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BIO 1107 Chap 15
Gene Expression and Transcription
31
Biology
Undergraduate 1
12/02/2024

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Term
What is gene defined as
Definition
a sequence of nucleotides that code for an RNA molecule
Term
What is transcription
Definition
making an RNA molecules complimentary to a sequence of DNA
Term
For transcription what must happen to the DNA
Definition
the DNA must separate and one strand, the template stand, is used to make the RNA molecule
Term
Characteristics of RNA
Definition
  1. it is a single stranded molecule
  2. has the sugar ribose in the nucleotides ( not deoxyribose)
  3. it contains the base uracil (U0 in place of thymine
Term
Why is RNA more fascinating than DNA
Definition

it has more functions

ex: making proteins

Term
What is messenger RNA (mRNA)
Definition
  • carries the code for making a protein
  • the template that will be further read in translation
Term
what are codons
Definition
the code on the mRNA that is expressed in words three nucleotides long
Term
What is ribosomal RNA (rRNA)
Definition
  • complex with proteins to form ribosomes
Term
what are ribosomes
Definition

-responsible for synthesizing all of the proteins in the cell by reading mRNAs

- composed of large and small subunits that sit on the mRNA like a sandwich

Term
What is transfer RNA (tRNA)
Definition

carry amino acids to the ribosome

  • one end carries the anticodon
Term
what is an anticodon
Definition
its complimentary to the codon on the mRNA, it determines which amino acid goes in which spot in the "sentence"
Term
where does transcription and translation happen for Eukaryotes
Definition
  • transcription- in the nucleus
  • translation- cytoplasm
Term
After the gene is transcriber, the protein-coding RNA goes...
Definition
to be translated into a protein
Term
What enzyme is needed for transcription
Definition
RNA polymerase
Term
What are all the things the RNA polymerase can do
Definition
  1. unzip DNA
  2. utilizes a single stranded template
  3. polymerizes to make new RNA
Term
What are the regions of a gene
Definition
  1. the promoter- where RNA polymerase binds
  2. start- codon
  3. actual coding sequence
  4. stop codon- termination signal that stops transcription
Term
What are the 3 phases of transcription
Definition
  1. Initiation
  2. Elongation
  3. Termination
Term
What are the 3 phases of initiation
Definition
  1. attachment of the RNA polymerase to the promoter
  2. Opening of the DNA strands
  3. Start of RNA synthesis
Term
What are transcription factors
Definition
how the promote is recognized in Eukaryotes
Term
What is a sigma factor
Definition
a subunit in prokaryotes that is responsible for locating promoters
Term
What is Elongation
Definition
  1. the RNA polymerase base pairs RNA nucleotides to complimentary template DNA and polymerizes forming phosphodiester bonds between nucleotides
  2. moves in the downstream direction
Term
What is termination
Definition
  • Once the RNA polymerase reaches the terminator sequence, transcription halts, the RNA polymerase disassociates, and the DNA strands re-anneal
Term
After termination is eukaryotes, the RNA must
Definition
go through post- transcriptional processing before it can be considered true mRNA capable of being translated
Term
What are the two pieces that must be added to eukaryote transcripts
Definition
  1. poly a tail
  2. the 5' cap
Term
What is the poly a tail
Definition
its 50-250 nucleotides ling that serve as a protective bumper against the RNA degradation before mRNA can be translated
Term
What is the 5' cap
Definition
the 5' carbon of 7-methylgaunine is bonded to the 5' carbon of the mRNA and it is crucial for the start of translation to occur in eukaryotes
Term
What are exons
Definition
the "coding" regions within a gene
Term
what are introns
Definition
extraneous parts of the message and before a transcript can be translated, the introns must be removed
Term
what is splicing
Definition
the process where introns are removed
Term
Why are there introns if they are just spliced out
Definition
  1. provide a mechanism for gene evolution
  2. Alternative splicing
Term
What is Alternative splicing
Definition

- just because an intron is present does not mean it gets spliced out

not all get spliced out in the same order

- So, the cell has roughly 3x the number of proteins as it has genes to code for them thanks to alternative splicing

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