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Bio 107 Exam 4
Prokaryotes, Viruses, and Protists
92
Biology
Undergraduate 2
05/03/2010

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Term
T or F: Prokaryotes have a plasma membrane.
Definition
True. It enclosed the cytoplasm.
Term
Fimbriae
Definition
Protein attachment structures on the surface of some prokaryotes. shorter and more numerous than sex pili. also called attachment pili
Term
Nucleoidd
Definition
region where prokaryote DNA is located. Not enclosed by a membrane.
Term
Ribosomes
Definition
synthesize proteins in all cells
Term
Plasma Membrane
Definition
membrane enclosing the cytoplasm that regulates passage of materials into and out of the cell.
Term
Cell Wall
Definition
regid structure outside of plasma membrane. maintains cell shape, provides physical protection and prevents the cell from bursting in a hypotonic environment.
Term
Capsule
Definition
A sticky, jellylike outer coating of many prokaryotes. made of polysaccharide or protein and enables prokaryotes to adhere to their substrate or to other individuals in a colony. Also protests against dehydration and shields from attacks.
Term
Sex pili
Definition
appendages that pull two cells together prior to DNA transfer from one cell to another
Term
Flagella
Definition
Locomotion organelles of some bacteria. can be focused on either end or around the cell
Term
3 common shapes of bacteria
Definition
spheres (coci), rods (bacilli), and spirals
Term
Peptidoglycan
Definition
network of modified-sugar polymeres cross-linked by short polypeptides. Makes up bacteria cell walls and is not present in archaeal cells
Term
Gram-positive bacteria
Definition
bacteria with simple walls made of a large amount of poptidoglycan
Term
Gram-negative bacteria
Definition
have less peptidoglycan and are more structurally comples. contain lipoplysaccharides in outer membrane. more resistant to defenses because of outer membrane and toxicty of lipopolysaccharides
Term
taxis
Definition
movement towards or away from a stimulus.
Ex: towards other organisms in same family or towards nutrients.
Ex: away from harmful chemicals
Term
Prokaryotic genome vs. Eukaryotic
Definition
consists of a circular chromosome with fewer proteins than eukaroytic DNA
Term
Plasmids
Definition
small circular chromosomes of separately replicating DNA in prokaryotes
Term
Three key Prokaryotic Features and benefits
Definition
1) they're small
2) they reproduce by binary fission. allows for quick reproduction and increases chances of mutations
3) they have short generation times. allows for faster reproduction rates causing more genetic variation.
Term
Endospores
Definition
resistant cells developed when an essential nutrient is lacking. Chromosome is copied and surounded by a tough wall, water is removed, and metabolism halts. Rest of the cell disintegrates and then the endospore can be stored for long periods of time
Term
Bacteriophages
Definition
viruses that infect bacteria
Ex: T4
Term
e ways of prokaryote DNA exchange
Definition
Transformation, Conjuction, and Transduction
Term
3 ways of prokaryote DNA exchange
Definition
Transformation, Conjuction, and Transduction
Term
F Factor
Definition
piece of DNA that allows for conjugation
Term
F+ cells ______ and F- cells _______
Definition
act as donor, act as recipient, becomes converted into F+
Term
Hfr Cell
Definition
has F factor built into chromosomes
Term
Resistance genes
Definition
code for enzymens tha specifically destroy/hinder effectiveness of cetain antibiotics. carried on R plasmids
Term
R plasmids
Definition
carry resistance genes
Term
Viruses _______ reproduce/carry out metabolic activities outside of host cell
Definition
cannot
Term
Dimitri Ivanswsky
Definition
passed tobacco sap through a bacteria filter. discovered that it was either very small bacteria or something that bacteria produced that was infecting tobacco plants
Term
Martinus beijerinck
Definition
showed viruses can reproduce and is credited with being the first to voice the concept of a virus
Term
Virus genomes may consist of
Definition
singe or double stranded DNA or RNA
Term
Virus genome is usually orgainzed as a:
Definition
single linear or circular molecule of nucleic acid
Term
Casid
Definition
a protein shell enclosing a viral genome. is either rod shaped, polhedral, or more complex based on the virus. Made of protein subunits called capsomers
Term
Capsomers
Definition
protein subunits that make up a virus capsid
Term
Viral envelopes
Definition
Envelop virus and are derived from the membrane of host cell and contain host phospholipids and membrane proteins.
Term
Why can viruses only reproduce in a host cell?
Definition
They lasck metabolic enzymens and equipment to make proteins
Term
Host range
Definition
The variety of hosts that a virus can infect. Have a lock and key fit between a specific receptor site on the host and the virus that was evolved by the virus.
Term
Lytic cycle
Definition
phage reproductive cycle that ends with the death of the host cell by lysis
Term
Virulent phage
Definition
phage that reproduced with only the lytic cycle
Term
Stages of the lytic cycle
Definition
1) T4 used tail fibers to attach to speccific receptor site
2) sheath of tail contacts, ejecting phage DNA into cell. DNA is hydrolyzed
3) page DNA directs duplication of phage proteins and genome
4) phage is assembled from 3 different proteins, 1 each for the head, tail, and tail fibers.
5) enqyme is produced that damages cell walls of host, allowing liquid ot enter until the cell bursts.
Term
Bacteria defense against phages
Definition
Bacteria restriction enzymes cut up phage DNA before it reproduces
Term
Lysogenic cycle
Definition
allows replication of phage without destrying the host
Term
Temperate phages
Definition
undergo lysogenic and lytic cycles
Term
Lysogenic cycle process
Definition
1) phage attaches and injests DNA
2) DNA incorporates into bacteria chromosome. Viral DNA becomes a prophage and most of it goes inactive
3) BActeria reproduces normally, copying and transmitting the phage
4) environmental signal triggers a switch to the lytic cycle
Term
Prophage
Definition
viral DNA incorporated into a bacteria chromosome during lysogenic cycle
Term
Glycoproteins
Definition
spikes on outside of a virus that allow for attachment to a host. made from ribosomes bound to the ER of host and sugars added by enzymens in the ER and Golgi A.
Term
Reproductive Cycle of RNA Virus
Definition
1) Glycop on viral env. bind to specific recpt. molec. on host
2) capis and viral genome enter cell. capsid is dgested
3) viral genome is template for complementary RNA synthesis and mRNA
4)New RNA copies made using comp. RNA strand as template
5) Comp RNA strand functions as mRNA and is translated into capsid and glycop for viral env.
6) vesicles transpot glycop to plasma membrane
7) Capsid assembles around each viral genome
8) new birus buds from cell through exocytosis
Term
Retrovirus and example
Definition
Class VI, have most complicated reproductive cycle of RNA animal viruses. Ex: HIV
Term
Reverse transcriptase
Definition
enzyme found in retroviruses that transcribes RNA template into DNA (backwards of normal)
Term
Retroviruses contain
Definition
2 indentical single-stranded RNa and 2 molecules of reverse transcriptase
Term
Reproductive cycel of Retrovirus (HIV)
Definition
1) Glycop allows bindage to white blood cells
2)Virus enters by endocytosis, capsid proteins removed releasing viral proteins and RNA
3)Reverse Transc. catalyzes synthesis of DNA strand complementary to RNA
4)Rev. Transc. catalyzes synthesis of DNA strand complementary to DNA 1
5)Double stranded DNA incorporated as provirus into cells DNA
6) Proviral genes transcribed into RNA for next viral generation and mRNA for translation into viral protein (capsid protein, rev. transc., envelope glycop)
7) vesicles transport glycop to cells plasma memb.
8) capsides assemble around viral genomes adn rever. transc molec.
9) exocytosis creases new virus
Term
When is it believed that viruses evolved and why?
Definition
evolved after other life forms because they're dependent on other life forms
Term
Provirus
Definition
Retrovirus RNA that is converted into double stranded DNA and incorporated into hosts DNA. mainly inactive but can have some active genes
Term
Transpoons
Definition
DNA segments that can move from 1 location to another within a cells genome
Term
mimivirus
Definition
double stranded DNA virus that is very large. has traits that were previously thought of as trains of only gellular genome
Term
What does the damage caused by a virs depend on?
Definition
The ability of the infected tissue to regenerate by cell division. Intestinal tissue is more resistant because it regenerates quickly. Nervous tissue is vulenerable because it doesnt regenerate
Term
Vaccine and how it works
Definition
harmless variant of a pathogen that stimulates the immunse systems defenses against the harmful version of the pathogen
Term
What is a target for the development of viral vaccines?
Definition
the enzymes that are encoded in some viruses
Term
Emerging Viruses and examples
Definition
new viruses or viruses that appear suddenly
Ex: HIV, Ebola (entral Africa, causes hemorrhagic fevor), West Nile Virus, SARS (China, 2002)
Term
3 Processes that cause emergence of viral diseases
Definition
1) Mutation of existing viruses. RNa viruses have high mutation rated due to the lack of proofreading in RNA replication. Causes the flue outbreak every year.
2) Dissemination of viral disease in small isolated human populations. Ex: AIDS
3)Spread of viruses from animals to humans. 3/4 of new diseases emerge this way. Ex: swine flu, bird influenza
Term
Influenza A
Definition
infects range of animals and caused 3 epidemics in humans including the "Spanish flu" that killed 40 million ppl
Term
2 ways of spreading plant viral infections
Definition
1) horizontal transmission: plant infected from external source of virus
2) Vertical transmission: inherited from parent. Asexual and sexual
Term
T or F. There are cures for most viral plant diseases.
Definition
False
Term
Viroids
Definition
circular RNA molecules that infect plants. REplicate in host plant cells using host cell enzymes and cause errors in the growth system causing stunted growth and abnormal development
Term
Prions, transmission, theory of how they work, and strengths of prions
Definition
infectious proteins that cause degenerative brain diseases including Mad Cow disease. Mostly transmitted through food (meat). Believed to be a misfolded protein that then converts normal proteins into misfolded proteins, creating chains of prions. Have an incubation period of at least 10 years, are virtually indestructible.
Term
T or F. Protists are eukaryotes
Definition
True
Term
Contractile Vacuole
Definition
Vacuole found in protists that pumps excess water from the cell
Term
How do protists get nutrients?
Definition
Heterotrophically, autotrophically, and mixotrophically
Term
5 supergroups of Protists
Definition
1) Excavata
2) Chromalveolata
3) Rhizaria
4) Archaeplastida
5) Unikonta
Term
Excavata
Definition
Protist supergroup composed of protists with modified mitochondria and unique flagella. Clades: Diplomonads, Parabasalids, and Euglenozoans
Term
Chromalveolates
Definition
Protist supergroup that may have originated by secondary endosymbiosis. Clades: Alveolates (Dinoflagellates, Apicomplexans, and Ciliates) and Stramenophiles (Oomycota, Diatoms, Brown Algae, and Golden Algae)
Term
Rhizarians
Definition
Protist supergroup defined by DNA similarities. Clades: Forams (Cercozoans) and Radiolarians. AMOEBAS
Term
Archaeplastida
Definition
Protist supergroup. consists of Red algae and green algae. closest relatives of land plants
Term
Unikonts
Definition
Protist supergroup that includes prtists that are related to fungi and animals. Clades: Ameobozoans (Entamoebas, Slime Molds, and Gymnamoebas) and Opisthokonts
Term
Dioplomonads
Definition
- Excavata
- Has modified mitochondria called mitosomes that lack ETC and used anaerobic processes.
- Parasite
- Example: Giardia lamblia, inhabits intestines of animals
Term
Parabasalids
Definition
- Excavata
- Reduced mitochondria called hydrogenosomes that generate some energy anaerobically releasing hydrogen gas
- Trichomonas Vaginalis: STD
Term
Euglenozoans
Definition
- Excavata
- has spiral/crystalline rod of unknown fuction inside of flagella
- Consists of Kinetoplastids and Euglena
Term
Kinetoplastids
Definition
- Euglenozoan, Excavata
- spiral/crystalline rod inside of flagella
- 1 mitochondria and a Kinetoplast ( organized mass of DNA)
- has "bait and switch" defense where the surface prteins change with each generation
- Typanosoma: causes sleeping sickness
Term
Euglena
Definition
- Euglenozoan, Excavata
- spiral/crystalline rod inside of flagella
- has a pocket at the end with 2 flagella
- mixotrophs
- Euglena (example)
Term
Alveolates
Definition
- chromalveolata
- has a membrane bound sac (alveoli) under plasma membrane
- consists of dinoflagellates, amicomplexans, and ciliates
Term
Dinoflagellates
Definition
- Alveolates, Chromalveolata
- alveoli
- reinforced by cellulose plates
- causes red tide
Term
Apicomplexans
Definition
- Alveolates, Chromalveolata
- alveoli
- parasites of animals. has a complex of organelles designed to penetrate the host
- Example: Plasmodium, causes malaria, changes its surface proteins to avoid detection, requires 2 hosts b/c it has sexual and asexual stages
Term
Ciliates
Definition
- Alveolates, Chromalveolata
- alveoli
- has Cilia and 2 nuclei (micronuclei and macronuclei)
- reproduces thorugh conjugation and binary fission
Term
Stramenopiles
Definition
- part of Chromalveolata
- marine algae with flagellum (1 hairy and longer, 1 smooth and shorter)
- consists of Oomycota, Diatoms, Brown Algae, and Golden Algae
Term
Diatoms
Definition
- Stramenopiles, Chromalveolata
- marine algae with hairy and smooth flagellum
- has glass-like wall of silica for protection
- VERY pressure resistant
- causes biological carbon pump
- mostly asexual
Term
Brown Algae
Definition
- Stramenopiles, Chromalveolata
- marine algae with hairy and smooth flagellum
- largest and most complex algae (seaweeds)
- are thalluses (algae that is plant like)
- consist of a holdfast, stipe, and blade
- Example: Laminaria, eaten in soups and undergoes Alternation of Generations
Term
Oomycota
Definition
- Stramenopiles, Chromalveolata
- marine algae with hairy and smooth flagellum
- used to be classified as fungi
- cell walls of cellulose
- Example: Phytophthora infestans, causes potato late blight which turns the stalk and stem of potato plants to black slime
Term
Amoebas
Definition
- Cercozoans and Radiolarians, Rhizaria
- have threadlike pseudopodia
Term
Ameobozoans
Definition
- Unikonta
- have either lobe or tube shaped pseudopodia
- Consists of Entamoebas, slime molds, and gymnamoebas
Term
Entamoebas
Definition
- Amoebozoans, Unikonta
- has lobe/tube shaped pseudopodia
- Parasites that infect all vertibrates and some invertebrates
- Example: E. histolytica, caues amebic dysentery
Term
Slime Molds
Definition
- Amoebozoans, Unikonta
- has lobe/tube shaped pseudopodia
- consist of plasmodial slime molds and cellular slime molds.
Term
Red Algae
Definition
- Archaeplastida
- has phycoerythin, a red pigment that absorbs blue and green light, allowing them to grow at deep depths
Term
Green Algae
Definition
- Archaeplastida
- closely related to land plants.
- consists of chlorophyta and charophyceans
- has plant like chloroplasts
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