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Definition
a sequence of nucleotides that code for an RNA molecule |
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Definition
making an RNA molecules complimentary to a sequence of DNA |
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For transcription what must happen to the DNA |
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Definition
the DNA must separate and one strand, the template stand, is used to make the RNA molecule |
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Definition
- it is a single stranded molecule
- has the sugar ribose in the nucleotides ( not deoxyribose)
- it contains the base uracil (U0 in place of thymine
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Why is RNA more fascinating than DNA |
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Definition
it has more functions
ex: making proteins |
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What is messenger RNA (mRNA) |
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Definition
- carries the code for making a protein
- the template that will be further read in translation
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Definition
the code on the mRNA that is expressed in words three nucleotides long |
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What is ribosomal RNA (rRNA) |
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Definition
- complex with proteins to form ribosomes
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Definition
-responsible for synthesizing all of the proteins in the cell by reading mRNAs
- composed of large and small subunits that sit on the mRNA like a sandwich |
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What is transfer RNA (tRNA) |
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Definition
carry amino acids to the ribosome
- one end carries the anticodon
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Term
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Definition
its complimentary to the codon on the mRNA, it determines which amino acid goes in which spot in the "sentence" |
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where does transcription and translation happen for Eukaryotes |
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Definition
- transcription- in the nucleus
- translation- cytoplasm
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Term
After the gene is transcriber, the protein-coding RNA goes... |
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Definition
to be translated into a protein |
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Term
What enzyme is needed for transcription |
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Definition
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What are all the things the RNA polymerase can do |
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Definition
- unzip DNA
- utilizes a single stranded template
- polymerizes to make new RNA
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Term
What are the regions of a gene |
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Definition
- the promoter- where RNA polymerase binds
- start- codon
- actual coding sequence
- stop codon- termination signal that stops transcription
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Term
What are the 3 phases of transcription |
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Definition
- Initiation
- Elongation
- Termination
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What are the 3 phases of initiation |
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Definition
- attachment of the RNA polymerase to the promoter
- Opening of the DNA strands
- Start of RNA synthesis
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What are transcription factors |
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Definition
how the promote is recognized in Eukaryotes |
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Term
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Definition
a subunit in prokaryotes that is responsible for locating promoters |
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Term
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Definition
- the RNA polymerase base pairs RNA nucleotides to complimentary template DNA and polymerizes forming phosphodiester bonds between nucleotides
- moves in the downstream direction
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Term
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Definition
- Once the RNA polymerase reaches the terminator sequence, transcription halts, the RNA polymerase disassociates, and the DNA strands re-anneal
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Term
After termination is eukaryotes, the RNA must |
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Definition
go through post- transcriptional processing before it can be considered true mRNA capable of being translated |
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What are the two pieces that must be added to eukaryote transcripts |
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Definition
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Term
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Definition
its 50-250 nucleotides ling that serve as a protective bumper against the RNA degradation before mRNA can be translated |
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Term
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Definition
the 5' carbon of 7-methylgaunine is bonded to the 5' carbon of the mRNA and it is crucial for the start of translation to occur in eukaryotes |
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Definition
the "coding" regions within a gene |
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Term
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Definition
extraneous parts of the message and before a transcript can be translated, the introns must be removed |
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Term
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Definition
the process where introns are removed |
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Term
Why are there introns if they are just spliced out |
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Definition
- provide a mechanism for gene evolution
- Alternative splicing
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Term
What is Alternative splicing |
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Definition
- just because an intron is present does not mean it gets spliced out
not all get spliced out in the same order
- So, the cell has roughly 3x the number of proteins as it has genes to code for them thanks to alternative splicing |
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