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Term
How did 'fast plants' get their name?
Definition
they have a short life cycle
Term
Diploid number of chromosomes in fast plants (Brassica rapa)
Definition
20
Term
the gene that controls the synthesis of purple pigment in fast plants
Definition
anthocyanin
Term
in brassica rapa (fast plants) the genetic locus is named after the wild type/mutant form of the trait?
Definition
mutant
Term
in the fast plants lab, we analyzed the results of a ____hybrid cross
Definition
mono
Term
when doing a chi-squared test, when do you reject a hypothesis?
Definition
when the probability is < 0.05
Term
the fast plants (zea mays) cross is a ___hybrid cross
Definition
di
Term
___ produces gene combinations in the gametes of an organism that are different from the gene combinations that were in the gametes that fused to make that organism
Definition
recombination
Term
relationship between % recombination and genetic distance
Definition
1% recombo = 1cM
Term
2 phenotypes invovled in a test cross
Definition
heterozygous x homo recessive
Term
For a reaction to proceed spontaneously, the free energy of the products/reactants must be less than the free energy of the products/reactants; the reaction must be ____.
Definition
products
Term
initial input of energy that must be supplied before a reaction that involves covalent bonds will proceed
Definition
activation energy
Term
____ is required to brin ght reacting molecules close enough and in the proper orientation for the reaction between them to proceed.
Definition
activation energy
Term
___ can greatly reduce the activation energy needed for reactions.
Definition
catalysts
Term
___ are biological catalysts. They are usually what kind of molecule?
Definition
enzymes ; proteins
Term
____ is the enzyme that normally catalyzes the removal of phosphate (PO4) from molecules within a cell
Definition
alkaline phosphatase
Term
the greater the concentration of pNP in a solution, the more blue light it absorbs, that is, the higher its ______.
Definition
absorbance
Term
Spec 20 operates in the range of ___-___ nm. It has six parts: ___________-
Definition
400-700nm ;; light source, monochromator, test tube, light detector, amplifier, readout
Term
three independent variables and one dependent variable in the enzyme lab. what's the relationship between them?
Definition
enzyme concentration, initial substrate concentration, pH :: initial rate of reaction :: we expect changes in each ind variable to cause the initial rate of the reaction to change
Term
blue light is at what wavelength
Definition
405 nm
Term
What is the relationship between enzyme concentration and initial reaction rate?
Definition
linear
Term
what is the relationship between initial substrate concentration and initial reaction rate?
Definition
rxn rate increases quickly initially until [S] reaches about 0.8mM, where it reaches saturation and the rxn rate levels off
Term
What is the relationship between pH and initial reaction rate?
Definition
rxn rate increases until about pH 10 and then it decreases... soln becomes too basic after that
Term
___ is typically thought to be the start of development
Definition
fertilization
Term
fertilization accomplishes what 2 things?
Definition
activates the oocyte (or egg) and provides the means by which the haploid set of chromosomes of the sperm joins the haploid set of chromosomes of the egg
Term
when the haploid chromosomes of the mom join with the haploid chromosomes of the dad, a ___ is created.
Definition
zygote
Term
during fertilization, a ___ lifts off the surface oft he egg soon after the sperm makes contact
Definition
fertilization membrane
Term
2 mechanisms to protect against polyspermy ... plus one more found specifically in sea urchins
Definition
electrical block followed by fertilization membrane ... in sea urchins, the eggs and sperm are released into the water so that they dilute and there's less chance of polyspermy
Term
progression of events during and after fertilization of sea urchin eggs
Definition
sperm enters egg, blister forms as beginning of fertilization membrane, head of sperm swells and becomes male pronucleus (female pronucleus is the nucleus of the egg), DNA sythesis, RNA synthesis, protein synthesis, cleavage of zygote
Term
cleavage in sea urchins is ____, which means?
Definition
holoblastic ;; the cleavage furrows cut completely and quickly through the embryo
Term
order in terms of least to most yolk: chicken, sea urchin, frog
Definition
sea urchin < frog < chicken
Term
sea urchin: 16-cell stage
Definition
morula
Term
after the morula stage, the ball of cells forms a cavity called the ____, thereby becoming a ____.
Definition
blastocoel :: blastula
Term
gastrulation in echinoderms begins with a few cells of the vegetal plate (the ____ cells) detaching and migrating into the interior of the embryo. These are ___ cells that will form the skeleton of the larva.
Definition
primary mesenchyme cells :: mesoderm cells
Term
mesoderm cells eventually become the ____ of the sea urchin
Definition
skeleton
Term
the endoderm in the sea urchin becomes the ____
Definition
primitive gut (archenteron)
Term
the ectoderm in the sea urchin embryo becomes the ____.
Definition
outer regions of the larva
Term
whats the difference between an egg and a zygote?
Definition
a zygote is a fertilized, diploid egg
Term
in echinoderms and amphibians, cleavage begins at the ___ pole and proceeds toward the ___ pole
Definition
animal --> vegetal
Term
in amphibians, the blastocoel occupies only the ___ hemisphere. this is different from echinoderms in which it occupies both hemispheres.
Definition
animal
Term
in echinoderms, gastrulation begins at the ___ pole. where does it begin in amphibians?
Definition
vegetal :: gray crescent
Term
as gastrulation proceeds in amphibians, the blastocoel is gradually reduced in volume by the ____
Definition
invaginating mesoderm and endoderm cells
Term
the small amount of non-yolk cell at the animal pole of a bird egg is called the ____
Definition
blastodisc
Term
Bird development characteristics of stages
Definition
16-18 hr stage = primitive streak :: 48 hr embryo = sea horse :: 72 hour embryo = bigger, curled up sea horse
Term
what is gastrulation? what does it accomplish immediately? how is it accomplished?
Definition
it is the method by which the organ systems are formed but immediately it creates the 3 germ layers. it is accomplished by successive cell divisions and is initiated by seaparation of cells from the lower surface of the blastoderm
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